Phospho eif2α ser51

Manufactured by Thermo Fisher Scientific

Phospho-eIF2α (Ser51) is a laboratory reagent used to detect and quantify the phosphorylation of the eukaryotic translation initiation factor 2 alpha (eIF2α) at serine 51. This phosphorylation event plays a role in the regulation of protein synthesis in response to various cellular stresses.

Automatically generated - may contain errors

Lab products found in correlation

Eif2α, by Cell Signaling Technology (1 mentions) Any kd sds page gels, by Bio-Rad (1 mentions) Hrp conjugated secondary antibody, by Cytiva (1 mentions) Isrib, by Merck Group (1 mentions)

2 protocols using phospho eif2α ser51

Western Blot Analysis of PERK, eIF2α, and Phospho-eIF2α

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with PBS and lysed in SDS-PAGE loading buffer (1% SDS, 62.5 mM Tris–HCl pH 6.8, 10% glycerol). Lysates were sonicated and loaded on Any-kD SDS-PAGE gels (BioRad). Proteins were transferred onto nitrocellulose and probed with primary antibodies diluted in Tris-buffered saline supplemented with 0.1% Tween 20 and 5% BSA. The following antibodies were used: PERK (D11A8) (1:1000), eIF2α (#9722; Cell Signaling technology) (1:1000), phospho-eIF2α (Ser51) (44728G; Invitrogen). An HRP-conjugated secondary antibody (Amersham) was employed to detect immune-reactive bands using enhanced chemiluminescence (SuperSignal, Thermo Scientific).

Sidrauski C., McGeachy A.M., Ingolia N.T, & Walter P. (2015). The small molecule ISRIB reverses the effects of eIF2α phosphorylation on translation and stress granule assembly. eLife, 4, e05033.

+ Open protocol

+ Expand

Molecular Signaling Pathway Investigation

Check if the same lab product or an alternative is used in the 5 most similar protocols

4OHT (cat. # H7904) and ICI (cat. # I4409) were obtained from Sigma, and ISRIB (cat.# 16,258) was purchased from Cayman. Primary antibodies were purchased for GFP (Proteintech, cat. # 66,002–1) and phospho-eIF2α (Ser51) (Invitrogen, cat.# 701,268). Secondary antibodies, Alexa Fluor 594 (cat. # A21207) and Alexa Fluor 488 (cat.# A21202), were purchased from Invitrogen.

Semina S.E., Pal P., Kansara N.S., Huggins R.J., Alarid E.T., Greene G.L, & Frasor J. (2022). Selective pressure of endocrine therapy activates the integrated stress response through NFκB signaling in a subpopulation of ER positive breast cancer cells. Breast Cancer Research : BCR, 24, 19.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!