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Iris digital cell imaging system

Manufactured by Logos Biosystems
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Sourced in Cameroon, United States

The IRiS™ Digital Cell Imaging System is a compact and automated microscope for live-cell imaging. It captures high-quality digital images and time-lapse videos of cells in culture.

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Lab products found in correlation

Click it edu imaging kit, by Thermo Fisher Scientific (3 mentions) Lab tek 2 cc2 chamber slide system 4 well, by Thermo Fisher Scientific (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) In situ cell death detection kit, by Roche (2 mentions) Alexa fluor 488 imaging kit, by Thermo Fisher Scientific (2 mentions) Nylon membrane filter, by Merck Group (2 mentions) S 4800, by Hitachi (2 mentions) S 4800 scanning electron microscope, by Hitachi (2 mentions) Carboxyfluorescein diacetate succinimidyl ester, by Thermo Fisher Scientific (2 mentions) 96 well polystyrene plate, by SPL Life Sciences (2 mentions) Eclipse ti, by Nikon (2 mentions) Hcp 2, by Hitachi (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Mitotracker, by Cell Signaling Technology (1 mentions) Mito sox, by Logos Biosystems (1 mentions) Alexa flour 488 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Gold seal cover glass, by Electron Microscopy Sciences (1 mentions)

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IRiS Digital imaging system (5 citations) Digital Cell Imaging System (4 citations)

65 protocols using iris digital cell imaging system

1

Mito-Ca2+ Levels in Oocyte Maturation

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To evaluate mito-Ca2+ levels in maturated and fertilized oocytes, we performed fluorescence staining using Rhod-2 AM (ab142780; Abcam, Cambridge, MA, USA) as a specific
mitochondria calcium detection dye. To identify Rhod-2 fluorescence expression in mature oocytes (44 h after IVM) and fertilized oocytes (3 h or 6 h after IVF), oocytes were washed
with 0.1% PBS-polyvinyl alcohol (PVA) and incubated in the dark at 38.5ºC and 5% CO2 for 15 min in IVF medium supplemented with 100 μM Rhod-2 dye. After Rhod2 treatment,
oocytes were washed three times in 0.1% PVA-PBS (w/v) and Rhod-2 fluorescence was measured using an iRiSTM digital cell imaging system (Logos Biosystems, Anyang, Korea).
Each sample was washed three times in 0.1% PVA-PBS. Washed presumptive zygotes were cultured in 50 μl of IVC medium mixed with 5 μM Mito-SOX (red fluorescence; Life Technologies,
Carlsbad, CA, USA) under mineral oil at 37°C for 30 min. To confirm the mitochondria localization, embryos cultured in 50 μl of IVF medium were mixed with 4 μg/ml Mito-Tracker
(Cell Signaling Technology, Danvers, MA, USA) at 38.5ºC for 20 min. Each sample was washed three times in 0.1% PVA-PBS (Supplementary Fig. 5: online only). Mito-Tracker (green) and Mito-SOX (red)-positive cytoplasm in cells were detected using an iRiSTM digital cell imaging
system (Logos Biosystems).
JEGAL H.G., PARK H.J., KIM J.W., YANG S.G., KIM M.J, & KOO D.B. (2020). Ruthenium red improves blastocyst developmental competence by regulating mitochondrial Ca2+ and mitochondrial functions in fertilized porcine oocytes in vitro. The Journal of Reproduction and Development, 66(4), 377-386.
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2

Quantifying Candida albicans Cell Aggregation

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Cell aggregation was analyzed as previously described (Zelante et al., 2012 (link)). Briefly, C. albicans cells were inoculated into 2 ml of PDB medium or RPMI-1640 medium at density of 105 CFU/ml in 14 ml test tubes with or without 6-gingerol or 6-shogaol and incubated at 37°C for 24 h with shaking at 250 rpm. Cell cultures (2 ml) were then transferred into glass-bottom dishes and observed. Aggregated cells were visualized in bright field using the iRiSTM Digital Cell Imaging System (Logos Bio Systems, Korea) at a magnification of 4x. At least, four independent experiments were conducted.
Lee J.H., Kim Y.G., Choi P., Ham J., Park J.G, & Lee J. (2018). Antibiofilm and Antivirulence Activities of 6-Gingerol and 6-Shogaol Against Candida albicans Due to Hyphal Inhibition. Frontiers in Cellular and Infection Microbiology, 8, 299.
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3

C. elegans Killing Assay with Curcumin

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The C. elegans killing assay used was a modification of a previously described protocol (Beceiro et al., 2014 (link)). Briefly, non-infected nematodes (∼20–30) [fer-15(b26);fem-1(hc17)] were pipetted into 96-well plate containing M9 buffer and overnight curcumin (50 μg/ml) treated and untreated with A. baumannii and/or C. albicans cells. As a second dose curcumin was added to respective wells to make final concentration 50 μg/ml (total volume 300 μl). Nematodes were incubated at 25°C and viabilities were determined as previously described (Rajsekharan et al., 2018 (link)), by exposing them to LED or UV LED lights for 10–30 s using an iRiSTM Digital Cell Imaging System (Logos BioSystems, South Korea). Three independent experiments (n = ∼20–30) were conducted.
Raorane C.J., Lee J.H., Kim Y.G., Rajasekharan S.K., García-Contreras R, & Lee J. (2019). Antibiofilm and Antivirulence Efficacies of Flavonoids and Curcumin Against Acinetobacter baumannii. Frontiers in Microbiology, 10, 990.
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4

Alizarin Effects on Candida Filamentation

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Cell aggregation and filamentous growth were investigated as previously described [18 (link)], C. albicans cells were inoculated in 1.5 mL of hyphae promoting RPMI-1640 medium supplemented with 10% fetal bovine serum at a cell density of 105 CFU/mL in 1.6 mL tubes with or without alizarin (2, 10, or 50 μg/mL) and incubated under anaerobic conditions at 37 °C for 24 h. After incubation, aggregated cells and filamentous growths were analyzed using the iRiSTM Digital Cell Imaging System (Logos Biosystems, Anyang, Korea) under bright field at 4× and 10× magnifications. At least four independent experiments were performed.
Lee J.H., Kim Y.G., Park S., Hu L, & Lee J. (2022). Phytopigment Alizarin Inhibits Multispecies Biofilm Development by Cutibacterium acnes, Staphylococcus aureus, and Candida albicans. Pharmaceutics, 14(5), 1047.
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5

Filament Induction in Candida albicans

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To investigate filament induction on solid media, C. albicans cells from glycerol stock, were plated on PDA agar plates including 10% fetal bovine serum supplemented with DMSO [less than 0.1% (vol/vol)] or methylindoles at 0.1 mM. Plates were then incubated for 6 days at 37°C, during which fungal colony morphologies were photographed after 2 days of interval using an iRiSTM Digital Cell Imaging System (Logos Biosystems, South Korea).
Lee J.H., Kim Y.G., Gupta V.K., Manoharan R.K, & Lee J. (2018). Suppression of Fluconazole Resistant Candida albicans Biofilm Formation and Filamentation by Methylindole Derivatives. Frontiers in Microbiology, 9, 2641.
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6

Visualizing Hyphae Inhibition by GO-AZ

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To visualize the impact of GO-AZ on pathogenic hyphae growth, we performed hyphae inhibition assay as described previously [27 (link)]. Briefly, overnight-grown yeast cell suspension in RPMI 1640 (Invitrogen, USA) medium buffered with HEPES (pH 7.3) was incubated with AZ, GO, and GO-AZ at 37 °C for 24 h with agitation (200 r/min). Fungal cells were observed under iRiSTM digital cell imaging system (Logos Bio Systems, Korea).
Ramasamy M., Nanda S.S., Lee J.H, & Lee J. (2020). Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of Candida albicans Biofilms. Biomolecules, 10(4), 565.
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7

Longevity Assay in C. elegans

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C. elegans assays were performed as reported previously [19 (link)]. In brief, noninfected C. elegans fer-15; fem-1 worms (n = ~ 20–30) were pipetted into single wells of a 96-well plate suspended in M9 buffer. Nervonic, oleic, or myristoleic acid (50 µg/mL) was then added to a final volume of 300 µL. Nematodes were incubated for 4 days at 25 °C, and viabilities were determined using an iRiSTM Digital Cell Imaging System (Logos BioSystems, Anyang, Korea) by exposing worms to LED or UV LED light for 10–30 s.
Khadke S.K., Lee J.H., Kim Y.G., Raj V, & Lee J. (2021). Assessment of Antibiofilm Potencies of Nervonic and Oleic Acid against Acinetobacter baumannii Using In Vitro and Computational Approaches. Biomedicines, 9(9), 1133.
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8

Hyphal Growth and Cell Aggregation in C. albicans

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To investigate hyphal growth and cell aggregation, C. albicans cells were inoculated into 2 ml of PDB medium at a density of 105 CFU ml−1 in 14 ml test tubes with or without fatty acids (2 μg ml−1) or farnesol (100 μg ml−1) and incubated at 37°C for 24 h without shaking. After incubation for 24 h, aggregated cells and hyphal growths were visualized in bright field using the iRiSTM Digital Cell Imaging System (Logos Bio Systems) at magnifications of 4x and 10x. At least, four independent experiments were conducted.
Lee J., Kim Y., Khadke S.K, & Lee J. (2020). Antibiofilm and antifungal activities of medium‐chain fatty acids against Candida albicans via mimicking of the quorum‐sensing molecule farnesol. Microbial Biotechnology, 14(4), 1353-1366.
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9

Immunocytochemistry in Chamber Slides

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Cells were cultured in the Lab-Tek II CC2 Chamber Slide System 4-well (Thermo Fisher Scientific). After the indicated treatment, the cells were fixed and permeabilized in cold methanol for 10 min at −20 °C. Then, the slides were washed with 1× DPBS for 10 min and blocked with Odyssey Blocking Buffer (LI-COR Biosciences, Lincoln, NE, USA) for 1 h. The slides were incubated in Odyssey Blocking Buffer with 1:100 diluted primary antibodies at 4 °C for 12 h. Images were captured and analyzed using an iRiSTM Digital Cell Imaging System (Logos Biosystems, Annandale, VA, USA).
Patil G., Xu L., Wu Y., Song K., Hao W., Hua F., Wang L, & Li S. (2020). TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Vesicular Stomatitis Virus Infection. Viruses, 12(2), 131.
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10

Herring Oil Effect on S. aureus Virulence

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To investigate the effects of herring oil and unsaturated fatty acids on the virulence of S. aureus MSSA 6538, we used a nematode survival assay as previously described (Kim et al., 2016 (link)) with slight modification. In brief, S. aureus cells were incubated with or without herring oil, DHA, or EPA (2, 5, or 20 μg/ml) at 37°C for 24 h and synchronized adult C. elegans fer-15;fem-1 nematodes were added into single wells of 96-well plate containing cultivated S. aureus cells. Approximately, 30 nematodes were allowed to feed on the cultured S. aureus MSSA 6538 at 25°C for 1 day.
For the cytotoxicity assay, 110 ± 10 nematodes were added into single well of 96-well plates containing M9 buffer and solutions of the compounds were added to final concentrations of 20 or 100 μg/ml at 25°C for 1 day. Then, nematodes were scored as alive or dead using an iRiSTM Digital Cell Imaging System (Logos Bio Systems, South Korea). At least three independent experiments were conducted using quadruplicate wells.
Kim Y.G., Lee J.H., Raorane C.J., Oh S.T., Park J.G, & Lee J. (2018). Herring Oil and Omega Fatty Acids Inhibit Staphylococcus aureus Biofilm Formation and Virulence. Frontiers in Microbiology, 9, 1241.
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