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St aia pack cort

Manufactured by Tosoh
Sourced in United States

The ST AIA-PACK CORT is a laboratory equipment product manufactured by Tosoh. It is designed for the measurement of cortisol levels in various samples. The device utilizes an immunoassay technique to perform the analysis.

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2 protocols using st aia pack cort

1

Investigating WBC Subsets and Cortisol Levels

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After an overnight fast, blood samples were collected in heparinized tubes between 8:00 a.m. and 9:00 a.m. and immediately transported to the laboratory. To determinate the WBC subset counts, the total number of WBC and leukocyte differential counts were calculated with a Coulter counter (CELL-DYN 4000; ABBOTT). Enumeration by flow cytometry with FACS count (Becton Dickinson) included the following cells: two types of T cell subsets CD4, CD8.
Serum levels of cortisol were measured using a competitive enzyme immunoassay which was performed entirely within the ST AIA-PACK CORT (Tosoh Bioscience).
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2

Measuring Morning Cortisol Levels

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Blood sampling for measurements of morning cortisol plasma levels was performed in all study participants at the beginning and the end of the two 4-week intervention periods, as per study protocol. All venous blood samples were collected between 0800 and 0900 hours, after overnight fasting (10 hours of overnight fasting without food or drink intake, except for water). All plasma cortisol measurements were carried out on an ΑΙΑ-600ΙΙ analyzer (TOSOH Bioscience, Inc., South San Francisco, CA, USA) using a competitive fluorescence enzyme immunoassay based on individual test cups, according to the manufacturer's instructions (ST AIA-PACK CorT, ToSoH Bioscience, Inc., South San Francisco, CA, USA; assay range: 0.2-60 µg/dL; intra-and inter-assay coefficient of variation <5.0%).
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