Sw1990

The human pancreatic adenocarcinoma cell lines PANC1, AsPC1 and SW1990 were purchased from Shanghai Cell Bank of Chinese Academy of Sciences. All the cell lines were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Gibco, USA), 100 U/mL penicillin (Gibco, USA) and 100 mg/mL streptomycin (Gibco, USA) at 37 °C in a humidified atmosphere containing 5% CO₂.

Six human pancreatic cancer cell lines (BxPC-3, CFPAC-1, Colo-357, MiaPaCa-2, PANC-1, and SW1990) were purchased from the Shanghai Cell Bank (Shanghai, China). The normal human pancreatic ductal cell line, HPNE, was purchased from the American Type Culture Collection (ATCC, USA). Cell lines that stably overexpress YY1 (BxPC-3 and PANC-1), cell lines in which YY1 is knocked down, and control cell lines were prepared and cultured as previously described [20 (link)]. Human pancreatic cancer cell lines were cultured in DMEM supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 μg/mL). The HPNE cell line was cultured according to the recommendations of the ATCC.

Human PC cell lines (SW1990, BxPC-3 and) were obtained from the Shanghai Cell Bank (Shanghai, China). The SW1990, BxPC-3 and Panc-1 cell lines were cultured in Dulbecco's modied Eagle's medium (DMEM) or RPMI-1640 supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 0.1 mg/ml streptomycin, in an incubator at 37°C with 5% CO₂ atmosphere. Small interference RNA (siRNA) for the knockdown of lncRNA and negative control siRNA were constructed by GenePharma (Shanghai, China). Transfection was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The primers of siRNA target used in this study were as follows: siRNA-1: 5′-TGGTGGAGCCTAAAGACCTGAGGAT-3′; siRNA-2:5′GCTGCAGCAGAACACGAATATCAAT-3′; siRNA-3: 50027-GAGCCCGGCTCACATAATCAGCAAA-3′; si-NC: 5'-TGGGAGTCCAAACAGGTCGATGGA-3′. Complementary DNA encoding ENSG00000254041.1 was synthesized and subcloned into the pcDNA3.1(+) vector (Invitrogen) according to the manufacturer’s instructions. Transfections were performed using Lipofectamine 3000 and OPTI-MEM (Invitrogen) according to the manufacturer’s instructions. Cells transfected with scramble siRNA (siNC) or infected with blank pcDNA3.1(+) vector (blank vector) were used as negative controls.

HEK293T, HPDE pancreatic duct epithelial cell line and human PAAD cell lines (AsPC-1, BXPC3, CFPAC1, PANC-1, and SW1990) cells were obtained from the Shanghai Cell Bank. Cells were cultured in DMEM media supplemented with 10% fetal bovine serum (FBS), 100 units/mL penicillin and 100 μg/mL streptomycin.

Human normal pancreatic duct epithelial cell HPDE6-C7, and pancreatic cancer cell lines PANC-1, SW1990, AsPC-1 and BxPC-3 were purchased from Shanghai Cell Bank (Shanghai, China). DMEM medium (11,965,084, Gibco, Waltham, MA, USA) containing 10% fetal bovine serum (FBS, 10,099,141, Gibco) and 1% penicillin-streptomycin (15,070,063, Gibco) was used to culture cells in a humidified atmosphere at 37°C with 5% CO₂ [14 (link)].