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Sch23390

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SCH23390 is a selective dopamine D1 receptor antagonist. It binds to the D1 dopamine receptor with high affinity and selectivity, making it a useful tool for studying the function and distribution of this receptor subtype.

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Lab products found in correlation

Skf81297, by Bio-Techne (11 mentions) Sulpiride, by Bio-Techne (11 mentions) Skf38393, by Bio-Techne (8 mentions) Quinpirole, by Bio-Techne (7 mentions) Cgp55845, by Bio-Techne (7 mentions) L 741 626, by Bio-Techne (6 mentions) Bapta, by Thermo Fisher Scientific (6 mentions) Picrotoxin, by Bio-Techne (5 mentions) Prazosin, by Merck Group (5 mentions) Propranolol, by Merck Group (5 mentions) Scopolamine hydrobromide, by Bio-Techne (4 mentions) Forskolin, by Bio-Techne (4 mentions) Skf38393, by Abcam (4 mentions) K methylsulphate, by Thermo Fisher Scientific (3 mentions) Nomifensine, by Bio-Techne (3 mentions) Sch58261, by Bio-Techne (3 mentions) K methylsulfate, by Thermo Fisher Scientific (3 mentions) Sulpiride, by Merck Group (2 mentions) Dopamine, by Merck Group (2 mentions) Dihydrexidine, by Bio-Techne (2 mentions)

82 protocols using sch23390

1

Pharmacological Modulation of Neuronal Activity

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Where indicated, the following drugs were bath applied: α-adrenoceptor antagonist prazosin (prazosin hydrochloride, 419.86 g mol−1; Sigma-Aldrich) at a concentration of 30 μM, β-adrenoceptor antagonist propranolol [(S)-(−)-propranolol hydrochloride, 295.80 g mol−1; Sigma-Aldrich] at a concentration of 30 μM, D1/D5 receptor antagonist SCH 39166 (SCH 39166 hydrochloride, 394.73 g mol−1; Tocris) at a concentration of 100 nM (intracellular recordings, n = 2), and D1/D5 receptor antagonist SCH 23390 (SCH 23390 hydrochloride, 324.24 g mol−1; Tocris) at a concentration of 100 nM (intracellular recordings, n = 3) or 1 μM (extracellular recordings).
Takeuchi T., Duszkiewicz A.J., Sonneborn A., Spooner P.A., Yamasaki M., Watanabe M., Smith C.C., Fernández G., Deisseroth K., Greene R.W, & Morris R.G. (2016). Locus coeruleus and dopaminergic consolidation of everyday memory. Nature, 537(7620), 357-362.
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2

Pharmacological Modulation of Neuronal Activity

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Where indicated, the following drugs were bath applied: α-adrenoceptor antagonist prazosin (prazosin hydrochloride, 419.86 g mol−1; Sigma-Aldrich) at a concentration of 30 μM, β-adrenoceptor antagonist propranolol [(S)-(−)-propranolol hydrochloride, 295.80 g mol−1; Sigma-Aldrich] at a concentration of 30 μM, D1/D5 receptor antagonist SCH 39166 (SCH 39166 hydrochloride, 394.73 g mol−1; Tocris) at a concentration of 100 nM (intracellular recordings, n = 2), and D1/D5 receptor antagonist SCH 23390 (SCH 23390 hydrochloride, 324.24 g mol−1; Tocris) at a concentration of 100 nM (intracellular recordings, n = 3) or 1 μM (extracellular recordings).
Takeuchi T., Duszkiewicz A.J., Sonneborn A., Spooner P.A., Yamasaki M., Watanabe M., Smith C.C., Fernández G., Deisseroth K., Greene R.W, & Morris R.G. (2016). Locus coeruleus and dopaminergic consolidation of everyday memory. Nature, 537(7620), 357-362.
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3

Signaling Pathway Analysis in Neuronal Cells

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B-27, L-glutamic acid, deoxyribonuclease (DNase), fluorescent dye, anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), soybean trypsin inhibitor, fluo-8, and poly-l-lysine (PLL) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-p-p38 MAPK (p-p38), anti-p-JNK, anti-p-p44/42 MAPK (p-ERK1/2), anti-p38 MAPK, anti-JNK, anti-ERK1/2, and anti-rabbit/mouse IgG antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Donkey anti-rabbit/mouse secondary antibodies and Alexa Fluor 488 were obtained from Jackson ImmunoResearch Laboratories, Inc., (PA, USA). Fetal bovine serum, neurobasal medium, RPMI 1640 medium, and trypsin were purchased from Gibco (Gaithersburg, MD, USA). The Gαq inhibitor YM 254,890 was obtained from Wako Pure Chemical Industries (Osaka, Japan). L-741,626, SCH 23390, NMDA, and PP2, LY 23,959 were purchased from Tocris Bioscience (Ellisville, MO, UK). YM 254,896 and PP2 were dissolved in 2% DMSO, L-741,626 was dissolved in 25% DMSO, SCH 23390, NMDA and LY2359549 were dissolved in sterilized saline.
Dai W.L., Bao Y.N., Fan J.F., Ma B., Li S.S., Zhao W.L., Yu B.Y, & Liu J.H. (2020). Blockade of spinal dopamine D1/D2 receptor suppresses activation of NMDA receptor through Gαq and Src kinase to attenuate chronic bone cancer pain. Journal of Advanced Research, 28, 139-148.
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4

Electrophysiology Experiments: Pharmacological Agents

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All chemicals used for electrophysiology experiments were obtained from Sigma-Aldrich except D-L-AP5 and NBQX (from Ascent Scientific). The D1 Receptor agonist SKF 81297 hydrobromide (6-chloro-2,3,4,5-tetrahydro-1-phenyl-1H-3-benzazepine hydrobromide) and the D1R antagonist SCH 23390 hydrochloride ((R)-(+)-7chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride) were from Tocris. SKF81297 and SCH23390 were dissolved in water at a 10 mM concentration for stock solutions. Drugs were bath applied for 15 minutes before a “drug recording”.
Meunier C.N., Callebert J., Cancela J.M, & Fossier P. (2015). Effect of Dopaminergic D1 Receptors on Plasticity Is Dependent of Serotoninergic 5-HT1A Receptors in L5-Pyramidal Neurons of the Prefrontal Cortex. PLoS ONE, 10(3), e0120286.
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5

Dopaminergic Regulation of Th17 Cell Function

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To evaluate the function of Th17-cells, CD4+ T-cells were isolated and activated by anti-CD3/CD28-microbeads as previously described [17 (link)].
To assess the effect of dopamine on the function of Th17-cells, samples of CD4+ T-cells were cultured in the presence of dopamine (Sigma, USA) at a concentration of 10–5 M [9 (link)] for 15 min. whereafter anti-CD3/CD28-microbeads were added to the cultures.
To study the involvement of dopaminergic receptors in dopamine-mediated modulation of cytokine production, some samples of CD4+ T-cells were pre-incubated with antagonists of D1- or D2-like dopaminergic receptors (SCH23390 and sulpiride respectively) (both from Sigma, USA) at a concentration of 10–5 M [9 (link)] for 15 min, whereafter dopamine (at 10–5 M) was added to the cultures and stimulation was proceeded.
To study the direct effect of blockading or activation of the dopaminergic receptor, CD4+ T-cells were pre-incubated in the presence of D1- or D2-like receptors antagonists (SCH23390 and sulpiride respectively [at 10–5 M]) or D2-like receptor agonist (quinpirole [at 10–7 M] Tocris, Switzerland) and activated by anti-CD3/CD28-microbeads [18 (link)].
Melnikov M., Sviridova A., Rogovskii V., Kudrin V., Murugin V., Boyko A, & Pashenkov M. (2022). The Role of D2-like Dopaminergic Receptor in Dopamine-mediated Modulation of Th17-cells in Multiple Sclerosis. Current Neuropharmacology, 20(8), 1632-1639.
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6

Preparation of D1 Receptor Ligands

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SCH23390 (Tocris, Cat. #: 0925), a standard selective D1-like antagonist, was prepared as a 100 mM stock solution in saline and delivered at 1 mM final concentration in saline for cannulation experiments. The potent full efficacy D1R agonist Dihydrexidine (Tocris, Cat. #: 0884) was prepared as a 10 mM stock solution in deionized water.
Park A.J., Harris A.Z., Martyniuk K.M., Chang C.Y., Abbas A.I., Lowes D.C., Kellendonk C., Gogos J.A, & Gordon J.A. (2021). Reset of hippocampal-prefrontal circuitry facilitates learning. Nature, 591(7851), 615-619.
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7

Synthesis and Characterization of Stimulant Drugs

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α-PVP, α-PBP, and α-PPP were purchased from Cayman Chemical (Ann Arbor, MI, USA). SCH23390 was purchased from Tocris (Minneapolis, MN, USA). MDPV was synthesized in house at RTI using standard synthetic procedures. MDPV was formulated as a recrystallized HCl salt and was > 97% pure. The purity was assessed by several analytical techniques including carbon, hydrogen, nitrogen (CHN) combustion analysis and proton nuclear magnetic resonance spectroscopy. All drugs were dissolved in sterile saline (Butler Schein, Dublin, OH, USA). Doses are expressed as mg/kg of the salt, and were administered at a volume of 10 ml/kg in mice. Sterile saline was used as a comparison for all drugs for in vivo studies.
Marusich J.A., Antonazzo K.R., Wiley J.L., Blough B.E., Partilla J.S, & Baumann M.H. (2014). Pharmacology of novel synthetic stimulants structurally related to the “bath salts” constituent 3,4-methylenedioxypyrovalerone (MDPV). Neuropharmacology, 87, 206-213.
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8

Intracranial Drug Infusion Protocol

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JWH133, AM630, SCH23390 and L741,626 were purchased from Tocris Bioscience (Minneapolis, MN). Both JWH133 and AM630 were dissolved in DMSO for in vitro electrophysiology assays and in Tocrisolve-100 (vehicle) for intracranial microinjection. All other drugs were dissolved in saline.
Zhang H.Y., Shen H., Gao M., Ma Z., Hempel B., Bi G.H., Gardner E.L., Wu J, & Xi Z.X. (2021). Cannabinoid CB2 receptors are expressed in glutamate neurons in the red nucleus and functionally modulate motor behavior in mice. Neuropharmacology, 189, 108538.
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9

Immunochemical Analysis of Neuroinflammation

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The following primary antibodies were used throughout this study: rat anti-mouse CD11b (1:400, Abcam), rabbit anti-F-actin (1:1000, Abcam), rabbit anti-COX-2 (1:1000, Abcam), rabbit anti-IL-1β (1:200, Abcam), rabbit anti-GFAP (1:5000, Neuromics), rabbit anti-Iba-1 (1:1000, Wako), goat anti-Iba-1 (1:500, Wako), rabbit anti-AKT (1:1000, Santa Cruz), rabbit anti-p-AKT (Ser473, Thr308) (1:1000, Cell Signaling), rabbit anti-ERK (1:1000, Santa Cruz), rabbit anti-p-ERK (Thr42/44) (1:1000, Cell Signaling), rabbit anti-STAT3 (1:1000, Cell Signaling), rabbit anti-p-STAT3 (Ser727, Abcam), mouse anti-PCNA (1:1000, Santa Cruz), rabbit anti-D2R (1:1000, Abcam), and rabbit anti-D1R (1:1000, Millipore) antibodies. We used the following small molecules: D1R antagonists (LE300, 10 μM, Sigma-Aldrich; SCH23390, 30 μM, Tocris), D1R agonist (A77636 hydrochloride, 10 nM, Tocris), D2R antagonist (eticlopride hydrochloride, 100 nM, Sigma-Aldrich), a STAT3 inhibitor (S3I-201, 50 μM, Sigma-Aldrich), and an ERK inhibitor (PD98059, 10 μM, Millipore).
Lee J.Y., Nam J.H., Nam Y., Nam H.Y., Yoon G., Ko E., Kim S.B., Bautista M.R., Capule C.C., Koyanagi T., Leriche G., Choi H.G., Yang J., Kim J, & Hoe H.S. (2018). The small molecule CA140 inhibits the neuroinflammatory response in wild-type mice and a mouse model of AD. Journal of Neuroinflammation, 15, 286.
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10

Preparation of SCH 23390 and Cocaine

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SCH 23390 (Tocris) was dissolved in saline (0.9% NaCl) to a concentration of 2 µg/0.3 µl. Cocaine hydrochloride was dissolved in saline to a concentration of 2 mg/ml.
Kim E.S, & Lattal K.M. (2019). Context-Dependent and Context-Independent Effects of D1 Receptor Antagonism in the Basolateral and Central Amygdala during Cocaine Self-Administration. eNeuro, 6(4), ENEURO.0203-19.2019.
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