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Methionine

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Methionine is an essential amino acid that plays a crucial role in various cellular processes. It functions as a building block for proteins and is involved in the synthesis of other important biomolecules, such as cysteine and taurine. Methionine is also a key component in the methylation cycle, which is essential for maintaining cellular health and function.

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Lab products found in correlation

Bovine serum albumin (bsa), by Sisco Research Laboratories (2 mentions) Cetyltrimethylammonium bromide, by Merck Group (2 mentions) Reduced glutathione, by Sisco Research Laboratories (2 mentions) Trolox, by Merck Group (2 mentions) Xylenol orange, by Merck Group (2 mentions) Hydroxylamine hydrochloride, by Sisco Research Laboratories (2 mentions) Riboflavin, by Sisco Research Laboratories (2 mentions) Dithiothreitol (dtt), by Sisco Research Laboratories (1 mentions) Nitro blue tetrazolium, by Sisco Research Laboratories (1 mentions) Antioxidant assay kit, by Cayman Chemical (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid, by Sisco Research Laboratories (1 mentions) Polyvinyl pyrrolidone, by Sisco Research Laboratories (1 mentions) Reduced nicotinamide adenine dinucleotide, by Sisco Research Laboratories (1 mentions) β endorphin, by Merck Group (1 mentions) Toluene diisocyanate, by Merck Group (1 mentions) Vanadium 3 chloride, by Merck Group (1 mentions) Dichlorofluorescin diacetate, by Merck Group (1 mentions) Prednisolone, by Merck Group (1 mentions) 2 4 dinitrophenylhydrazine, by Sisco Research Laboratories (1 mentions) Nicotinamide adenine dinucleotide, by Sisco Research Laboratories (1 mentions)

3 protocols using methionine

1

Antioxidant Assay Protocol

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Nitro blue tetrazolium (NBT), 2,4-dinitrophenyl hydrazine (DNPH), 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), dithiothreitol (DTT), 1-diphenyl-2-picrylhydrazyl (DPPH) radical, polyvinyl pyrrolidone, nicotinamide adenine dinucleotide phosphate reduced (NADPH), bovine serum albumin, and methionine were provided by Sisco Research Laboratories Pvt., Ltd. (Mumbai, India). Thiobarbituric acid (TBA) was purchased from Molychem (Mumbai, India). Antioxidant assay kit and Sephadex G-25 column were purchased from Cayman Chemical Company (MI, USA) and Sigma-Aldrich (MO, USA), respectively. Ethanol and α-tocopherol were obtained from Changshu Yangyuan Chemical (Jiangsu, China) and Acros Organics (NJ, USA), respectively. All the other chemicals employed were of standard analytical grade and provided by Thermo Fisher Scientific India Pvt., Ltd. (Mumbai, India).
Prathyusha I.V.S.N, & Chaitanya K.V. (2019). Effect of water stress on the physiological and biochemical responses of two different Coleus (Plectranthus) species. Biologia futura, 70(4).
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2

Biochemical Assays for Antioxidant and Enzyme Activity

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2ʹ,7ʹ-Dichlorofluorescin Diacetate (DCFDA), β-Endorphin, cetyl trimethyl ammonium bromide (CTAB), Toluene diisocyanate (TDI), Trolox, Vanadium chloride (III) and Xylenol orange were purchased from Sigma-Aldrich (USA). Bovine Serum Albumin (BSA), o-dianisidine dihydrochloride (ODD), methionine, Glutathione reduced (GSH), glutathione oxidised, nicotinamide adenine dinucleotide reduced (NADH), hydroxylamine hydrochloride and riboflavin were purchased from Sisco Research Laboratory (Mumbai, India). Sodium azide, 5, 5-Dithiobis-2-Nitrobenzoic acid (DTNB) and 1-Chloro-2,4-dinitrobenzene, (CDNB) were purchased from Loba chem. (India).
Pandey V., Yadav V., Singh R., Srivastava A, & S.u.b.h.a.s.h.i.n.i. (2023). β-Endorphin (an endogenous opioid) inhibits inflammation, oxidative stress and apoptosis via Nrf-2 in asthmatic murine model. Scientific Reports, 13, 12414.
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3

Evaluation of Oxidative Stress Markers

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2′,7′ − Dichlorofluorescin Diacetate (DCFDA), Vanadium chloride (III), Prednisolone (purity 99%), cetyltrimethylammonium bromide (CTAB), Xylenol orange and Trolox were purchased from Sigma − Aldrich (Burlington, USA). Bovine serum (BSA), o − dianisidine dihydrochloride (ODD), methionine, reduced Glutathione (GSH), oxidised Glutathione, nicotinamide adenine dinucleotide (NADH), hydroxylamine hydrochloride, 2,4 − dinitrophenylhydrazine and riboflavin were purchased from Sisco Research Laboratory (Mumbai, India). Sodium azide, 5, 5 − Dithiobis − 2 − Nitrobenzoic acid (DTNB) and 1 − chloro − 2,4 − di nitro benzene (CDNB) were purchased from Himedia (Maharashtra, India).
Srivastava A., S.u.b.h.a.s.h.i.n.i., Pandey V., Yadav V., Singh S, & Srivastava R. (2023). Potential of hydroethanolic leaf extract of Ocimum sanctum in ameliorating redox status and lung injury in COPD: an in vivo and in silico study. Scientific Reports, 13, 1131.
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