All procedures and animal studies were approved by the University of Nebraska Medical Center IACUC. The experimental groups consisted of 24 eight weeks-old male C57Bl/6 mice (The Jackson Laboratory, Bar Harbor, Maine) and 20 eight weeks-old male 129/SvEv mice (Taconic, Germantown, New York). Mice were anesthetized and underwent laparotomy. The abdominal aorta between the renal arteries and bifurcation of the iliac arteries was isolated from the surrounding retroperitoneal structures. The diameter of the aorta was measured using a Leica Application System (Leica Microsystems Inc, Buffalo Grove, IL). After baseline measurements, 0.25 M CaCl2 was applied to the aorta with care taken to avoid surrounding tissue. After 15 minutes the aorta was rinsed with 0.9 % sterile saline, the laparotomy incision was closed, and mice were returned to their cages after recovery. Six weeks later the mice underwent repeat laparotomy, dissection, and measurement of the aorta. Aortic diameter was measured at the point of maximal dilatation. The aorta tissue was collected for zymographic and Western blot analyses. For histological studies, the aorta was perfusion-fixed with 10% neutral buffered formalin. NaCl (0.9%) was substituted for CaCl2 in sham controls.
129 svev mice
Manufactured by Taconic Biosciences
Sourced in United States, Denmark
129/SvEv mice are an inbred mouse strain developed and maintained by Taconic Biosciences. These mice exhibit a genetic background that is commonly used in targeted mutagenesis and transgenic studies.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6n, by Taconic Biosciences (2 mentions)
Leica application system, by Leica Microsystems (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Mir 155 mice, by Jackson ImmunoResearch (1 mentions)
B6 sjl ptprc a pepc b boyj b6 sjl, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Taconic Biosciences (1 mentions)
Balb c mice, by Taconic Biosciences (1 mentions)
8 protocols using 129 svev mice
1
Mouse Aortic Dilatation Model
2
Mouse Strains Utilized for Research
3
Mouse Models for Preclinical Cancer Research
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Eight- to 12-week-old male 129Sv/Ev mice (for the NSCLC model) and female BALB/c mice (for the CT26 colon cancer model) were purchased from Taconic Biosciences (Rensselaer, NY, USA) and maintained by the Department of Veterinary Medicine and Surgery at The University of Texas MD Anderson Cancer Center. Protocols for animal use, treatment, and euthanasia were approved by the Institutional Animal Care and Use Committee of MD Anderson Cancer Center. All animal procedures were conducted in accordance with the ethical guidelines of the IACUC committee at MD Anderson Cancer Center (protocol# 00001018RN02).
4
Acute Kidney Injury Mouse Models
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From age-matched 8- to 10-week-old 129/SvEv mice (Taconic Biosciences), tissues were acquired from a sham mouse in which the abdomen was opened and sutured back and from mice that underwent IRI or CLP. In the IRI model (61 (link)), both renal pedicles were exposed and clamped for 22 minutes or 30 minutes through a midline incision and then released. Spatial transcriptomics, histopathology, CODEX, and immunofluorescence data were acquired from the 22-minute IRI model. scRNA-Seq data were acquired from the 30-minute IRI model. In the CLP model (59 (link)), the cecum was ligated and punctured with a 25-gauge needle. Kidneys were excised upon mouse euthanization 6 hours after each procedure and frozen in OCT compound. The presence or absence of AKI was assessed on the H&E histology image and in a consecutive periodic acid–Schiff-stained section. As expected, blood urea nitrogen and creatinine measurements were not elevated in either the IRI and CLP models at the 6-hour time point.
5
Unilateral Ureteric Obstruction in CD248-/- Mice
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129SvEv mice were purchased from Taconic, Denmark and maintained in 12-hour light/12-hour dark cycle with free access to food and water. All procedures were performed in accordance with UK Home Office guidelines. Transgenic mice lacking CD248-/- were generated and genotyped as previously described [18 (link)]. For all in vivo studies, 6 litter-matched male mice aged 8-10 weeks were used in each experimental group. Experiments were repeated 3 imes in independent experiments. Bone marrow chimeras were generated as previously described [19 (link)] using fetal liver cells expressing enhanced yellow fluorescent protein under the control of the Rosa26 promoter. Unilateral ureteric obstruction was performed by midline laparotomy; in brief, the left ureter was identified and ligated at 2 points. Sham-operated control mice underwent an identical procedure except that the left ureter was mobilized but not ligated. For all in vivo studies, 6 litter-matched male mice aged 8-10 weeks were used in each experimental group. Experiments were repeated 3 times in independent experiments.
6
Wild-Type Mice Behavioral Testing
7
Acute Kidney Injury in Mouse Models
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Animal experiments and protocols were approved by the Indiana University Animal Care and Use Committee. From agematched 8-10 week old 129/ SvEv mice (Taconic Biosciences, Albany, New York), tissues were acquired from a sham mouse in which the abdomen was opened and sutured back and mice which underwent ischemia-reperfusion injury (IRI) or cecal ligation and puncture (CLP). In the IRI model (59) , both renal pedicles were exposed and clamped for 22 minutes through a midline incision then released. In the CLP model (58) , the cecum was ligated and punctured with a 25 gauge needle. Kidneys were excised upon sacrifice 6 hours after each procedure and frozen in Optimal Cutting Temperature (OCT) compound. The presence or absence of acute kidney injury (AKI) was assessed on the H+E histology image and in a consecutive periodic acid-Schiff stained section. As expected, blood urea nitrogen and creatinine measurements are not elevated in either the IRI and CLP models at the 6 h timepoint.
8
Breeding and Housing of Transgenic Mice
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We used wild-type mice that were derived from an f1 cross between C57Bl/6N and 129Svev mice (Taconic). Breeding was conducted in the Hospital for Sick Children animal facility. Mice were housed in standard laboratory conditions with 4 mice per cage. The housing rooms were maintained on a 12 h light/dark cycle and behavioural testing was conducted during the light phase. All experiments were approved by the animal care committee at the Hospital for Sick Children and were conducted in accordance with the Canadian Council on Animal Care guidelines.
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