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Anti cd107α

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Anti-CD107α is a monoclonal antibody that binds to the CD107α (LAMP-1) cell surface antigen. CD107α is a lysosome-associated membrane protein that is expressed on the surface of cells during degranulation, a process involved in the release of cytotoxic granules. Anti-CD107α can be used to detect and quantify degranulation in various cell types, such as T cells and natural killer cells.

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Lab products found in correlation

Anti cd107b, by BD (4 mentions) Tapi 2, by Avantor (4 mentions) Monensin and brefeldin a, by Thermo Fisher Scientific (1 mentions)

4 protocols using anti cd107α

1

Multiparametric CyTOF Analysis of PBMCs

Cited 1 time
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PBMCs were stimulated for 3h with 150ng/ml PMA + 1µM ionomycin in RPMI
plus brefeldin A and monensin (eBioscience), 2.5µg/ml anti-CD107α,
1.25µg/ml anti-CD107b (BD Bioscience), and 10µM TAPI-2 (VWR
International). Following stimulation, cells were resuspended in cytometry buffer (PBS +
0.05% sodium azide + 2mM EDTA + 2% fetal calf serum) and stained with
isotope-tagged antibodies before being acquisition on the CyTOF. For the detailed protocol
see (Newell et al., 2012 (link)) and Supplemental
Experimental Procedures.
Fergusson J.R., Smith K.E., Fleming V.M., Rajoriya N., Newell E.W., Simmons R., Marchi E., Björkander S., Kang Y.H., Swadling L., Kurioka A., Sahgal N., Lockstone H., Baban D., Freeman G.J., Sverremark-Ekström E., Davis M.M., Davenport M.P., Venturi V., Ussher J.E., Willberg C.B, & Klenerman P. (2014). CD161 Defines a Transcriptional and Functional Phenotype across Distinct Human T Cell Lineages. Cell Reports, 9(3), 1075-1088.
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2

PMA and Ionomycin Stimulation of PBMCs

Cited 1 time
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PBMCs were stimulated for 3 hr with 150 ng/ml PMA + 1 μM ionomycin in RPMI plus brefeldin A and monensin (eBioscience), 2.5 μg/ml anti-CD107α, 1.25 μg/ml anti-CD107b (BD Bioscience), and 10 μM TAPI-2 (VWR International). Following stimulation, cells were resuspended in cytometry buffer (PBS + 0.05% sodium azide + 2 mM EDTA + 2% fetal calf serum) and stained with isotope-tagged antibodies before being acquired on the CyTOF. For the detailed protocol, see Newell et al. (2012 (link)) and the Supplemental Experimental Procedures.
Fergusson J.R., Smith K.E., Fleming V.M., Rajoriya N., Newell E.W., Simmons R., Marchi E., Björkander S., Kang Y.H., Swadling L., Kurioka A., Sahgal N., Lockstone H., Baban D., Freeman G.J., Sverremark-Ekström E., Davis M.M., Davenport M.P., Venturi V., Ussher J.E., Willberg C.B, & Klenerman P. (2014). CD161 Defines a Transcriptional and Functional Phenotype across Distinct Human T Cell Lineages. Cell Reports, 9(3), 1075-1088.
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3

CyTOF Profiling of Stimulated PBMCs

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PBMCs were stimulated for as above in the presence of 2.5µg/ml anti-CD107α, 1.25µg/ml anti-CD107b (BD Bioscience) and 10µM TAPI-2 (VWR International, West Sussex, UK). Following stimulation, cells were resuspended in cytometry buffer (PBS + 0.05% sodium azide + 2mM EDTA + 2% fetal calf serum) and stained with isotope-tagged antibodies before being acquisition on the CyTOF as previously described19 (link).
Fergusson J.R., Hühn M.H., Swadling L., Walker L.J., Kurioka A., Llibre A., Bertoletti A., Holländer G., Newell E.W., Davis M.M., Sverremark-Ekström E., Powrie F., Capone S., Folgori A., Barnes E., Willberg C.B., Ussher J.E, & Klenerman P. (2015). CD161int CD8+ T cells: a novel population of highly functional, memory CD8+ T cells enriched within the gut. Mucosal immunology, 9(2), 401-413.
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4

CyTOF Profiling of Stimulated PBMCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were stimulated for as above in the presence of 2.5µg/ml anti-CD107α, 1.25µg/ml anti-CD107b (BD Bioscience) and 10µM TAPI-2 (VWR International, West Sussex, UK). Following stimulation, cells were resuspended in cytometry buffer (PBS + 0.05% sodium azide + 2mM EDTA + 2% fetal calf serum) and stained with isotope-tagged antibodies before being acquisition on the CyTOF as previously described19 (link).
Fergusson J.R., Hühn M.H., Swadling L., Walker L.J., Kurioka A., Llibre A., Bertoletti A., Holländer G., Newell E.W., Davis M.M., Sverremark-Ekström E., Powrie F., Capone S., Folgori A., Barnes E., Willberg C.B., Ussher J.E, & Klenerman P. (2015). CD161int CD8+ T cells: a novel population of highly functional, memory CD8+ T cells enriched within the gut. Mucosal immunology, 9(2), 401-413.
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