The reagents used were: Buspirone (Ansitec® - LIBBS Pharmaceutical LTDA, Embu-Guaçu, SP, Brazil); Diazepam (Cristália, Itapira, SP, Brazil); dimethylsulfoxide (10% DMSO, Sigma-Aldrich St. Louis, MO, USA); Flumazenil (União Química, Embu-Guaçu, SP, Brazil); Hibalactone (HB - isolated from H. umbellata); NAN-190 (1-(2-methoxyphenyl)-4-[4-(2-(2-phthalimido)butyl]piperazine hydrobromide - Sigma-Aldrich, St. Louis, MO, EUA); Polysorbate 80 (Tween 80® - Sigma-Aldrich, St. Louis, MO, USA); Saline solution (0.9%, NaCl - Belga). The HB was solubilized in 10% DMSO, and then distilled water was added to the desired concentration. The buspirone and Diazepam were prepared in distilled water. The NAN-190 was solubilized in 2% Tween 80® and dissolved in 0.9% saline. A 10% DMSO solution in distilled water was used as the vehicle. All compounds were administered at a volume of 10 mL/kg. Doses of the drugs were chosen according to the literature data.10 (link),13 (link),14 (link)
Nan 190
Manufactured by Merck Group
Sourced in Brazil
NAN-190 is a chemical compound used in scientific research and laboratory settings. It functions as a selective 5-HT1A receptor antagonist. The core purpose of NAN-190 is to facilitate the study of serotonin receptor interactions and related biological processes. Further details on intended use or applications are not provided to maintain an unbiased and factual presentation.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Polysorbate 80, by Merck Group (1 mentions)
Diazepam, by Cristália (1 mentions)
Bromodeoxyuridine (brdu), by Merck Group (1 mentions)
8 oh dpat, by Merck Group (1 mentions)
Caffeine, by Merck Group (1 mentions)
Picrotoxin, by Merck Group (1 mentions)
Dl ap5, by Merck Group (1 mentions)
Cocaine hcl, by Merck Group (1 mentions)
Fluoxetine, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Fluoxetine flu, by Merck Group (1 mentions)
Sulpiride, by Merck Group (1 mentions)
Ritanserin, by Merck Group (1 mentions)
5 protocols using nan 190
1
Antidepressant-like Effects of Hibalactone
2
Neurotransmitter Receptor Ligand Assay
3
Pharmacology of Neuroactive Compounds
4
Fluoxetine and Serotonin Receptor Ligands
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluoxetine, (±)−8-Hydroxy-2-(dipropylamino)tetralin hydrobromide (8-OH-DPAT), and 1-(2-Methoxyphenyl)-4-[4-(2-phthalimido)butyl] piperazine hydrobromide (NAN-190) were purchased from Sigma-Aldrich (St Louis, MO, USA) and were dissolved in saline. Fluoxetine (10 mg/kg/d, Sigma-Aldrich, St Louis, MO, USA) was intraperitoneally injected between 10:00 AM-12:00 PM for 14 days. 8-OH-DPAT (0.1 mg/kg/d) and NAN-190 (0.3 mg/kg/d) was intraperitoneally injected. NAN-190 was injected 30 minutes before Fluoxetine injection.
5
Standardized Evaluation of H. gordonii Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, a commercial dry purified extract of H. gordonii was
used, legally imported from Naturalmedis (UK). This product is manufactured in
accordance with the requirements of the Medicines and Healthcare Products Regulatory
Agency in the United Kingdom. The active ingredient content (P57) is guaranteed
through regular analyses performed by the company's Laboratory for Quality Control.
Also, the material was shown to be free of microbiological contamination and was at a
dilution of 20:1. The purchase of the substance was made for research purposes. For
the use of the drug in our experiments, H. gordonii was emulsified
in 0.3% Tween 20 (Sigma, USA) and diluted in distilled water. The extract was
administered orally to mice, at doses of 25 and 50 mg/kg in a single dose or for 15
consecutive days.
The additional drugs used is this study were: 10 mg/kg imipramine (IMI; Geisy,
Brazil), 1 mg/kg prazosin (PZS; Sigma), 1 mg/kg yohimbine (YOHI; Sigma), 0.05 mg/kg
SCH23390 (Sigma), 50 mg/kg sulpiride (Sigma), 100 mg/kg
p-chlorophenylalanine methyl ester (PCPA; Sigma), 0.5 mg/kg NAN-190
{1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]piperazine, Sigma}, 1 mg/kg
ondansetron (Sigma), 1 mg/kg ritanserin (Sigma), and 35 mg/kg fluoxetine (FLU;
Sigma). All drugs were diluted in distilled water and administered by the
intraperitoneal (ip) route.
used, legally imported from Naturalmedis (UK). This product is manufactured in
accordance with the requirements of the Medicines and Healthcare Products Regulatory
Agency in the United Kingdom. The active ingredient content (P57) is guaranteed
through regular analyses performed by the company's Laboratory for Quality Control.
Also, the material was shown to be free of microbiological contamination and was at a
dilution of 20:1. The purchase of the substance was made for research purposes. For
the use of the drug in our experiments, H. gordonii was emulsified
in 0.3% Tween 20 (Sigma, USA) and diluted in distilled water. The extract was
administered orally to mice, at doses of 25 and 50 mg/kg in a single dose or for 15
consecutive days.
The additional drugs used is this study were: 10 mg/kg imipramine (IMI; Geisy,
Brazil), 1 mg/kg prazosin (PZS; Sigma), 1 mg/kg yohimbine (YOHI; Sigma), 0.05 mg/kg
SCH23390 (Sigma), 50 mg/kg sulpiride (Sigma), 100 mg/kg
p-chlorophenylalanine methyl ester (PCPA; Sigma), 0.5 mg/kg NAN-190
{1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]piperazine, Sigma}, 1 mg/kg
ondansetron (Sigma), 1 mg/kg ritanserin (Sigma), and 35 mg/kg fluoxetine (FLU;
Sigma). All drugs were diluted in distilled water and administered by the
intraperitoneal (ip) route.
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