Infinity 2 lc system

Manufactured by Agilent Technologies

The Infinity II LC System is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It provides precise control and monitoring of mobile phase flow, temperature, and pressure to enable accurate and reliable separation and detection of chemical compounds.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) 1100 series system, by Agilent Technologies (1 mentions) Cortecs c18 column, by Waters Corporation (1 mentions) Symmetryprep c18 column, by Waters Corporation (1 mentions) Xevo g2 xs qtof, by Waters Corporation (1 mentions) Symmetry c18 column, by Waters Corporation (1 mentions) Nanodrop instrument, by Thermo Fisher Scientific (1 mentions) Avance 600 spectrometer, by Bruker (1 mentions) Avance 500 spectrometer, by Bruker (1 mentions) Eclipse plus c18, by Agilent Technologies (1 mentions)

4 protocols using infinity 2 lc system

UHPLC-DAD-RI Chromatographic Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chromatographic analyses were performed using a UHPLC Agilent Infinity II LC System equipped with a Diode Array Detector and a Refractive Index Detector.

Delgado-Osorio A., Navajas-Porras B., Pérez-Burillo S., Hinojosa-Nogueira D., Toledano-Marín Á., Pastoriza de la Cueva S., Paliy O, & Rufián-Henares J.Á. (2024). Cultivar and Harvest Time of Almonds Affect Their Antioxidant and Nutritional Profile through Gut Microbiota Modifications. Antioxidants, 13(1), 84.

+ Open protocol

+ Expand

Analytical Characterization of Chemical Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

UV spectra were recorded on a Nanodrop instrument (Thermo Scientific). NMR spectra were recorded on an Avance 500 spectrometer equipped with a TCI-500 triple-resonance cryoprobe (Bruker) and an Avance 600 spectrometer equipped with a TCI 1.7 mm triple-resonance microcryoprobe. NMR spectra were referenced to residual solvent signals (DMF-d₇, ¹H 2.92 and ¹³C 34.9; CD₃OD, ¹H 3.31 and ¹³C 49.1). HPLC-LRESIMS was carried out on an Infinity II LC system with a 6130 quadrupole MSD (Agilent) and a Symmetry C18 column (Waters, 5 μm, 4.6 × 150 mm), or an Acquity UPLC with a Xevo TQSu tandem quadrupole fitted with a UniSpray source (Waters) and a Cortecs C18 column (Waters, 1.7 μm, 2.1 × 100 mm). HRESIMS and MS/MS fragmentation was carried out on a Xevo G2-XS QTof (Waters). Semipreparative HPLC was performed on an 1100 series system (Agilent) using a SymmetryPrep C18 column (Waters, 7 μm, 7.8 × 300 mm). All solvents (HPLC grade) were obtained from Sigma-Aldrich. UV–Vis spectra were recorded on a Nanodrop 2000 (Thermo Scientific).

Davison J.R, & Bewley C.A. (2019). Antimicrobial Chrysophaentin Analogs Identified from Laboratory Cultures of the Marine Microalga Chrysophaeum taylorii. Journal of natural products, 82(1), 148-153.

+ Open protocol

+ Expand

Uric Acid Quantification via HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Uric acid in the culture supernatant was measured using HPLC. The supernatant was treated with 60% perchloric acid to remove proteins and was centrifuged at 10,000× g for 5 min at 4 °C. The samples were filtered through a 0.45 μm syringe filter before analysis (Advantec, 13HP045, Tokyo, Japan). HPLC separation was conducted using the Infinity II LC system (Agilent Technologies) equipped with an Intersil ODS-2 column (4.6 mm × 250 mm, 5 μm; GL Sciences) and maintained at 40 °C. The mobile phase was 98% of 74 mM phosphate buffer (pH 2.2), and 2% methanol and uric acid was detected by measuring the absorbance at 284 nm.

Yamasaki M., Kiue Y., Fujii K., Sushida M., Yamasaki Y., Sugamoto K., Suzuki Y., Koga Y., Kunitake H., Kai H., Ogawa K., Nishiyama K., Goto Y, & Nakayama T. (2021). Vaccinium virgatum Aiton Leaves Extract Suppressed Lipid Accumulation and Uric Acid Production in 3T3-L1 Adipocytes. Plants, 10(12), 2638.

+ Open protocol

+ Expand

HPLC Analysis of Gallic and Ellagic Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serial dilutions of the reference standards gallic acid and ellagic acid were prepared in methanol and 10% DMSO in methanol, respectively. The sample peaks were confirmed by comparing the reference standard peak in terms of the retention time (RT) and consistency using an ultraviolet (UV) detector. The analysis was performed by HPLC coupled with a UV detector (1,260 Infinity II LC System, Agilent), and the chromatographic separation assay was performed with an Eclipse Plus C18 analytical column (Agilent, 150 × 2.6 mm, 3.5-μm particle size) according to a previous study (25 (link)). The mobile phase consisted of 0.1% formic acid in distilled water (A) and acetonitrile (B), the flow rate was 0.12 ml/min, and the linear gradient program was the following: 0–30 min (95–70% A), 30–57 min (70–5% A), and 57–70 min (5–95% A). The injection volume was 2 μl, and the wavelength was set to 275 nm.

Kim J., Jo S., Choi Y., Kim T.W, & Park J.E. (2022). Chestnut inner shell extract inhibits viral entry of porcine epidemic diarrhea virus and other coronaviruses in vitro. Frontiers in Veterinary Science, 9, 930608.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!