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Digiscan animal activity monitor

Manufactured by Omnitech Electronics
Sourced in United States

The Digiscan animal activity monitor is a device designed to measure and record the movement and activity levels of animals in a laboratory setting. It provides quantitative data on the locomotor activity of test subjects through digital tracking and analysis.

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9 protocols using digiscan animal activity monitor

1

Measuring Ambulatory Locomotor Activity

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Ambulatory locomotor activity was recorded for 30 min using automated cage activity meters equipped with photocells (Digiscan animal activity monitor, Omnitech Electronics, Columbus, Ohio) and measured as a number of beam breaks (results are presented in S4 Fig).
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2

Cocaine-Induced Locomotor Activity in Rats

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At PN50, control or Pb2+ exposed rats were placed into one of three experimental groups for the administration of saline, 5 mg kg−1 or 15 mg kg−1 cocaine–HCL. Before injection, all the rats were placed in an automated activity chamber (Digiscan Animal Activity Monitor, Omnitech Electronics) and locomotor activity was recorded for 60 min to allow for behavioral habituation. After 60 min had elapsed, the rats were administered a single intraperitoneal injection of saline, 5 mg kg−1 cocaine–HCL or 15 mg kg−1 cocaine–HCL before being placed back in the activity chamber for an additional 60 min to monitor locomotor activity. Total distance traveled was measured.
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3

Locomotor Activity Assessment in Rats

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Automated activity monitors (Digiscan animal activity monitor; Omnitech Electronics, Columbus OH) were used to assess locomotor activity. The monitors were equipped with two parallel rows of 16 sensors spaced 2.5 cm apart and were controlled by a computer using Micromax software (Omnitech Electronics). Rats that had previously undergone FST were subsequently examined in the locomotor activity assay and experiments were performed using a counterbalanced design to assign drug administration groups. For systemic administration, the drug was injected 20 min prior to the locomotor test, whereas VTA drug infusions were preformed immediately prior the test – consistent with the FST experimental design. Photobeam breaks were recorded, quantified and analyzed in 10 min bins.
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4

Pb2+ Exposure and Cocaine Response

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To determine if Pb2+ exposure increases the psychostimulant effects of an acute dose of cocaine at PN28, PN50, and PN120, naïve control animals or Pb2+-exposed male and female rats were placed into one of three experimental groups for the administration of vehicle-saline, 5 or 15 mg kg–1 cocaine-HCl. Prior to injection, all rats were placed in an automated activity chamber (Digiscan Animal Activity Monitor, Omnitech Electronics, Inc., Nova Scotia, Canada) and locomotor activity was recorded for 60 min to allow for behavioral habituation. After 60 min elapsed, rats were administered a single IP injection of vehicle-saline, 5 or 15 mg kg–1 cocaine-HCl before being placed back in the activity chamber for an additional 60 min to monitor locomotor activity. Total distance traveled (cm) was measured. Data are expressed as the percentage of the total distance traveled of the control-saline group within an age group. For subsequent experiments only the dose of cocaine that showed a significant locomotor activation in both experimental groups was used.
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5

Open Field Exploration in Mice

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All behavioral analysis was performed with male mice to avoid variations due to gender. Open field test. For the assessment of general locomotor and open field activity, animals were evaluated in an open field apparatus (Omnitech Electronics Digiscan animal activity monitor). Each mouse was placed in the arena (a 16 × 16 inch plexiglas chamber) for 5 min. Animal movements including locomotor activity (total distance traveled), freezing behavior (immobility), rearings, ambulations, and time spent in the center part of the field were recorded by infrared sensors mounted on to the walls of the chamber.
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6

Spontaneous Locomotor Activity in SCI Mice

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Spontaneous locomotor activity was assessed in naïve and SCI mice in a Digiscan Animal Activity Monitor system (Omnitech Electronics Model RXYZCM(8 (link)) TAO, Columbus, OH, USA), (26 (link), 31 (link)). Mice were placed in plexiglass chambers with photocell emitters and receptors spaced equally along the perimeter, creating a grid of infrared beams measuring total distance and vertical activity. Total distance was determined by centimeters travelled. Mice were tested in a darkened environment, and data were collected continuously for 10 min at the same time of day for each group.
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7

Locomotor Activity Monitoring

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Automated activity monitors (Digiscan animal activity monitor; Omnitech Electronics, Columbus OH) were used to assess locomotor activity. The monitors were equipped with two parallel rows of 16 sensors spaced 2.5 cm apart and were controlled by a computer using Micromax software (Omnitech Electronics). VTA drug infusions were performed immediately prior to the test – consistent with the FST, SPT, and EPM experimental design. Photobeam breaks were recorded, quantified and analyzed in 5 min bins.
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8

Assessing Exploratory Behavior in Rats

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In order to control for the potentially confounding effects of hypo- or hyperactivity on the other behavioral assays, exploratory activity in a novel open arena was evaluated over a 30 min session. Rats were placed in the center of the arena at the start of the testing session. Using methods similar to those previously described48 (link),54 (link), total distance traveled and time spent in the center of the arena were measured using one of two comparable automated systems: an opaque matte black arena (54.1 cm l × 54.1 cm w × 34.3 cm h) equipped with video tracking software (EthoVision XT 12; Noldus Information Technology, Wageningen, Netherlands) or the fully automated Digiscan Animal Activity Monitors with Integra software (Omnitech Electronics, Columbus, OH, USA). The testing room was illuminated to ~30 lux.
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9

Quantifying Open-Field Locomotor Activity

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Open-field activity measures naturally occurring behaviors exhibited when the animal explores and interacts with its surroundings. This test provides reliable data regarding gross motor function and specific activity related to psychological conditions such as anxiety-like behaviors [51] (link). The evaluation was conducted in a room isolated from acoustic interruptions. For assessment of locomotor behavior, we used the SuperFlex/Fusion system in the Digiscan Animal Activity Monitors (Omnitech Electronics, USA). Briefly, each rat was individually placed in a well-lit testing chamber constructed of Plexiglas (40 cm long × 40 cm wide × 30 cm height) for a 24-h observation period. Before recording started, some food pellets were dropped on the floor, and a plastic bottle filled with water was secured in one corner of the chamber. The animals were allowed to freely explore the box, and locomotion activity was captured by three-paired 16-photocell Superflex Sensors, which transmitted the location data to the Superflex Node. The apparatus was thoroughly cleaned after the removal of each animal. Data were processed using Fusion Software (version v5.3), and the following parameters were evaluated: (1) total distance traveled and (2) time spent in the center of the chamber [52] (link). The animals were taken to the third test the next day.
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