Human tfpi quantikine elisa kit

The plasma concentrations of MPO, NETs (MPO/DNA complexes), tissue factor pathway inhibitor (TFPI), thrombin–antithrombin (TAT) complexes, tissue plasminogen activator (tPA), tissue factor (TF), vWF, beta-defensin 1, and complement components C3, C5, C5a, and sC5b-9 were measured via enzyme-linked immunosorbent assay (ELISA). Assays used were the High-Sensitivity Myeloperoxidase Human Assay Kit (Aviscera Bioscience, Santa Clara, CA, USA), Human Neutrophil extracellular traps ELISA Kit (Bioassay Technology Laboratory, Zheijang, China), Human TFPI Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), Human tPA ELISA Kit, Human TAT complex ELISA Kit, Tissue Factor ELISA Kit and Human VWF ELISA Kit (AssayMax, St. Charles, MO, USA), ELISA Kit for Defensin Beta 1, ELISA Kit for Complement Component 3, ELISA Kit for Complement Component 5, ELISA Kit for Complement Component 5a, and ELISA Kit for Terminal Complement Complex C5b-9 (Cloud-Clone Corp., Katy, TX, USA). ELISA was performed according to the manufacturer’s instructions and measured using the Tecan Infinite 200Pro (Tecan Group, Mannedorf, Switzerland). TF activity levels were determined using the Human Tissue Factor Chromogenic AssaySense Activity Assay Kit (AssayMax, St. Charles, MO, USA) and measured using the Tecan Infinite 200Pro (Tecan Group, Mannedorf, Switzerland).