High-performance liquid chromatography (HPLC) system was consisted of a chromatographic pump (P680, Dionex, Germany), an automated sample injector (ASI-100, Dionex, Germany) and a thermostatted column compartment (TCC-100, Dionex, Germany) equipped with evaporative light scattering detector (ELSD) (PL-ELS2100, Polymer Laboratories, UK). Chromatographic separation was achieved on a Shiseido CapCell PAK C18 column (5 μm, 4.6 mm i.d. × 150 mm); the column temperature was set at 30°C. The mobile phase was consisted of (A) 0.1% formic acid and (B) acetonitrile at a flow rate of 1.0 mL/min, with gradient elution as follows: 0 min, 20% B; 18 min, 20% B; 25 min, 60% B; 45 min, 60% B; 45.1 min, 85% B; 60 min, 85% B; v/v). The temperature of evaporator for ELSD was set at 50tDowith a nitrogen gas flow rate of 1.5 L/min. The aliquot of JSE1-4 (10 μl/ml of 10 mg/ml) was injected into HPLC-ELSD system, respectively, to evaluate amounts of the active components.
Tcc 100
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States
The TCC-100 is a laboratory instrument designed for thermal conductivity measurements. It provides accurate and reliable data on the thermal conductivity of various materials. The core function of the TCC-100 is to measure the thermal transport properties of samples under controlled temperature conditions.
Automatically generated - may contain errors
Lab products found in correlation
Asi 100, by Thermo Fisher Scientific (6 mentions)
Ultimate 3000, by Thermo Fisher Scientific (3 mentions)
Capcell pak c18 column, by Shiseido (2 mentions)
Wps 3000rs, by Thermo Fisher Scientific (2 mentions)
Pl els2100, by Polymer Laboratories (1 mentions)
Sb c18 analytical column, by Agilent Technologies (1 mentions)
Pda 100, by Thermo Fisher Scientific (1 mentions)
Agilent 1260 infinity hplc system, by Agilent Technologies (1 mentions)
Chromeleon chromatography data system, by Thermo Fisher Scientific (1 mentions)
Uvd340u detector, by Thermo Fisher Scientific (1 mentions)
Dad 3000rs, by Thermo Fisher Scientific (1 mentions)
Securityguard, by Phenomenex (1 mentions)
Dionex p680 pump, by Thermo Fisher Scientific (1 mentions)
Uvd100, by Thermo Fisher Scientific (1 mentions)
Dionex, by Thermo Fisher Scientific (1 mentions)
12 protocols using tcc 100
1
HPLC-ELSD Analysis of Active Components
2
HPLC Analysis of Butorphanol and Ketamine
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The Dionex HPLC system consisted of a UltiMate 3000 quaternary gradient pump, an ASI-100 auto sampler, a TCC-100 thermostat column oven, an ultraviolet detector (DAD), and a data system (Chromeleon® version 6.80). HPLC separation was performed on a Zorbax SB-C18 analytical column (150×4.6 mm, 5.0 μm). The mobile phase consisted of 0.05 mol/L KH2PO4 and acetonitrile in the proportion of 70: 30 (v/v), with a flow rate of 1.0 ml/min. The wavelength was set at 280 nm for butorphanol and 268 nm for ketamine. The column temperature was kept ambient and injection volume was 20 μL.
3
Isoflavone Composition and Quantitation of RCE
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The post-fermentation isoflavone composition and quantitation analysis of the RCE was performed by DB Lab A/S (Odense, Denmark). Quantitation was assessed using high performance liquid chromatography with ultraviolet and mass spectrometry detection. Briefly, a Summit® LC/MS (Sunnyvale, CA, USA) system consisting of a quaternary pump (P680 LPG), autosampler (ASI 100T), column oven (TCC-100) UV detector (PDA-100) and MS detector (Surveyor MSD Plus), all from Dionex, were used to perform the analysis. Four standards of the primary isoflavones (genistein, daidzein, formononetin and Biochanin A) and two glycoside derivatives (Ononin and Sissotrin) were obtained from Sigma Aldrich Denmark A/S (Brødby, Denmark). The RC extract was diluted 10 times (50ml to 5ml) by methanol prior to analysis.
Analysis (S1 Table ) revealed that the majority of the isoflavones (≥90%) were converted to aglycones, although total conversion was not achieved (verified by the presence of ononin and sissotrin glucosides). In accordance with the DBlab analysis the participants who received active treatment were given a dose of 37.1 mg/d (of which 33.78 mg/d were aglycones). Due to lack of available standards other isoflavone glycosides were not included within the analysis.
Analysis (
4
Quantitative Sugar Profiling in Fruits
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Fruit sugar extraction was conducted according to our previous study (Vimolmangkang et al., 2016 (link)). Measurement of sugar type and content was performed using high-performance liquid chromatography (HPLC) following the previously reported protocol (Ma et al., 2015 (link)). Briefly, the sugar contents of fruit samples were measured using an Agilent 1260 Infinity HPLC system (Milford, MA, USA) equipped with a refractive index detector (Shodex RI-101; Shodex Munich, Germany). A Transgenomic COREGET-87C column (7.8 mm × 300 mm, 10 μm) with a guard column (Transgenomic CARB Sep Coregel 87C) was used to perform separation and column temperature was maintained at 85°C by a Dionex TCC-100 thermostated column compartment. Flow rate at the mobile phases were maintained at the rate of 0.6 mL/min with degassed, distilled, and deionized water. Sugar concentrations were expressed on a fresh weight (FW) basis. Total sugar content was specified by the amount of four sugar components, sucrose, fructose, glucose, and sorbitol.
5
HPLC Quantification of Salvianolic Acid B in Herbal Extracts
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The quantitation of major compound, salvianolic acid B, in SM extract was performed by HPLC analysis. The HPLC-DAD system consisting of a chromatographic pump (P680, Dionex, Idstein, Germany), an automated sample injector (ASI-100, Dionex), and a thermostatted column compartment (TCC-100, Dionex) equipped with an UVD 340U detector (Dionex). The chromatogram of HPLC system was recorded by using Dionex’s Chromeleon™ Chromatography Data System. The column used in this work was a Capcell Pak C-18 column (150 mm × 4.6 mm, I.D., 5 μm, Shiseido Co., Ltd., Japan.). The column temperature was set to 30 °C. The mobile phase consisted of (A) 0.1 % formic acid in water and (B) 100 % acetonitrile at a flow rate of 1.0 mL/min. The gradient condition was as follows: 0–5 min, 10 %–30 % B; 5–25 min, 30 %–40 % B; 25–30 min, 40 %–100 % B.
6
Quantitative Analysis via HPLC
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High-performance liquid chromatography (HPLC) was performed to evaluate the sample concentrations. The HPLC instrument (Ultimate 3000, Dionex, Germering, Germany) was equipped with a quaternary liquid gradient system, WPS-3000RS auto-injector, TCC-100 column oven, and DAD-3000RS UV spectrophotometer. Chromoleon chromatography software (version 6.80) was used for the chromatographic data acquisition and analysis. The pH of each solution was measured with a PHS-3C pH meter (Shanghai Leici Instrument Co., China).
7
HPLC Analysis of Phytochemical Extract
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HPLC analysis was performed using a Dionex (Sunnyvale, CA, USA) system comprising automated sample injector (ASI-100), pump (P680), thermostat column compartment (TCC-100), ultra-violet detector (UVD-170U) running Chromeleon 6.80 software. The effluent was monitored by UV detection at 254 nm. Separation column: YMC C18 column (4.6 mm I.D.× 250 mm, 5 μm); column temperature: 32°C; the mobile phase: methanol (A), and 0.4% phosphoric acid (B) that followed: A-B in 50 min. Flow rate: 1.0 mL/min. To prepare analytical samples, PRE powder was accurately weighed and dissolved in 50% ethanol at a concentration of 50 mg/mL. Prior to analysis, sample was filtered through a 0.45 μm filter.
8
Urea and Palmitate Turnover Assessment
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Urea turnover was assessed as previously described. [22] (link) A primed (309.6 mg) continuous 13 Ccarbamide infusion of 42 mg hour -1 was given during basal period (0-180 min), and 13 Ccarbamide enrichment was measured using high-performance liquid chromatography (HPLC; RF2000 fluorescence detector, P680 pump, ASI-100, TCC100; Dionex, Amsterdam, The Netherlands). [25] (link) Palmitate Systemic palmitate turnover was assessed as previously described. [21] (link) Albumin-bound 9,10-3 H-palmitate (GE Healthcare) was infused continuously (0.3 µCi min -1 ) during basal (120-180 min), low-dose insulin infusion (240-300 min), and high-dose insulin infusion period (360-420 min). Blood samples for measurement of specific activity and palmitate concentration were drawn before infusion (0, 240, 360 mins) and in triplicates at the end of infusions (160-180; 280-300; 400-420 mins).
9
HPLC Analysis of Sample Components
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Each sample was analyzed by high-performance liquid chromatography (HPLC) to quantify the components. The HPLC system (Ultimate 3000, Dionex, Germering, Germany) consisted of a quaternary liquid gradient system, WPS-3000RS autoinjector, TCC-100 column oven, and DAD-3000RS UV spectrophotometer. Chromatographic data were acquired using Chromeleon software version 6.80. The pH values for the samples at each designated time interval were measured with a pH meter (Model pHS-3C, Leici Instrument Co., Shanghai, China).
10
HPLC Analysis of Aflatoxins
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The aflatoxins were extracted according to the method reported by Cavallarin et al. (2011) . Sample extracts were stored at -20°C until HPLC analysis. The HPLC apparatus consisted of a Dionex P680 pump (Dionex, Sunnyvale, CA) equipped with a Rheodyne Model 7725i injection valve (Rheodyne, Rohnert Park, CA), a Dionex RF-2000 fluorimetric detector (excitation wavelength = 365 nm, emission wavelength = 435 nm for AFB 1 , AFB 2 , AFG 1 , and AFG 2 ), a Dionex TCC-100 thermostatted column compartment, and a Chromeleon6 data handling system (Dionex). The analytical column was a ProdigyODS 2 (150 × 4.6 mm, 5-μm particles; Phenomenex, Torrance, CA), which was preceded by a SecurityGuard (Phenomenex) guard column.
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