Mouse anti horse cd44 clone cvs18

Immunophenotypic analysis of BM-MSCs, AT-MSCs and UC-MSCs was performed at P3 with the FACS Calibur flow cytometer (BD, Franklin Lakes, NJ, USA) using the following antibodies: mouse anti-rat CD90-FITC (clone OX7, Caltag Laboratories, Burlingame, California, USA), mouse anti-human CD34-FITC (clone 581, BD, USA), mouse anti-human CD105-FITC (clone SN6, Abcam, San Francisco, California, USA), mouse anti-horse CD44 (clone CVS18, AbD Serotec, Kidlington, Oxfordshire, UK) and mouse anti-horse MHC class II monomorphic (clone CVS20, AbD Serotec, UK). For the unconjugated primary markers, the secondary antibody goat anti-mouse IgG-FITC (AbD Serotec, UK) was used. The protocols used were those described by the manufacturers.

The immunophenotypic analysis of the BM-MSCs was performed on the primary cultured cells, using a FACSCalibur cytometer (Becton Dickinson and Company, USA) using monoclonal mouse anti-rat CD90 (clone OX7, 1:100, Caltag Laboratories, USA) and mouse anti-human CD34 (clone 581/CD34, 1:50, Becton Dickinson and Company, USA) antibodies labeled with fluorescein isothiocyanate (FITC) and a mouse anti-horse CD44 (clone CVS18, 1:100, AbD Serotec, USA) antibody that was detected using an FITC-conjugated goat anti-mouse secondary antibody (1:200, Molecular Probes, USA). During the analysis, 10,000 events were recorded.