Hacat cell

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

HaCaT cells are a spontaneously immortalized human keratinocyte cell line. They are widely used in cell biology research as a model for human skin cells.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Lipofectamine, by GenePharma (2 mentions) Sirna constructs, by GenePharma (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Dm3000b microscope, by Leica (1 mentions) Millicell transwell culture inserts, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Penicillin, by Beyotime (1 mentions) Streptomycin, by Beyotime (1 mentions) Fetal bovine serum (fbs), by Wisent (1 mentions) Ws1 cells, by American Type Culture Collection (1 mentions) N n dimethylacetamide dmac, by Maclin Biochemical Technology (1 mentions) Tecnai g2 20 transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Nano tio2, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Penicillin streptomycin, by Cytiva (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Hacat cells, by Thermo Fisher Scientific (1 mentions) Rhtnf α, by R&D Systems (1 mentions)

23 protocols using hacat cell

Antiproliferative Potential of Toad Venom Proteins

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antiproliferative activity of the total protein of toad venom from HZ and LYG specimens against three selected cell lines was evaluated using an MTT assay, according to Mossman (1983). Briefly, HaCaT, MCF-7, and SGC-7901 cells (1 × 10⁴ cells/well) were seeded into 96-well plates and incubated with different concentrations of proteins extracted from toad venom (0.01, 0.1, 1, 10, and 100 µg/mL) for 48 h. The morphology of cells from each treatment was imaged using a Leica DM3000B microscope (Leica Microsystems GmbH, Frankfurt, Germany) [53 (link)].
Meanwhile, the cell growth rate in the presence and absence of the toad venom samples was compared, and the normalized growth rate inhibition (GR) was used to evaluate the effect of the samples on the cells [54 (link)]. Three cells (4 × 10⁶ cells/well) were seeded into 6-well plates and incubated with different concentrations of protein extracts (0.01, 0.1, 1, 10, 25, 50, 75, and 100 µg/mL) for 48 h. The cell count and death count were recorded when the extracts were present and absent, respectively. An online GR calculator (www.grcalculator.org) (accessed on 3 April 2023) was used for dose-response curves for GR value.
The HaCaT cell was provided by China Center for Type Culture Collection, CCTCC (Wuhan, China). The MCF-7 and SGC-7901 cells were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China).

Yang M., Huan W., Zhang G., Li J., Xia F., Durrani R., Zhao W., Lu J., Peng X, & Gao F. (2023). Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans. Molecules, 28(8), 3628.

+ Open protocol

+ Expand

Cellular Uptake of HA-Chol-NPs-NIC

Check if the same lab product or an alternative is used in the 5 most similar protocols

HaCaT cell was obtained from China Center for Type Culture Collection (Wuhan, People’s Republic of China). The cells were cultured in DMEM supplemented with FBS (10%, v/v), 100 IU/mL penicillin, and 100 μg/mL streptomycin in the humidified atmosphere of 95% with 5% CO₂ at 37°C. The medium was replenished every other day. The HaCaT cells were then cultured in a 15 mm² confocal dish (1×10⁵ cells/well) with DMEM medium. After cultured for 24 hours, the culture medium was removed and washed with phosphate buffer solution for three times. In all, 200 μL of HA–Chol–NPs–NIC, HA–Chol–NPs, NIC complex suspension, and aqueous suspension containing an equal amount of C6 (0.1%, w/v) were diluted by DMEM without FBS to 10 μg/mL and added to the dish, respectively. After culturing for 4 hours, the culture medium was removed and the cells were washed three times with phosphate buffer solution. Then the cells were fixed with 4% paraformaldehyde solution at ambient temperature for 15 minutes, and the nuclei of cells were labeled by DAPI. The cell uptake was evaluated using CLSM. The excitation and emission wavelengths of C6 were 430 nm and 485 nm, respectively.

Pan W., Qin M., Zhang G., Long Y., Ruan W., Pan J., Wu Z., Wan T., Wu C, & Xu Y. (2016). Combination of hydrotropic nicotinamide with nanoparticles for enhancing tacrolimus percutaneous delivery. International Journal of Nanomedicine, 11, 4037-4050.

+ Open protocol

+ Expand

Culturing HaCaT Human Keratinocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human keratinocytes cell line HaCaT cells (China Center for Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (Gibco, CA, USA). Cells have been authenticated by STR profiling.

Lian N., Chen Y., Chen S., Xiao T., Song C., Ke Y., Wei X., Gong C., Yu H., Gu H., Chen Q., Li M, & Chen X. (2022). Necroptosis-mediated HMGB1 secretion of keratinocytes as a key step for inflammation development in contact hypersensitivity. Cell Death Discovery, 8, 451.

+ Open protocol

+ Expand

Irradiated Keratinocytes Bystander Effect

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human immortalized keratinocytes HaCaT cells were obtained from China Center for Type Culture Collection (CCTCC, Wuhan, China). The human embryonic dermal fibroblasts WS1 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Both cell lines were grown in DMEM (high glucose, Sigma-Aldrich, St Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, Wisent, St-Bruno, Quebec, Canada), 100 U/ml streptomycin and 100 U/ml penicillin (both from Beyotime Institute of Biotechnology, China) at 37 ^°C in a humidified atmosphere of 95% air and 5% CO₂.
To study the factors in irradiated HaCaT cells that were involved in the bystander effect in unirradiated WS1 cells, a transwell insert co-culture system was utilized. 1.2 × 10⁵ HaCaT cells and 4 × 10⁴ WS1 cells were seeded on coverslips in 6-well plates (with a growth area of 9.6 cm²) and in companion Millicell^® transwell culture inserts (Millipore, MA, USA) (with a growth area of 5.7 cm²), respectively. The insert had a porous membrane with a pore size of 0.4 μm to allow the passage of molecules but not cells. Immediately after HaCaT being irradiated, the inserts with WS1 cells were put into the wells with HaCaT cells for 24 h. HaCaT and WS1 cells shared the same medium, but were separated from each other with a distance of 3 mm.

Yin X., Tian W., Wang L., Wang J., Zhang S., Cao J, & Yang H. (2015). Radiation quality-dependence of bystander effect in unirradiated fibroblasts is associated with TGF-β1-Smad2 pathway and miR-21 in irradiated keratinocytes. Scientific Reports, 5, 11373.

+ Open protocol

+ Expand

Emodin-loaded PVP/CA Nanofibers for MRSA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Emodin (purity, 98.6%) was purchased from Wei Ke Qi Biological Technology Co., Ltd. (Chengdu, China). Poly(vinyl pyrrolidone) (PVP, Mav = 1,300,000) and cellulose acetate (CA, acetyl content of 39.8%; MW = 30 kDa) were purchased from Dalian Meilun Biological Technology Co., Ltd. N,N-Dimethylacetamide (DMAC) and acetone were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Mannitol salt agar and Methicillin-resistant Staphylococcus aureus (MRSA) GDMCC 1.1263 were provided by Guangdong culture collection center (GDMCC 1.1263 = ATCC 43300). HaCaT cells were bought from China Center for Type Culture Collection (CCTCC, Wuhan, China).

Ye P., Wei S., Luo C., Wang Q., Li A, & Wei F. (2020). Long-Term Effect against Methicillin-Resistant Staphylococcus aureus of Emodin Released from Coaxial Electrospinning Nanofiber Membranes with a Biphasic Profile. Biomolecules, 10(3), 362.

+ Open protocol

+ Expand

HaCaT Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human keratinocyte cell line HaCaT cells (China Center for Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (Gibco, Invitrogen Corp., Carlsbad, CA, USA). HaCaT cells have been authenticated using STR profiling.

Chen Y., Lian N., Chen S., Xiao T., Ke Y., Zhang Y., Song C., Yang Y., Xu S., Gu H, & Chen X. (2022). GSDME deficiency leads to the aggravation of UVB-induced skin inflammation through enhancing recruitment and activation of neutrophils. Cell Death & Disease, 13(10), 841.

+ Open protocol

+ Expand

Characterization of Nano-TiO2 for Cytotoxicity Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nano-TiO 2 (P25, 25% rutile, and 75% anatase) with 21 nm was purchased from Degussa GmbH (Germany). The particle sizes and distribution of Nano-TiO 2 were measured by Tecnai G2 20 transmission electron microscope (FEI, Holland). Crystal structure was characterized by X'Pert Prox X-ray diffraction instrument (FEI). HaCaT cells were purchased from China Center for Type Culture Collection (China). Rhodamine 123, 2 0 ,7 0 -dichlorofluorescein diacetate (DCFH-DA) and 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma (St. Louis, MO). Modified Eagle medium (MEM), penicillin-streptomycin, and fetal bovine serum (FBS) were obtained from Hyclone Laboratories Inc. All other chemicals were of the highest grade that could be obtained commercially.

Xue C., Li X., Liu G, & Liu W. (2016). Evaluation of Mitochondrial Respiratory Chain on the Generation of Reactive Oxygen Species and Cytotoxicity in HaCaT Cells Induced by Nanosized Titanium Dioxide Under UVA Irradiation. International journal of toxicology, 35(6).

+ Open protocol

+ Expand

HaCaT Cells Culture and Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

HaCaT cells were purchased from the China Center for Type Culture Collection, Wuhan, China, and were cultured in DMEM containing 10%FBS in a 5% CO 2 incubator at 37°C. Lipofectamine was used to transfect cells with siRNA constructs (50 umol/L; GenePharma, Shanghai, China).

Xu Y., Lin Z., He L., Qu Y., Ouyang L., Han Y., Xu C., & Duan D. (2021). Platelet Rich Plasma-Derived Exosomal USP15 Promotes Cutaneous Wound Healing via Deubiquitinating EIF4A1.

+ Open protocol

+ Expand

Curcumin-Induced Differentiation of HaCaT Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immortalized human epidermal HaCaT cells (China Center for Type Culture Collection) were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum, 100 U/ml penicillin, and 100 mg/ml streptomycin (pH 7.2) at 37°C in air with 5% CO₂. Twenty-four hours after seeding, the HaCaT cells were maintained in RPMI-1640 with 7.5 mg/l curcumin for 72 h to induce differentiation. HaCaT cells cultured in RPMI-1640 medium were used as the control.

YANG H.B., SONG W., CHEN L.Y., LI Q.F., SHI S.L., KONG H.Y, & CHEN P. (2014). Differential expression and regulation of prohibitin during curcumin-induced apoptosis of immortalized human epidermal HaCaT cells. International Journal of Molecular Medicine, 33(3), 507-514.

+ Open protocol

+ Expand

HaCaT Cell Cytokine Stimulation and GSDME Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human keratinocyte cell line, HaCaT cells, were obtained from China Center for Type Culture Collection (Wuhan, China). HaCaT cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) (both from Gibco, CA, USA) in 5% CO₂ environment at 37 °C. HaCaT cells were treated with 10 ng/mL recombinant human (rh) IL-17A (R&D, Minneapolis, USA), 10 ng/mL rh OSM (R&D), 10 ng/mL rh TNF-α (R&D), 10 ng/mL rh IL22 (R&D), and 10 ng/mL rh IL1-α (R&D) in combination for 6, 12, 24, or 48 h. Transfection was performed according to previously described methods (45). For GSDME knockdown, HaCaT cells were transfected with pGLVH1/GFP + Puro lentivirus vector containing NC shRNA (shNC sequence: 5’-TTCTCCGAACGTGTCACGT-3’) or GSDME shRNA (shGSDME sequence: 5’-GCAGAAGTGTGTGATCTCTGA-3’) (GenePharma, Shanghai, China). Stable knockdown HaCaT cells were obtained and selected by puromycin incubation.

Long F., Wei X., Chen Y., Li M., Lian N., Yu S., Chen S., Yang Y., Li M., Gu H, & Chen X. (2024). Gasdermin E promotes translocation of p65 and c-jun into nucleus in keratinocytes for progression of psoriatic skin inflammation. Cell Death & Disease, 15(3), 180.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!