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Fitc and pe conjugated anti mouse cd8a clone 53

Manufactured by BD

FITC and PE-conjugated anti-mouse CD8a (clone 53-6-7) are fluorescent-labeled antibodies that bind to the CD8a surface marker on mouse T cells. The FITC and PE conjugates allow for the detection and identification of CD8+ T cells using flow cytometry or other fluorescence-based techniques.

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2 protocols using fitc and pe conjugated anti mouse cd8a clone 53

1

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
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2

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
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