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Anti mda5 antibody

Manufactured by Cell Signaling Technology
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The Anti-MDA5 antibody is a laboratory tool used for the detection and identification of the MDA5 (melanoma differentiation-associated gene 5) protein. MDA5 is a pattern recognition receptor involved in the innate immune response against viral infections. The antibody can be used in various immunological techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the MDA5 protein.

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Lab products found in correlation

Mouse anti rabbit igg antibody, by Santa Cruz Biotechnology (2 mentions) Anti gapdh antibody, by Solarbio (2 mentions) Anti rig 1 antibody, by Cell Signaling Technology (2 mentions) Anti flag monoclonal antibody, by Bioss Antibodies (2 mentions) Anti anti β actin polyclonal antibody, by Solarbio (2 mentions) Anti oasl antibody, by Abcam (2 mentions) Anti rnase l antibody, by Santa Cruz Biotechnology (2 mentions) Horseradish peroxide conjugated rabbit anti mouse igg antibody, by Santa Cruz Biotechnology (2 mentions)

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Anti-MDA5 (16 citations)

2 protocols using anti mda5 antibody

1

Western Blot and Immunoprecipitation Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting and Immunoprecipitation were carried out as described previously [39] [40] [41] [42] . The primary antibodies were as follows: anti-FLAG monoclonal antibody (Bioss antibodies, Beijing, China, #bs-0879R), anti-Anti-β-actin Polyclonal Antibody (Solarbio, # K006153P), anti-GAPDH antibody (Solarbio, # K106389P), anti-OASL antibody (Abcam, Cambridge, MA. USA, # ab155422), anti-RNase L antibody (Santa Cruz Biotechnology, Dallas, TX, USA, # sc-74405), anti-RIG-I antibody (Cell Signaling Technology, Danvers, MA, USA, #3743), and anti-MDA5 antibody (Cell Signaling Technology, #5321). Secondary antibodies were horseradish peroxide-conjugated rabbit anti-mouse IgG antibody (Santa Cruz Biotechnology, #sc-358914), and mouse anti-rabbit IgG antibody (Santa Cruz Biotechnology, # sc-2357).
Zhao M., Sha H., Li H., Zhang H., Huang L, & Wang R. (2022). Interferon inducible porcine 2', 5'-oligoadenylate synthetase like-1 protein limits porcine reproductive and respiratory syndrome virus 2 infection via the MDA5-mediated interferon-signaling pathway. International immunopharmacology, 111.
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2

Western Blot and Immunoprecipitation Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting and Immunoprecipitation were carried out as described previously [39] [40] [41] [42] . The primary antibodies were as follows: anti-FLAG monoclonal antibody (Bioss antibodies, Beijing, China, #bs-0879R), anti-Anti-β-actin Polyclonal Antibody (Solarbio, # K006153P), anti-GAPDH antibody (Solarbio, # K106389P), anti-OASL antibody (Abcam, Cambridge, MA. USA, # ab155422), anti-RNase L antibody (Santa Cruz Biotechnology, Dallas, TX, USA, # sc-74405), anti-RIG-I antibody (Cell Signaling Technology, Danvers, MA, USA, #3743), and anti-MDA5 antibody (Cell Signaling Technology, #5321). Secondary antibodies were horseradish peroxide-conjugated rabbit anti-mouse IgG antibody (Santa Cruz Biotechnology, #sc-358914), and mouse anti-rabbit IgG antibody (Santa Cruz Biotechnology, # sc-2357).
Zhao M., Kong W., Kang Y., & Li H. (2020). Interferon Inducible Porcine 2′, 5′-oligoadenylate Synthetase-Like Protein Limits Porcine Reproductive and Respiratory Syndrome Virus Infection via the MDA5-Mediated Interferon-Signaling Pathway.
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