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2 protocols using c myc 9e10

1

Comprehensive Antibody and Reagent Source

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The following antibodies K19 (A53-B/A2), GSK3β (E-11), pGSK3β (F-2), PARP-1 (F-2), β-actin (C4), and GAPDH (0411) were from Santa Cruz Biotechnology (Santa Cruz, CA); cyclin D3 (DCS22) and GSK3β (D5C5Z) were from Cell Signaling Technology (Danvers, MA); GFP (3H9) and RFP (5F8) were from Chromotek (Islandia, NY); cyclin D3 (26755-1-AP) was from Proteintech (Rosemont, IL); and anti-tubulin (12G1), anti-GFP (1D2), and c-Myc (9E10) were from Developmental Studies Hybridoma Bank (Iowa City, IA). Forskolin was from ApexBio (#B1421) (Houston, TX), LiCl (# EM-LX0331) and DMSO were from VWR Life Sciences (Carlsbad, CA), LY294002 was from AmBeed (#A133122) (Arlington Hts, IL), SB 203580 (#S-3400) and SB 202190 (S-1700) were from LC Laboratories (Woburn, MA), okadaic acid (459616) was from EMD Millipore (Billerica, MA), cycloheximide (#66-81-9) was from Sigma-Aldrich (St Louis, MO), and MG132 (S2619) was from Selleck Chemicals.
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2

Antibodies for Protein Localization

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Polyclonal antisera against the following proteins were used: Rab5 (Cell Signaling Technology); TYRP1 (H-90), c-Myc (A-14 for immunoblotting) and EEA1 (goat) (Santa Cruz Biotechnology). Other antisera to VAMP7 (a gift from Andrew Peden, University of Sheffield, Sheffield, UK); STX13 (Prekeris et al., 1998 (link)); and TYR (Theos et al., 2005 (link)) are as described previously. Monoclonal antisera against the following proteins were used: PMEL (HMB45, Abcam); TYRP1 (TA99, ATCC); adaptin γ (BD Biosciences, AP-1); adaptin δ (SA4, AP-3 used for immunofluorescence microscopy), LAMP-1 (1D4B), LAMP-2 (GL2A7) and c-Myc (9E10) (Developmental Studies Hybridoma Bank); adaptin σ3 (Santa Cruz Biotechnology); γ-tubulin (Sigma-Aldrich). Secondary antibodies were either from Molecular Probes (Life Technologies) or Jackson Immunoresearch.
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