Recombinant human follistatin was provided by Dr. Y. Eto (Central Research Laboratory, Ajinomoto, Kawasaki, Japan). Antibodies used in this study were as follows: goat anti-type I collagen antibody (1 : 100), goat anti-type III collagen antibody (1 : 100) (Southern BioTech, Birmingham, AL), mouse anti-α-SMA antibody (1 : 100) (Sigma, St. Louis, MO), mouse anti-CD68 antibody (1 : 100) (Abcam, Cambridge, UK), rabbit anti-inhibin βA antibody (1 : 100) (Thermo Fisher Scientific, Yokohama, Japan), rabbit anti-fibronectin antibody (1 : 100), goat anti-vimentin antibody (1 : 100) (Santa Cruz biotechnology, Inc., CA), rabbit anti-CD3 antibody (1 : 100) (Vector Labs, Burlingame, CA), Alexa Fluor 488 goat anti-mouse IgG (1 : 2000), and Alexa Fluor 488 goat anti-rabbit IgG (1 : 2000) (Invitrogen, Carlsbad, CA).
Goat anti type 3 collagen antibody
Manufactured by Southern Biotech
Goat anti-type III collagen antibody is a laboratory reagent used for the detection and quantification of type III collagen in various biological samples. It is a specific antibody raised in goats against type III collagen, a key structural protein found in the extracellular matrix of various tissues.
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Lab products found in correlation
Rabbit anti fibronectin antibody, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Mouse anti α sma antibody, by Merck Group (1 mentions)
Mouse anti cd68 antibody, by Abcam (1 mentions)
Goat anti vimentin antibody, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Rabbit antigoat horseradish peroxidase, by Agilent Technologies (1 mentions)
Sirius red f3b, by Merck Group (1 mentions)
Bx41 microscope, by Olympus (1 mentions)
2 protocols using goat anti type 3 collagen antibody
1
Immunofluorescence Analysis of Fibrotic Markers
2
Quantifying Kidney Fibrosis Using PSR and Collagen 3
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Picrosirius red (PSR) was performed on kidney and standard histological staining procedures using Sirius Red F3B (Sigma-Aldrich, St. Louis, Missouri). Ten cortical images were visualized at 100× magnification using an Olympus BX41 microscope (Olympus, Waltham, Massachusetts) with a linear polarizer. Imaging software (Image J) was used to calculate the percent area of polarization. Immunohistochemistry for collagen type 3 was performed following standard protocols using goat anti-type III collagen antibody (Cat # 1330-01, Southern Biotech, Birmingham, Alabama [1:100]), followed by rabbit antigoat horseradish peroxidase (Ref # P0449, Dako, Carpinteria, California [1:200]) and analyzed as described previously (29 (link)). Both kidneys were analyzed for PSR and collagen 3, and the average was used for analysis.
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