Antibodies targeting

DMEM (Dulbecco's modified Eagle's medium)/Ham's F12, FBS and MitoSOX Red reagent were purchased from Invitrogen. AngII, Crystal Violet, NAC (N-acetyl-L-cysteine), antimycin A, ML171 (2-acetylphenothiazine), lucigenin, NADPH and CHX (cycloheximide) were purchased from Sigma Chemical Co. Chloroquine, MG132 and protease and phosphatase inhibitor cocktail were from Calbiochem. Anti-p-p47^phox (Ser³⁷⁰) antibody was obtained from Assay Biotechnology. Antibodies targeting p47^phox and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) were purchased from Cell Signaling Technology. Antibodies against PGC-1α, total Rac1 and p22^phox were from Santa Cruz Biotechnology. Antibodies targeting p40^phox, p67^phox and NOX1 were obtained from Abcam.
Male Sprague–Dawley rats 3–4 weeks of age were supplied by the Experimental Animal Center of Xi'an Jiaotong University, Xi'an, Shanxi, China. All animal experiments were approved by the Committee on the Ethics of Animal Experiments of Xi'an Jiaotong University.

Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Nacalai Tesque (Kyoto, Japan). Fetal bovine serum (FBS; #172012), and paclitaxel (T7402) were purchased from Sigma Aldrich (St. Louis, MO). Antibodies targeting E2F2 (ab70731) were purchased from Abcam (Cambridge, UK). Antibodies targeting RB (sc-50) were obtained from Santa Cruz Biotechnology (Dallas, TX), and those targeting phospho-RB (#9307) were obtained from New England Biolabs (Ipswich, MA). The cell cycle regulation antibody sampler kit (#9932), as well as the Antibodies targeting BMI1 (#2830) and CyclinD3 (#2936), were obtained from Cell Signaling (Danvers, MA). Antibodies targeting P16 (#10883-1-AP) and LaminB1 (#66095-1-IG) were obtained from Proteintech Group (Chicago, IL). TRIzol Reagent (#15596-018) was purchased from Life Technologies (Carlsbad, CA).