Cell culture dishes, plates, centrifuge tubes, and other plastic ware were purchased from BD Biosciences (Lincoln Park, NJ); Dulbecco modified Eagle medium (DMEM), Ham F-12, amphotericin B, and gentamicin were from Invitrogen (Grand Island, NY). Fetal bovine serum (FBS) was from Hyclone (Logan, UT). RNeasy Plus Mini RNA extraction kit from Qiagen (Valencia, CA); Ready-To-Go-Primer First-Strand Beads were from GE Healthcare (Piscataway, NJ); TaqMan gene expression assays and real-time PCR master mix were from Applied Biosystems (Foster City, CA). DCFDA—Cellular Reactive Oxygen Species Detection Assay Kit, rabbit polyclonal antibody against human malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), 4-hydroxy-2-nonenal (HNE), aconitase-2, glutathione peroxidase-1 (GPX1), and mouse monoclonal antibody against superoxide dismutase-1 (SOD1) were purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibody against human heme oxygenase-1 (HMOX1) and cyclooxygenase-2 (COX2) were from Santa Cruz Biotechnology (Santa Cruz, CA). β-actin antibody was from BioLegend (San Diego, CA). Fluorescein Alexa-Flour 488-conjugated secondary antibodies (donkey anti-rabbit, or goat anti-mouse IgG) were from Molecular Probes (Eugene, OR).
Cyclooxygenase 2 cox 2
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Cyclooxygenase-2 (COX-2) is an important enzyme that plays a crucial role in the production of prostaglandins, which are involved in various physiological and pathological processes. It is an essential tool for researchers studying inflammation, pain, and related conditions.
Automatically generated - may contain errors
Lab products found in correlation
Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions)
Centrifuge tube, by BD (1 mentions)
Gentamicin, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
Real time pcr master mix, by Thermo Fisher Scientific (1 mentions)
Hmox1, by Santa Cruz Biotechnology (1 mentions)
Ready to go primer first strand beads, by GE Healthcare (1 mentions)
β actin antibody, by BioLegend (1 mentions)
Cell culture dishes, by BD (1 mentions)
Dcfda cellular reactive oxygen species detection assay kit, by Abcam (1 mentions)
Rneasy plus mini rna extraction kit, by Qiagen (1 mentions)
Glutathione peroxidase 1 gpx1, by Abcam (1 mentions)
Succinic acid, by Merck Group (1 mentions)
Taurine, by Merck Group (1 mentions)
Dopamine, by Merck Group (1 mentions)
Indole, by Merck Group (1 mentions)
Guanosine, by Merck Group (1 mentions)
Kynurenic acid, by Merck Group (1 mentions)
P cresol sulfate, by Merck Group (1 mentions)
Uracil, by Merck Group (1 mentions)
8 protocols using cyclooxygenase 2 cox 2
1
Oxidative Stress Biomarkers Analysis
2
Analytical Protocol for Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Creatinine, hippuric acid, uric acid, hypoxanthine, xanthine, myoinositol, cis-aconitic acid, indoxyl sulfate and aldosterone were obtained from the National Institutes for Food and Drug Control (Beijing, China). Amino acids including l -methionine, l -lysine, l -phenylalanine, l -homoserine, homocysteine, l -tyrosine, l -glutamine, citrulline, l -arginine, l -cysteine, l -glutamic acid, l -alanine and L -aspartic acid were purchased from Amresco Company. Kynurenine, kynurenic acid, dopamine, 1-methyladenosine, l -xanthosine, xanthurenic acid, indole, p-cresol sulfate, uracil, p-cresol, guanosine, succinic acid, deoxyuridine, guanine, taurine and thymine were purchased from Sigma Company or Aladdin Company. Antibodies against nuclear factor kappa B p65, Nrf2, cyclooxygenase-2 (COX-2), 12-lipoxygenase (12-LP), etc. were purchased from Santa Cruz Biotechnology or Abcam Company.
3
Histological and Immunohistochemical Analysis of Mouse Ankle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following euthanasia, mouse ankles were removed, fixed in 4% paraformaldehyde (Sigma, USA) and decalcified with Calci-Clear Rapid Solution (National Diagnostics, USA). The ankles were embedded in paraffin, sectioned and stained with hematoxylin and eosin (H&E) (Sigma, USA) for histological examination.
For immunohistochemistry, ankle specimens were blocked with 3% methanolic hydrogen peroxide and then digested. Formalin-fixed, paraffin-embedded tissues were exposed to pepsin (Invitrogen, USA; 1:200) by dropping it on the slide at 37°C for 10 min to unmask antigens. After blocking with 3% bovine serum albumin, the sections were incubated with primary rat antibodies against cartilage oligomeric matrix protein (COMP; 1:50, Antibodies Online, Germany), cyclooxygenase-2 (COX2; 1:100, Santa Cruz Biotechnology, USA), IL-6 (1:50, Antibodies Online, Germany), matrix metalloproteinase-3 (MMP3; 1:100, Santa Cruz Biotechnology, USA), MMP14 (1:100, Abcam, England) and TNF-α (1:100, Santa Cruz Biotechnology, USA). Staining was visualized using immunoperoxidase (BD Biosciences, USA).
For immunohistochemistry, ankle specimens were blocked with 3% methanolic hydrogen peroxide and then digested. Formalin-fixed, paraffin-embedded tissues were exposed to pepsin (Invitrogen, USA; 1:200) by dropping it on the slide at 37°C for 10 min to unmask antigens. After blocking with 3% bovine serum albumin, the sections were incubated with primary rat antibodies against cartilage oligomeric matrix protein (COMP; 1:50, Antibodies Online, Germany), cyclooxygenase-2 (COX2; 1:100, Santa Cruz Biotechnology, USA), IL-6 (1:50, Antibodies Online, Germany), matrix metalloproteinase-3 (MMP3; 1:100, Santa Cruz Biotechnology, USA), MMP14 (1:100, Abcam, England) and TNF-α (1:100, Santa Cruz Biotechnology, USA). Staining was visualized using immunoperoxidase (BD Biosciences, USA).
4
Immunohistochemical Analysis of Brain Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded brain sections were cut at 5 μm and affixed to slides to ensure adhesion. After deparaffinization and hydration, the slides were washed in TBS (10 mM/l Tris HCl, 0.85% NaCl, pH 7.5) containing 0.1% bovine serum albumin (BSA). Endogenous peroxidase activity was quenched by incubating the slides in methanol and 0.6% H2O2 in methanol. The primary antibodies, inducible-nitric oxide synthase (iNOS; 1:200) and cyclo-oxygenase-2 (COX-2; 1:200) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were left overnight at 4°C. The slides were washed in TBS and then horseradish peroxidase (HRP)-conjugated secondary antibody (1:500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was added and the slides were further incubated at room temperature for 45 minutes. The slides were washed in TBS and incubated with diaminobenzidine tetrahydrochloride as the substrate, counterstained with hematoxylin, and observed under a light microscope.
5
MHY3200 Antioxidant and Anti-inflammatory Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MHY3200 was designed and synthesized by Prof. H. R. Moon at the College of Pharmacy, Pusan National University, Busan, Korea [7 (link)], and dissolved in dimethyl sulfoxide (DMSO). Other chemical reagents were obtained from Sigma–Aldrich (St. Louis, MO, USA). 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) was purchased from Molecular Probes Inc. (Eugene, OR, USA). ECL Western Blotting Detection Reagents, Pierce bicinchoninic acid (BCA) protein assay kit, polyvinylidene difluoride (PVDF) membrane, and Dokdo-MARK™ protein size marker were purchased from GE Healthcare Biosciences (Buckinghamshire, UK), Thermo Fisher Scientific (Rockford, IL, USA), the Millipore Corporation (Bedford, MA, USA), and ElpisBiotech (Daejeon, Korea), respectively. Anti-NOX4, anti-p-Akt (Ser 473), manganese superoxide dismutase (MnSOD), p-p65 (Ser 276), p65, β-actin, transcription factor IIB (TFIIB), and cyclooxygenase-2 (COX-2) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against p-FoxO1 (Ser 256) were purchased from Cell Signaling Technology (Danvers, MA, USA) while anti-rabbit IgG-horseradish peroxidase-conjugated antibodies, anti-mouse IgG-horseradish peroxidase-conjugated antibodies, and anti-goat IgG-horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
6
Surfactin and DSS-Induced Colitis Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercial surfactin (CS) from Bacillus subtilis was obtained from Sigma-Aldrich (St. Louis, MO, USA). DSS (M.W. 36,000–50,000 Da) was obtained from MP Biomedicals (Irvine, CA, USA). ELISA kits for IL-1β, IL-6, tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MPC-1) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). NLRP3 antibody was obtained from Adipogen Life Science (SanDiego, CA, USA). IL-1β antibody was obtained from R&D Systems (Minneapolis, MN, USA). Antibodies against Actin, ASC, cyclooxygenase-2 (COX-2) and 5-ASA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A myeloperoxidase (MPO) activity assay kit was obtained from Abcam (Cambridge, UK).
7
Quantifying Protein Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot analysis was performed as described previously.15 (link),20 (link) The membrane containing transferred protein was incubated with the following primary antibodies overnight at 4°C: Rabbit polyclonal antibodies against signal transducer and activator of transcription 3 (STAT3; cat. no. sc-482; dilution, 1:400; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), phospho (p)-STAT3 (cat. no. sc-8059; dilution, 1:500; Santa Cruz Biotechnology, Inc.), p65 (cat. no. sc-7151; dilution, 1:400; Santa Cruz Biotechnology, Inc.) and cyclooxygenase-2 (COX-2; cat. no. sc-23,983; dilution, 1:400; Santa Cruz Biotechnology, Inc.). Following washing with 0.1% Tween-20 in PBS, the membranes were then incubated with the following horseradish peroxidase-conjugated secondary antibodies for 1 h at 37°C: Goat anti-rabbit immunoglobulin G (IgG; cat. no. sc-2004; dilution, 1:2,000; Santa Cruz Biotechnology, Inc.). Proteins were detected using an enhanced chemiluminescence kit (GE Healthcare Life Sciences, Chalfont, UK). Anti-GAPDH monoclonal antibody (cat. no. ab9485; dilution, 1:2,500; Abcam, Cambridge, MA, USA) was used to ensure equal loading of protein.
8
Oleuropein Modulation of Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oleuropein (from coumarins, ≥ 98%) was purchased from Sigma-Aldrich (St. Louis, MO, USA) (Fig. 1A), prepared as a 100-mM stock solution in dimethyl sulfoxide (DMSO), and stored at -20°C. The Cell Counting Kit-8 (CCK-8) and 4',6-diamidino-2-phenylindole (DAPI) solution were purchased from Sigma-Aldrich. Culture medium was developed to a nal concentration ≤ 0.1% in DMSO, as previously described [19] . We purchased IL-1β and enzyme-linked immunosorbent assay (ELISA) kits from R&D Systems (Minneapolis, MN, USA). Antibodies raised against β-actin, cyclooxygenase-2 (COX-2), and heme oxygenase (HO)-1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!