Mgi easy universal library conversion kit

A short-read sequence library was prepared using a PCR-free Swift 2S Turbo Flexible DNA Library Kit (Swift Sciences, Ann Arbor, MI, USA) and converted into a DNA nanoball sequencing library with an MGI Easy Universal Library Conversion Kit (MGI Tech). The library was sequenced on a DNBSEQ G400RS (MGI Tech) instrument in paired-end, 101-bp mode. The obtained reads were used to estimate the genome size with Jellyfish after removing low-quality bases (<10 quality value) with PRINSEQ, adaptor sequences (AGATCGGAAGAGC) with fastx_clipper in FASTX-Toolkit, and reads from organelle genomes (GenBank accession numbers: AP012207 and KY563267)¹⁵ (link)^,16 by read mapping with Bowtie2 on the reference sequences.

The MGIEasy Universal Library Conversion Kit (MGI Tech) was used to convert the libraries to DNBSEQ-compatible libraries. Sequencing was done by DNBSEQ-G400 (MGI Tech) with a DNBSEQ-G400RS High-throughput Sequencing Set (App-A FCL PE100) following the manufacturer's instructions. The resulting read lengths were as follows: Read 1-28 bp and Read 2-100 bp. Raw data were deposited at GEO with the accession code GSE243022.