Insulin mouse elisa kit

Manufactured by Abnova

The Insulin (mouse) ELISA Kit is a laboratory equipment product designed to quantitatively measure insulin levels in mouse samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify insulin concentrations. The kit provides the necessary reagents and materials to perform the assay.

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Lab products found in correlation

Glycerol standard, by Merck Group (1 mentions) Triacylglycerol reagent, by Roche (1 mentions) Nefa kit, by Fujifilm (1 mentions) Ab195013, by Abcam (1 mentions) 6 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxy d glucose 2 nbdg, by Thermo Fisher Scientific (1 mentions) Px 478 s7612, by Selleck Chemicals (1 mentions) Anti c myc antibody 9e10, by Santa Cruz Biotechnology (1 mentions) Alpha ketoglutarate assay kit, by Abcam (1 mentions) Ae012, by ABclonal (1 mentions) Anti ha antibody, by Fortrea (1 mentions)

2 protocols using insulin mouse elisa kit

Quantifying Metabolic Markers in Mice

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Plasma insulin concentration was measured using an Insulin (mouse) ELISA Kit (Abnova, Taiwan) according to the manufacturer's instructions. Samples were analysed in duplicate, and the intra-assay coefficient of variance was below 10%.
Liver lipids were extracted using the Folch method 26 , as previously described 24 (link) . Plasma, liver extracts and glycerol standards (Sigma-Aldrich, MO, USA) were incubated with triacylglycerol reagent (Roche Diagnostics, Basel, Switzerland) using an in-house assay 24 (link) .
Plasma nonesterified free fatty acid (NEFA) concentrations were measured using a NEFA kit (WAKO, Osaka, Japan).

Li G., Chan Y.L., Wang B., Saad S., Oliver B.G, & Chen H. (2020). Replacing smoking with vaping during pregnancy: Impacts on metabolic health in mice. Reproductive toxicology (Elmsford, N.Y.), 96.

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Investigating HIF-1α Regulation and Metabolic Adaptations

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The antibodies used were as follows: anti-c-Myc antibody (9E10, 1:1000 for IB analysis, Santa Cruz), anti-HA antibody (1:5000 for IB analysis, Covance), anti-β-actin antibody (AC004, 1:20,000 for IB analysis, ABclonal), anti-HIF-1α antibody (A6265, 1:1000 for IB analysis, ABclonal), anti-GFP antibody (AE012, 1:1000 for IB analysis, ABclonal), anti-HIF-OH antibody (3434S, 1:1000 for IB analysis, Cell Signaling), anti-Arginine (glcnac) (ab195013, 1:1000 for IB analysis, Abcam), anti-His (H15, 1:500 for IB analysis, Santa Cruz), anti-Ldha antibody (A1146, 1:1000 for IB analysis, ABclonal), anti-Pkm2 antibody (GB11392, 1:1000 for IB analysis, Servicebio), anti-Vegf (GB11034, 1:1000 for IB analysis, Servicebio), anti-Slc2a1 (Glut1) antibody (GB11215, 1:100 for IF analysis, Servicebio), anti-Ki67 antibody (GB113030-2, 1:100 for IF analysis, Servicebio), anti-Insulin antibody (GB13121, 1:100 for IF analysis, Servicebio).
PX-478 (S7612) was purchased from Selleck. 6-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG), a glucose analog, was purchased from Invitrogen. Insulin (Mouse) ELISA Kit (KA3812) was purchased from Abnova. Alpha-Ketoglutarate Assay Kit (K677-100) was purchased from Biovision.

Xu C., Liu X., Zha H., Fan S., Zhang D., Li S, & Xiao W. (2018). A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein. PLoS Pathogens, 14(8), e1007259.

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