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Fitc labeled anti cd107a antibody

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The FITC-labeled anti-CD107a antibody is a fluorescently labeled monoclonal antibody that binds to the CD107a (LAMP-1) antigen. CD107a is a glycoprotein expressed on the surface of various cell types, including cytotoxic T cells and natural killer cells. This antibody can be used to detect and analyze CD107a-expressing cells using flow cytometry.

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Lab products found in correlation

Facsscan flow cytometer, by BD (1 mentions) Cytofix cytoperm, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Golgiplug golgistop, by BD (1 mentions)

Spelling variants of this product

Anti-CD107a (26 citations) Anti-CD107a antibody (14 citations) FITC anti-CD107a (6 citations) CD107a FITC (3 citations) Anti–CD107a-FITC antibody (2 citations)

2 protocols using fitc labeled anti cd107a antibody

1

Measuring NK Cell Degranulation

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Degranulation of NK cells was measured as previously described.24 (link) Briefly, activated NK cells were mixed with target cells at an effector-to-target ratio of 1:1 in the presence of FITC-labeled anti-CD107a antibody (BioLegend) and incubated for 1 or 2 h at 37 °C. Where indicated, target cells were first incubated with trastuzumab (0.105 mg/ml) before coculture with NK cells. Surface expression of CD107a was measured using a FACS scan flow cytometer (BD Biosciences), and data were analyzed using the FlowJo software program.
Zheng G., Guo Z., Li W., Xi W., Zuo B., Zhang R., Wen W., Yang A.G, & Jia L. (2021). Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduction and Targeted Therapy, 6, 236.
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2

Evaluating TILs Cytokine Secretion

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Purified expanded TILs were co-cultured with autologous KADA cell line (2:1 effector:target ratio; 105 TILs/well) in the presence of fluorescein isothiocyanate (FITC)-labeled anti-CD107a antibody (BioLegend). After two hours, cytokine secretion was blocked with GolgiPlug/GolgiStop (BD Biosciences) and further incubated for an additional four hours. Intracellular levels of IFN-γ and TNF-α (Table S2) were measured after cellular permeabilization with cytofix/cytoperm (BD Biosciences) in an LSRII flow cytometer (BD Biosciences).
Ligtenberg M.A., Pico de Coaña Y., Shmushkovich T., Yoshimoto Y., Truxova I., Yang Y., Betancur-Boissel M., Eliseev A.V., Wolfson A.D, & Kiessling R. (2018). Self-Delivering RNAi Targeting PD-1 Improves Tumor-Specific T Cell Functionality for Adoptive Cell Therapy of Malignant Melanoma. Molecular Therapy, 26(6), 1482-1493.
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