Mice were housed in metabolic cages and had free access to rodent chow and water. Food intake, water intake, and urine volume were measured daily. Urine was collected under mineral oil. Serum concentrations of Na + , K + , Ca 2+ , and HCO 3 -were measured in blood with an i-STAT R -1 analyzer and i-STAT EG7+ cartridges (Abbott Laboratories, Abbott Park, IL).
Urine chloride and sodium concentrations were measured using an EasyLyte Plus Na/K/Cl analyzer (Medica, Bedford, MA). Serum concentration of Na + , K + , Ca ++ , and HCO 3 -were measured using i-STAT R -1 analyzer with i-STAT EG7+ cartridges (Abbott Laboratories, Abbott Park, IL). Urine osmolality was measured with an Advanced Micro Osmometer (Advanced Instruments, Norwood, MA).
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