The SKBR3 breast cancer cells, obtained from ATCC, were maintained in DMEM (Sigma-Aldrich) with phenol red, supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin and 1% Glutamax (Life Technologies). Human umbilical vein endothelial cells (HUVECs) were obtained from Sigma-Aldrich Co Limited and cultured in endothelial growth medium (EGM) (Lonza, Basel, Switzerland), supplemented with 5% FBS (Lonza). MCF7 breast and LnCAP prostate cancer cells, both obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), were grown in DMEM and RPMI-1640 respectively, supplemented with 10% FBS, 1% penicillin/streptomycin and 1% Glutamax (Life Technologies). All cell lines were grown in a 37 ° C incubator with 5% CO2. Cells were switched to medium without serum the day before experiments.
Skbr3 breast cancer cells
Manufactured by American Type Culture Collection
SKBR3 cells are a human breast adenocarcinoma cell line derived from the pleural effusion of a female patient with metastatic breast cancer. They are adherent, epithelial-like cells commonly used in breast cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Mcf 7 breast, by American Type Culture Collection (1 mentions)
Mda mb 231 breast cancer cells, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Hct116 colon cancer cells, by American Type Culture Collection (1 mentions)
Hepg2 liver cancer cells, by American Type Culture Collection (1 mentions)
Skov 3 ovarian cancer cells, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Omega Scientific (1 mentions)
2 protocols using skbr3 breast cancer cells
1
Culturing Breast and Prostate Cancer Cell Lines
2
Cell Culture Protocols for Cancer Research
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