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2 protocols using nci h720

1

Culturing Human BP-NEN Cell Lines

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NCI-H727 and NCI-H720 cell lines, derived from human BP-NEN, were purchased from the American Type Culture Collection and were cultured in RPMI-1640 medium (Euroclone, Milan, Italy) supplemented with 10% fetal bovine serum, at 37°C in a humidified atmosphere with 5% CO2. Experiments were performed within the seventh passage.
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2

Culturing Human Lung Cancer Cell Lines

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Human bronchial epithelial cell line HBE (RRID:CVCL_0287) was a kind gift of Dr. Daniel Weiss (University of Vermont), and was maintained in BEGM media (Lonza,; Allendale, NJ). Human NSCLC lines NCI-H838 (RRID:CVCL_1594) and NCI-H1568 (RRID:CVCL_1476) were purchased from the NCI Division of Cancer Diagnosis and Treatment Tumor Repository (Operated by Charles River Laboratories, Inc.; Wilmington, MA), cell lines A549 (ATCC Cat# CCL-185, RRID:CVCL_0023), NCI-H292 (ATCC Cat# CRL-1848, RRID:CVCL_0455), NCI-H460 (ATCC Cat# HTB-177, RRID:CVCL_0459), and NCI-H1437 (ATCC Cat# CRL-5872, RRID:CVCL_1472) were obtained through the American Type Culture Collection (Manassas, VA), and lines NCI-H720 (RRID:CVCL_1583) and NCI-H1299 (RRID:CVCL_0060) were a kind gift of Dr. Jitesh Pratap (Rush University). All cancer cell lines were cultured in RPMI-1640 media supplemented with 10% fetal bovine serum (Cellgro-Corning Cat. No. 35-010-CV) and 1% penicillin/streptomycin (Hyclone-GE Cat. No. SV30079.01) and incubated in a 37 °C 5% CO2/95% air humidified incubator.
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