Ampfistr identifier kit
The AmpFISTR Identifier kit is a genetic analysis tool designed for forensic and human identification applications. It enables the amplification and detection of specific DNA markers to generate a genetic profile for identification purposes. The kit provides the necessary reagents and protocols to perform the analysis.
Lab products found in correlation
23 protocols using ampfistr identifier kit
Sensitizing Cervical Cancer Cells
Isolating and Authenticating GBM Neurospheres
Patient-Derived Glioma Sphere Culture
Culturing Human Neuroblastoma Cell Lines
Co-culture of AML Cells with MSCs
Co-culture experiments were performed by incubating AML cells with a feeder layer of human mesenchymal stem cells (MSC) derived from normal bone marrow samples in accordance with institutional guidelines. MSC cultures were plated for 24 h at 37 °C with 5% CO2 in a culture medium consisting of 20% FBS (Fisher Scientific, Hanover Park, IL, USA), α-MEM (Corning, Manassas, VA, USA) and 4% Human Platelet Lysate (EMD Millipore Corp, Billerica, MA, USA). Thereafter, the culture medium was removed and AML cells (OCI/AML3) were seeded on top of the MSC layer in a ratio of 4:1 (OCI/AML3:MSC) in RPMI-1640 and 10% FBS. Co-cultured cells were kept for 24 h at 37 °C with 5% CO2 before treatment (see below).
Culturing Human Neuroblastoma Cell Lines
Neuroblastoma Cell Line Characterization
Glioma Initiating Cells Isolation and Characterization
Multiple Myeloma Cell Lines Characterization
Characterizing FLT3 Mutations in Leukemia Cell Lines
AML patients’ samples were obtained after written informed consent following institutional guidelines of the University of Texas MD Anderson Cancer Center and in accordance with the principles of the Declaration of Helsinki. Mononuclear cells were purified from primary samples by Ficoll-Hypaque (Sigma-Aldrich) density-gradient centrifugation and were cultured in RPMI 1640 culture medium supplemented with 10% fetal bovine serum before treatment.
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