Dimethyl sulfoxide (dmso)

Manufactured by Xilong

Sourced in China

Dimethyl sulfoxide (DMSO) is a versatile organic compound commonly used as a solvent in laboratory settings. It is a colorless, odorless, and polar liquid with a high boiling point. DMSO's primary function is to dissolve a wide range of organic and inorganic compounds, making it a valuable tool for various experimental and analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

10 protocols using dimethyl sulfoxide (dmso)

Synthesis and Characterization of Rare Earth Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nd(NO₃)₃·6H₂O and Gd(NO₃)₃·6H₂O were purchased from Beijing HWRK Co., LTD (Beijing, China). NH₄VO₃ was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dopamine hydrochloride and polyethylene glycol (PEG) were acquired from Sigma-Aldrich Co. (Saint Louis, MO, USA). Dimethyl sulfoxide (DMSO) was obtained from Xilong Chemical Co., Ltd. (Swatow, China). Bovine serum albumin (BSA) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). All chemicals were used as received.
TEM images of NPs were acquired from a FEI Tecnai G2 S-Twin operating at an acceleration voltage of 200 kV. The X-ray powder diffraction was obtained using a D8 Advanced diffractometer (Bruker) with Cu Kα radiation (λ = 0.15405 nm). The UV–VIS absorption was recorded on U-3100 spectrophotometer (Hitachi). The Zeta potential was measured using a Malvern instrument Zetasizer Nano. The Confocal laser scanning microscopy images was performed on a LSM FV 1000 instrument (Olympus). Thermogravimetry data were recorded on a Netzsch Thermal Analyzer (STA 409) in an ambient environment with a heating rate of 10 °C min⁻¹ from room temperature to 700 °C.

Deng K., Liu D., Wang Z., Zhou Z., Chen Q., Luo J., Zhang Y., Hou Z, & Lin J. (2022). Surface-Functionalized NdVO4:Gd3+ Nanoplates as Active Agents for Near-Infrared-Light-Triggered and Multimodal-Imaging-Guided Photothermal Therapy. Pharmaceutics, 14(6), 1217.

+ Open protocol

+ Expand

Synthesis and Characterization of Tellurium Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tellurium powder (99.999%) and ethyl thioglycolate were purchased from Jingchun Reagent Co., Ltd (Shanghai, China). Sodium borohydride and cadmium chloride were obtained from Yunzhan Chemical Co., Ltd (Shanghai, China). Hydrazine hydrate (80%), polyethylene glycol 4000 (PEG_4k) and dimethyl sulfoxide (DMSO) were supplied by Xilong Chemical Co., Ltd (Guangdong, China). Absolute ethyl alcohol was obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Acetic anhydride was purchased from Shentong Chemical Co., Ltd (Tianjin, China). Colorimetric kits for serum biochemical marker detection were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other materials and reagents were of analytical grade. Ultrapure water (≥18.2 MΩ) from a Milli-Q system (Millipore, USA) was used in all aqueous solutions.

Du Y., Zhong Y., Dong J., Qian C., Sun S., Gao L, & Yang D. (2019). The effect of PEG functionalization on the in vivo behavior and toxicity of CdTe quantum dots. RSC Advances, 9(22), 12218-12225.

+ Open protocol

+ Expand

Antioxidant and Anti-inflammatory Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

MEM and antibiotics (streptomycin and penicillin) were from HyClone, USA. Fetal bovine serum (FBS) was from Gemini Bio-products, USA. 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) and Xanthine oxidase (XOD, from bovine milk) were purchased from Sigma-Aldrich Chemicals Co. (St. Louis, MO, USA). 2,6-dihydroxyacetophenone (DHAP, 99%), 4-Hydroxyphenethyl alcohol (4-HPEA, 98%), and 4-Hydroxybenzaldehyde (HBA, 98%) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Xanthine and allopurinol were purchased from Shanghai Macklin Biochemical Co., Ltd. Dimethyl sulfoxide (DMSO) was obtained from Xilong Scientific Co., Ltd (Guangdong, China). All reagents were analytical grade. The deionized water was obtained from the Milli-Q Reagent Water System of Milliore Co. (Milford, MA).

Liu X., Wu D., Liu J., Li G., Zhang Z., Chen C., Zhang L, & Li J. (2022). Characterization of xanthine oxidase inhibitory activities of phenols from pickled radish with molecular simulation. Food Chemistry: X, 14, 100343.

+ Open protocol

+ Expand

Curcumin Loaded Phospholipid Nanoformulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Non-hydrogenated soybean phospholipid (SPC) Lipoid S100 (94% soybean phosphatidylcholine, 3% lysophosphatidylcholine, 0.1% phosphatidylethanolamine, 0.5% Nacyl-phosphatidylethanolamine, 0.1% phosphatidylinositol, 2% water, 0.2% ethanol) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Hydrogenated soybean phospholipid (HSPC, 97% 1,2-distearoyl-sn-glycero-3-phosphocholine) was purchased from Advanced Vehicle Technology Pharmaceutical Co., Ltd (Shanghai, China) . Curcumin (˃ 95%) was obtained from Hebei Food Additive Co., Ltd (Hebei, China) . Cholesterol was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) . The fluorescent probe, 1,6diphenyl-1,3,5-hexatriene (DPH, 98%), mucin from porcine stomach (M2378), pepsin from porcine gastric mucosa (P7125), pancreatin from porcine pancreas (P1750) and bile salts were purchased from Sigma-Aldrich (St. Louis MO, USA). Dimethyl sulfoxide (DMSO, 99%) was purchased from Xilong Scientific Co., Ltd (Guangzhou, China) . All other reagents used were analytical grade without further purification.

Tai K., Rappolt M., Mao L., Gao Y, & Yuan F. (2020). Stability and release performance of curcumin-loaded liposomes with varying content of hydrogenated phospholipids. Food chemistry, 326.

+ Open protocol

+ Expand

Evaluating PTEN's Anti-Proliferative Effect

Check if the same lab product or an alternative is used in the 5 most similar protocols

To evaluate the anti-proliferation ability of PTEN on AP-AR42J cells, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; AMERCO, USA) assay was performed. Briefly, cells in the logarithmic phase were seeded in each well of the 96-well plates at a density of 6 × 10³ cells. After incubation for different times (24, 48, or 72 h), 10 μL of MTT (5 mg mL⁻¹) was added and incubated with cells for 4 h to form the formazan. Thereafter, 150 μL of dimethyl sulfoxide (DMSO; XiLong Scientific) was supplemented and further incubation for 10 min was allowed. Finally, the absorbance was detected at 490 nm wavelength using a microplate reader (BIO-RAD, California, USA) and the cell viability and inhibition rate were evaluated by calculating the percentage of cell survival compared with the control.

Yan H., Jiang L., Zou H., Chen T., Liang H, & Tang L. (2019). PTEN suppresses the inflammation, viability, and motility of AP-AR42J cells by activating the Wnt/β-catenin pathway. RSC Advances, 9(10), 5460-5469.

+ Open protocol

+ Expand

Regulation of Insulin Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

MFA, described as 3,4-dimethoxy cinnamic acid (>98% purity), was purchased from Sigma Chemical Co. (St. Louis, MO, USA). MFA was dissolved in dimethyl sulfoxide (DMSO; Xilong Chemical Co., Ltd., Guangdong, China) for cell experiments in vitro and in starch paste for intragastric administration to rats in vivo. Absolute ethanol was purchased from Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China). The immunoprecipitation (IP) kit was obtained from Sangon Biotech Co., Ltd. (Shanghai, China). Recombinant human insulin injection was obtained from DONGBRD Pharmaceutical Co., Ltd. (Tonghua, China). Primary antibodies against IR, p-IR (Y1185), p-IRS1 (Y896), p-IRS2 (S731), PI3K-p85, PTEN, p-AKT2 (S474), GSK3β, p-GSK3β (Y216), FoxO1, p-FoxO1 (S256), SREBP1, p-GS (S641), and GS were purchased from Abcam Technology (Cambridge, UK). Primary antibodies against AKT1, p-AKT1 (Ser473), PEPCK, and AKT2 were obtained from Sangon Biotech. The primary antibody against PI3K-p110 was purchased from Cell Signaling Technology (Beverly, MA, USA). The primary antibody against G-6-Pase was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Primary antibody against FBPase was purchased from Roche (Mannheim, Germany). Primary antibody against GLUT2 and β-actin were obtained from ZSGB Biotech Co., Ltd. (Beijing, China). All other reagents were purchased from Sigma unless indicated otherwise.

Cheng Q., Li Y.W., Yang C.F., Zhong Y.J, & Li L. (2019). Ethanol-Induced Hepatic Insulin Resistance is Ameliorated by Methyl Ferulic Acid Through the PI3K/AKT Signaling Pathway. Frontiers in Pharmacology, 10, 949.

+ Open protocol

+ Expand

Microbubble-Mediated Simvastatin Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the products used in cell culture were obtained from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Antibodies, LATS1 small interfering (si)RNAs and LATS1 negative siRNAs were purchased from Abcam (Cambridge, UK). Simvastatin was purchased from Pfizer, Inc. (New York, NY, USA) and the solvent of Simvastatin was dimethyl sulfoxide (DMSO; Xilong Scientific Co., Ltd., Shenzhen, China). Microbubbles used in the present study were obtained from Definity (Lantheus Medical Imaging, Inc., North Billerica, MA, USA). The shell material, gas, mean size, concentration and microbubble half-life were phospholipid, C₃F8, 1.1–3.3 µm, 1.2×10¹⁰ bubbles/ml and 2–10 min, respectively. Based on previous studies, the concentration of microbubbles used in the present study was chosen as 20%; the ratio of microbubble solution: Cell suspension was 2:8 (32 (link),33 (link)).

Li H., Chen C, & Wang D. (2018). Low-frequency ultrasound and microbubbles combined with simvastatin promote the apoptosis of MCF-7 cells by affecting the LATS1/YAP/RHAMM pathway. Molecular Medicine Reports, 18(3), 2724-2732.

+ Open protocol

+ Expand

Anticancer Potential of EGCG in Ovarian and Lung Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ovarian cancer cell lines SKOV3, CAOV-3 and NIH-OVCAR-3 were obtained from the Kunming Cell Bank, Conservation Genetics, the Chinese Academy of Sciences. EGCG was purchased from Dalian Meilun Biotechnology Co., Ltd. The lung cancer cell line A549 and human retinal pigment epithelium (RPE) cell line were obtained from the Shanghai Cell Resource Center, the Chinese Academy of Biological Sciences. SKOV3 cells were maintained in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.), whereas the other cell lines were maintained in DMEM medium supplemented with 10% fetal bovine serum (both Invitrogen; Thermo Fisher Scientific, Inc.) and 1% antibiotic solution (100 U/ml penicillin and 100 µg/ml streptomycin) under humidified conditions with 5% CO₂ at 37°C. EGCG was dissolved to different concentrations (0, 5, 10, 20, 40 and 80 µg/ml) in RPMI-1640 medium; VO-Ohpic trihydrate (VO-Ohpic; Sigma-Aldrich; Merck KGaA) was dissolved in DMSO (Xilong Chemical Industry, China) and diluted to 0.1 µM with RPMI-1640 medium.

Qin J., Fu M., Wang J., Huang F., Liu H., Huangfu M., Yu D., Liu H., Li X., Guan X, & Chen X. (2020). PTEN/AKT/mTOR signaling mediates anticancer effects of epigallocatechin-3-gallate in ovarian cancer. Oncology Reports, 43(6), 1885-1896.

+ Open protocol

+ Expand

Purification of Botanical Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals, reagents, and solvents were of analytical grade and used directly without further purification as follows: thujone (TCI, Tokyo), terpineol (Santa Cruz Biotechnology, the Netherlands), pinene (TCI, Tokyo), limonene (Santa Cruz Biotechnology, the Netherlands), β-sitosterol (Sigma, USA), and dimethyl sulfoxide (DMSO) (Xilong Chemicals, China).

Al Tawalbeh D., Aburjai T., Al Balas Q, & Al Samydai A. (2022). In Silico and In Vitro Investigation of Anti Helicobacter Activity of Selected Phytochemicals. Journal of Pharmacy & Bioallied Sciences, 14(3), 132-139.

+ Open protocol

+ Expand

Synthesis of Iron Oxide Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Iron (III) chloride hexahydrate (FeCl₃·6H₂O), sodium acetate (NaAc) and ethylene glycol (EG) were purchased from Tianjin Fuyu Fine Chemical Reagent Corporation. Ammonium molybdate tetrahydrate ((NH₄)₆Mo₇O₂₄·4H₂O), thiourea (CH₄N₂S), polyethylene glycol (PEG 1000), silver nitrate (AgNO₃), glutaraldehyde (2.5%), 3, 3, 5, 5-Tetramethylbenzidine (TMB) and terephthalic acid (TPA) were obtained from Aladdin Reagent Co., Ltd (Shanghai, China). Methanol, hydrogen peroxide (H₂O₂) and dimethyl sulfoxide (DMSO) were provided by Xilong chemicals (China). All chemicals were used without any treatment. E. coli (ATCC25922) was bought from Shanghai Luwei Microbial SCI. &TECH. Co. Ltd.

Wei F., Cui X., Wang Z., Dong C., Li J, & Han X. (2020). Recoverable peroxidase-like Fe3O4@MoS2-Ag nanozyme with enhanced antibacterial ability. Chemical Engineering Journal, 408, 127240.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!