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Anti cd3 alexafluor 700 antibodies

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Anti-CD3 AlexaFluor 700 antibodies are a fluorescently-labeled monoclonal antibody that binds to the CD3 antigen expressed on T cells. The AlexaFluor 700 dye allows for detection of the antibody-bound cells using flow cytometry or other fluorescence-based methods.

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Lab products found in correlation

Anti ifn γ apc, by BD (2 mentions) Anti mip1β pe, by BD (2 mentions) Anti cd16 apc cy7, by BD (2 mentions) Anti cd107a pe cy5 stain, by BD (2 mentions) Perm a and b, by Thermo Fisher Scientific (2 mentions) 96 well elisa plate, by Thermo Fisher Scientific (2 mentions) Rosettesep isolation kit, by STEMCELL (2 mentions) Il 15, by STEMCELL (2 mentions) Brefeldin a, by Merck Group (2 mentions) Golgistop, by BD (2 mentions) Anti cd56 pe cy7, by BD (2 mentions)

2 protocols using anti cd3 alexafluor 700 antibodies

1

SARS-CoV-2 S Protein-Induced NK Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of NK-cell related responses, an ELISA-based assay was used. Therefore, 96-well ELISA plates (Thermo Fisher) were coated with SARS-CoV-2 S at 37°C for 2h. Plates were then washed and blocked with 5% BSA in PBS overnight at 4°C. NK cells were isolated from buffy coats from healthy donors (MGH blood donor center) using the RosetteSep isolation kit (Stem Cell Technologies) and NK cells were rested overnight supplemented with IL-15 (Stemcell). Serum samples were diluted 1:50 and incubated at 37°C for 2h on the ELISA plates. A staining cocktail of anti-CD107a-PE-Cy5 stain (BD), brefeldin A (Sigma), and GolgiStop (BD) was added to the NK cells and 5×104 NK cells per well were added and incubated for 5h at 37°C. NK cells were fixed and permeabilized using Perm A and B (Thermo Fisher) and surface markers were stained for with anti-CD16 APC-Cy7 (BD), anti-CD56 PE-Cy7 (BD) and anti-CD3 AlexaFluor 700 antibodies (BD). Intracellular staining included anti-IFNγ APC (BD) and anti-MIP-1β PE (BD). Acquisition occurred by flow cytometry iQue (Intellicyt), equipped with a robot arm (PAA). NK cells were defined as CD3-, CD16+ and CD56+. The ADNKA assay was performed in duplicate across two blood donors.
Mercado N.B., Zahn R., Wegmann F., Loos C., Chandrashekar A., Yu J., Liu J., Peter L., McMahan K., Tostanoski L.H., He X., Martinez D.R., Rutten L., Bos R., van Manen D., Vellinga J., Custers J., Langedijk J.P., Kwaks T., Bakkers M.J., Zuijdgeest D., Huber S.K., Atyeo C., Fischinger S., Burke J.S., Feldman J., Hauser B.M., Caradonna T.M., Bondzie E.A., Dagotto G., Gebre M.S., Hoffman E., Jacob-Dolan C., Kirilova M., Li Z., Lin Z., Mahrokhian S.H., Maxfield L.F., Nampanya F., Nityanandam R., Nkolola J.P., Patel S., Ventura J.D., Verrington K., Wan H., Pessaint L., Van Ry A., Blade K., Strasbaugh A., Cabus M., Brown R., Cook A., Zouantchangadou S., Teow E., Andersen H., Lewis M.G., Cai Y., Chen B., Schmidt A.G., Reeves R.K., Baric R.S., Lauffenburger D.A., Alter G., Stoffels P., Mammen M., Van Hoof J., Schuitemaker H, & Barouch D.H. (2020). Single-Shot Ad26 Vaccine Protects Against SARS-CoV-2 in Rhesus Macaques. Nature, 586(7830), 583-588.
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2

SARS-CoV-2 S Protein-Induced NK Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of NK-cell related responses, an ELISA-based assay was used. Therefore, 96-well ELISA plates (Thermo Fisher) were coated with SARS-CoV-2 S at 37°C for 2h. Plates were then washed and blocked with 5% BSA in PBS overnight at 4°C. NK cells were isolated from buffy coats from healthy donors (MGH blood donor center) using the RosetteSep isolation kit (Stem Cell Technologies) and NK cells were rested overnight supplemented with IL-15 (Stemcell). Serum samples were diluted 1:50 and incubated at 37°C for 2h on the ELISA plates. A staining cocktail of anti-CD107a-PE-Cy5 stain (BD), brefeldin A (Sigma), and GolgiStop (BD) was added to the NK cells and 5×104 NK cells per well were added and incubated for 5h at 37°C. NK cells were fixed and permeabilized using Perm A and B (Thermo Fisher) and surface markers were stained for with anti-CD16 APC-Cy7 (BD), anti-CD56 PE-Cy7 (BD) and anti-CD3 AlexaFluor 700 antibodies (BD). Intracellular staining included anti-IFNγ APC (BD) and anti-MIP-1β PE (BD). Acquisition occurred by flow cytometry iQue (Intellicyt), equipped with a robot arm (PAA). NK cells were defined as CD3-, CD16+ and CD56+. The ADNKA assay was performed in duplicate across two blood donors.
Mercado N.B., Zahn R., Wegmann F., Loos C., Chandrashekar A., Yu J., Liu J., Peter L., McMahan K., Tostanoski L.H., He X., Martinez D.R., Rutten L., Bos R., van Manen D., Vellinga J., Custers J., Langedijk J.P., Kwaks T., Bakkers M.J., Zuijdgeest D., Huber S.K., Atyeo C., Fischinger S., Burke J.S., Feldman J., Hauser B.M., Caradonna T.M., Bondzie E.A., Dagotto G., Gebre M.S., Hoffman E., Jacob-Dolan C., Kirilova M., Li Z., Lin Z., Mahrokhian S.H., Maxfield L.F., Nampanya F., Nityanandam R., Nkolola J.P., Patel S., Ventura J.D., Verrington K., Wan H., Pessaint L., Van Ry A., Blade K., Strasbaugh A., Cabus M., Brown R., Cook A., Zouantchangadou S., Teow E., Andersen H., Lewis M.G., Cai Y., Chen B., Schmidt A.G., Reeves R.K., Baric R.S., Lauffenburger D.A., Alter G., Stoffels P., Mammen M., Van Hoof J., Schuitemaker H, & Barouch D.H. (2020). Single-Shot Ad26 Vaccine Protects Against SARS-CoV-2 in Rhesus Macaques. Nature, 586(7830), 583-588.
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