Recombinant mouse STI1 (His6-STI1) was generated and purified as described previously (Zanata et al. 2002 (link); Soares et al. 2013 (link); Maciejewski et al. 2016 (link)). Staurosporine (cat#PHZ1271; Invitrogen, Burlington, ON L7L 5Z1 Canada) was used as a positive control to induce apoptotic cell death (Chae et al. 2000 (link)). Methyllycaconitine citrate (MLA; Abcam, Cambridge, UK) was used to inhibit (Bertrand et al. 1997 (link)) and PNU 282987 (cat#ab120558; Abcam) to activate α7nAChR signaling (Hajos et al. 2005 (link)). LDN 193189 (cat#6053; Tocris, Burlington, Canada), a small molecule inhibitor of activin A receptor 1 (bone morphogenetic protein type 1 receptors), was used to inhibit ALK2 receptors (Cuny et al. 2008 (link)). BMP4 (cat#SRP3298; Sigma-Aldrich, Oakville, Ontario, Canada) dissolved in 10 mM citric acid was used as an agonist for ALK2 receptors (Chaikuad et al. 2012 (link)).
Methyllycaconitine citrate mla
Manufactured by Abcam
Sourced in United Kingdom, United States
Methyllycaconitine citrate (MLA) is a naturally occurring alkaloid compound. It functions as a selective antagonist for the nicotinic acetylcholine receptor.
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Lab products found in correlation
2 protocols using methyllycaconitine citrate mla
1
Purification and Application of Recombinant Mouse STI1
2
Mouse Model of Uterine Regeneration
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All experiments were performed in the in-house animal facility in accordance with the guidelines of the National Research Council’s Guide for the Care and Use of Laboratory Animals [34 ] and approved by the institutional experimental animals review board of Shanghai OB/GYN Hospital, Fudan University. A total of 60 female Balb/C mice, about 6–8 weeks old and about 18–21 g in bodyweight, were purchased from Shanghai LingChang Laboratory Animal Center (Shanghai, China) and used for this study. Among them, 20 were randomly selected as donors that contributed uterine fragments, and the remaining 40 were recipients.
The PNU-282987 and substance P (SP) were purchased from Sigma-Aldrich (St Louis, MO, USA), and methyllycaconitine citrate (MLA) from Abcam (Cambridge, UK). PNU-282987, MLA and SP were all dissolved in 0.9% sterile saline. As such, the control group used saline as a mock treatment.
The PNU-282987 and substance P (SP) were purchased from Sigma-Aldrich (St Louis, MO, USA), and methyllycaconitine citrate (MLA) from Abcam (Cambridge, UK). PNU-282987, MLA and SP were all dissolved in 0.9% sterile saline. As such, the control group used saline as a mock treatment.
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