PO (156124) and febuxostat (FEB) (SML1285) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The primary antibodies used in the present study were purchased from Bioworld Technology (Nanjing, China) and were as follows: rabbit anti-collagen 3A1, rabbit anti-α-SMA, rabbit anti-fibronectin, rabbit anti-vimentin, rabbit anti-E-cadherin, rabbit anti-NLRP3, rabbit anti-ASC, rabbit anti-caspase-1, rabbit anti-IL-1β, rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and rabbit anti-TGFβ1 (BS7028, BS70000, BS90514, BS1491, BS1098, BS90948, BS6205, BS5641, AP0063, BS72410,BS91338). The assay kits for UA (ab65344), creatinine (ab65340), xanthine oxidase (XOD) (ab102522), IL-1β (ab197742), and IL-18 (ab216165) were purchased from Abcam (Cambridge, UK). The PrimeScript™ RT Master mix was obtained from Takara (Takara, Japan) and the FastStart Universal SYBR Green master mix was obtained from Roche (Basel, Switzerland).
Bs1491
Manufactured by Bioworld Technology
Sourced in China
The BS1491 is a piece of lab equipment designed for general laboratory use. It is a compact and versatile device that can perform various tasks. The core function of the BS1491 is to assist in the analysis and processing of samples within a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Ab216165, by Abcam (1 mentions)
Ab65344, by Abcam (1 mentions)
Ab65340, by Abcam (1 mentions)
Ab197742, by Abcam (1 mentions)
Ab102522, by Abcam (1 mentions)
Bs70000, by Bioworld Technology (1 mentions)
Ap0063, by Bioworld Technology (1 mentions)
Bs1098, by Bioworld Technology (1 mentions)
Primescript rt master mix, by Takara Bio (1 mentions)
Faststart universal sybr green master mix, by Roche (1 mentions)
Rabbit sp detection kit, by ZSGB-BIO (1 mentions)
Dab kit, by ZSGB-BIO (1 mentions)
2 protocols using bs1491
1
Febuxostat Attenuates Renal Fibrosis
2
Histological Characterization of Endometrial Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Eutopic/ectopic endometrial, and UC tissues were fixed in 4% formalin for paraffin section preparation. Each segment was sliced to a thickness of 3 microns. Hematoxylineosin staining was performed to identify the morphological structure of the segments. Additionally, these slides were stained with vimentin or cytokeratin7 (CK7) antibodies using immunohistochemistry to determine the cell types. The rabbit SP detection kit (ZSGB, Beijing, China) was utilized for IHC analysis following the manufacturer's instructions. Antigen retrieval was conducted using sodium citrate buffer (pH 6.0) in a pressure cooker. The slides were incubated overnight at 4°C with primary antibodies against vimentin (1:100 dilution; BS1491; Bio-world Technology) or CK7 (1:100 dilution; 22208-1-AP; Protein tech). PBS was used as a negative control. Chromogenic reaction was achieved using the DAB kit (ZSGB, Beijing, China).
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