Anti tcrβ antibody

Manufactured by BioLegend

Sourced in United States

Anti-TCRβ antibody is a laboratory reagent used to detect the T cell receptor beta chain (TCRβ) expressed on the surface of T cells. It can be used in various immunological techniques, such as flow cytometry and cell sorting, to identify and characterize T cell populations.

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2 protocols using anti tcrβ antibody

Comprehensive T cell immunophenotyping

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Anti-CD4 antibody (Clone: GK1.5 (FITC), SONY, Tokyo, Japan), anti-CD8a antibody (Clone: 53-6.7 (APC), SONY), anti-CD8b antibody (eBioH35-17.2 (PE), Invitrogen, CA, USA), anti-TCRβ antibody (Clone: H57-597 (FITC), SONY), anti-TCRβ antibody (Clone: H57-597 (APC/Cy7), BioLegend, CA, USA), anti-CD69 antibody (Clone: H1.2F3 (APC), BioLegend), anti-CD5 antibody (Clone: 53-7.3 (APC), BioLegend), anti-CD44 antibody (Clone: IM7 (Alexa Fluor 700), BioLegend), anti-CD62L antibody (Clone: MEL-14 (PE), BioLegend), anti-TCR Vβ5.1, 5.2 antibody (Clone: MR9-4 (PE), BioLegend), anti-TCR HY antibody (Clone: T3.70 (PE), eBioscience, CA, USA), anti-B220 antibody (Clone: RA3-6B2 (FITC), SONY), LEAF-purified anti-CD3ε antibody (Clone: 145-2C11, BioLegend), LEAF-purified anti-CD28 antibody (Clone: 37.51, BioLegend), Biotin anti-TCRβ antibody (Clone: H57-597, BioLegend) and Biotin anti-CD69 antibody (Clone: H1.2F3, BioLegend) were used in this study.

Hojo M.A., Masuda K., Hojo H., Nagahata Y., Yasuda K., Ohara D., Takeuchi Y., Hirota K., Suzuki Y., Kawamoto H, & Kawaoka S. (2019). Identification of a genomic enhancer that enforces proper apoptosis induction in thymic negative selection. Nature Communications, 10, 2603.

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Characterization of Immune Landscape in 4T1 Tumor Model

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The livers and bone marrow cells were harvested from sham and 4T1 cancer-bearing animals 14 days after transplantation. Red blood cells (RBCs) were lysed with RBC lysis buffer (BD Bioscience). The obtained cells were then stained with the following antibodies (BioLegend, CA, USA) for 30 min at 4 °C: anti-CD45 antibody (Clone: 30-F11), anti-CD19 antibody (Clone: 6D5), anti-TCRβ antibody (Clone: H57-597), anti-CD11b antibody (Clone: M1/70), anti-Ly6G antibody (Clone: 1A8), anti-FCεR1a antibody (Clone: MAR-1), anti-CD117 antibody (Clone: 2B8), anti-CD123 (Clone: 5B11), anti-CD49b antibody (Clone: DX5), anti-CD3 antibody (Clone: 17A2), anti-CD11c antibody (Clone: N418), anti-TER-119 antibody, or anti-CD34 antibody (Clone: HM34). Non-viable cells were stained with Propidium Iodide Solution (PI) and gated out. Data were acquired using FACS Aria (BD Bioscience) and analyzed using FlowJo software (v10.8.1, BD Biosciences). Gating strategy is shown in Supplementary Fig. 14.

Vandenbon A., Mizuno R., Konishi R., Onishi M., Masuda K., Kobayashi Y., Kawamoto H., Suzuki A., He C., Nakamura Y., Kawaguchi K., Toi M., Shimizu M., Tanaka Y., Suzuki Y, & Kawaoka S. (2023). Murine breast cancers disorganize the liver transcriptome in a zonated manner. Communications Biology, 6, 97.

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