FA concentration was measured using either 2 μl of serum or 25 μl conditioned media with the NEFA kit (WAKO), using 75 μl Reagent A and 150 μl Reagent B. Absorbance was measured at 550 nm (reference 660 nm) using the manufacturer’s protocol. Free glycerol concentration in serum was measured by reacting 10 μl of serum with 190 μl Free Glycerol Reagent (Sigma) and absorbance was measured at 540 nm using the manufacturer’s protocol. Free glycerol concentration in cell culture media was measured by reacting 50 μl of conditioned media with 150 μl Free Glycerol Reagent reconstituted to 4/3x (Sigma) and absorbance was measured at 540 nm using the manufacturer’s protocol.
Free glycerol reagent reconstituted to 4 3x
Manufactured by Merck Group
The Free Glycerol Reagent reconstituted to 4/3x is a laboratory reagent. It is used to quantify the concentration of free glycerol in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using free glycerol reagent reconstituted to 4 3x
1
Quantifying Serum and Media Free Fatty Acids
2
Quantifying Serum and Media Free Fatty Acids
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