After the washes of the FISH procedure following probe hybridizations, cells were washed in PBS/0.1% Tween 20 (PBT) and incubated for 1 hour in PBT/2%BSA. Incubation with an anti-Pax6 rabbit polyclonal antibody (PRB-278P-0100, Covance; 1:1,000 dilution in PBT/2%BSA) was performed overnight at 4°C between coverlips and glass slides in a humid and dark chamber. Cells were then washed in PBT and incubated with a secondary antibody (Donkey anti-Rabbit Alexa-647, A31573, Invitrogen; 1:200 dilution in PBT/2%BSA) for 1 hour at room temperature between coverlips and glass slides in a humid and dark chamber. Cells were then washed in PBT, incubated in PBS with DAPI (final concentration at 1 μg/ml) and washed again at least three times for 5 min each in PBS. Coverslips were mounted on slides with Vectashield (CliniSciences) and sealed with nail polish.
Prb 278p 0100
Manufactured by Fortrea
The PRB-278P-0100 is a laboratory equipment product designed for scientific research and analysis. It is a precision instrument that provides accurate and reliable measurements. The core function of this product is to facilitate data collection and analysis in a laboratory setting. Detailed specifications and intended use information are not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using prb 278p 0100
1
Immunostaining of Pax6 in Cells
2
Immunostaining of Pax6 in Cells
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After the washes of the FISH procedure following probe hybridizations, cells were washed in PBS/0.1% Tween 20 (PBT) and incubated for 1 hour in PBT/2%BSA. Incubation with an anti-Pax6 rabbit polyclonal antibody (PRB-278P-0100, Covance; 1:1,000 dilution in PBT/2%BSA) was performed overnight at 4°C between coverlips and glass slides in a humid and dark chamber. Cells were then washed in PBT and incubated with a secondary antibody (Donkey anti-Rabbit Alexa-647, A31573, Invitrogen; 1:200 dilution in PBT/2%BSA) for 1 hour at room temperature between coverlips and glass slides in a humid and dark chamber. Cells were then washed in PBT, incubated in PBS with DAPI (final concentration at 1 μg/ml) and washed again at least three times for 5 min each in PBS. Coverslips were mounted on slides with Vectashield (CliniSciences) and sealed with nail polish.
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