Fluoro care anti fade mounting medium

Manufactured by Biocare Medical

Sourced in United States

Fluoro Care Anti-Fade mounting medium is a laboratory product designed to preserve fluorescent signals in microscopy samples. It helps maintain the brightness and stability of fluorescent labels, preventing fading or quenching over time.

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Lab products found in correlation

Laser confocal microscope, by Nikon (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Anti p53, by Cell Signaling Technology (1 mentions) Anti her2, by Agilent Technologies (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (1 mentions) Fluoview fv1000 confocal microscope, by Olympus (1 mentions)

2 protocols using fluoro care anti fade mounting medium

Immunofluorescence Analysis of MUC13 and Oncoproteins

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Immunofluorescence staining was performed to determine the effect of miR-145 transfection on the protein level of MUC13 and other related key oncogenic proteins. Cells were grown at a low density for 24h on the chamber slides (Nalge Nunc Int) and processed for immunofluorescence as described [28 (link)]. Cells were incubated with anti-MUC13 MAb (clone PPZ020) or anti-HER2 (catalog number A0485; DAKO), anti-p53 (catalog number 2527; Cell Signaling) followed by an incubation with species specific Alexa Fluor 488 (catalog number A11029; Invitrogen) or Alexa Fluor 568 (catalog number A11036; Invitrogen) secondary antibodies and were mounted in Fluoro Care Anti-Fade mounting medium (BioCare Medical, CA, USA). The cells were examined under a laser confocal microscope (Nikon Corporation).

Khan S., Ebeling M.C., Zaman M.S., Sikander M., Yallapu M.M., Chauhan N., Yacoubian A.M., Behrman S.W., Zafar N., Kumar D., Thompson P.A., Jaggi M, & Chauhan S.C. (2014). MicroRNA-145 targets MUC13 and suppresses growth and invasion of pancreatic cancer. Oncotarget, 5(17), 7599-7609.

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Immunofluorescence Staining of Treated Cells

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Cells were plated in 4 well chamber slides at 5×10⁴ cells per chamber. Cells were allowed to attach and reach 60% confluency (approximately 24 hr) before being treated with 10uM ORM for 18 hr. Cells were then processed for confocal microscopy as described earlier [16 (link)]. Briefly, following treatment cells were rinsed with 1× HEPES/Hank buffer, fixed and permeabilized with ice cold methanol, washed with 1× PBS and blocked with 10% normal goat serum in PBS. Cells were then incubated with the primary antibody (source listed in prior section) followed by a species specific Alexa Fluor® 488 secondary antibody (Invitrogen). After washing, cells were stained with DAPI and coverslips were mounted in FluoroCare Anti-Fade mounting medium (BioCare Medical). Confocal microscopy was performed with an Olympus Fluoview FV1000 confocal microscope (Olympus Corporation).

Maher D.M., Khan S., Nordquist J., Ebeling M.C., Bauer N.A., Kopel L., Singh M.M., Halaweish F., Bell M.C., Jaggi M, & Chauhan S.C. (2014). Ormeloxifene efficiently inhibits ovarian cancer growth. Cancer letters, 356(2 0 0), 606-612.

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