Gl261

Manufactured by Thermo Fisher Scientific

Sourced in United States

The GL261 is a compact and versatile laboratory instrument designed for general-purpose applications. It features a touchscreen interface and offers a range of functionalities to support various laboratory tasks. The details of its core function and intended use are not available in this factual and unbiased description.

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Lab products found in correlation

15 protocols using gl261

Culturing Mouse Cancer Cell Lines

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Neuro2A
(mouse neuroblastoma)
and GL-261 (mouse glioblastoma) lines were obtained from the American
Type Culture Collection (ATCC). Neuro2A and GL-261 cells were maintained
in Dulbecco’s modified Eagle’s medium (DMEM) supplemented
with 10% FBS (Gibco), penicillin/streptomycin (1×), Non-Essential
Amino Acids Solution (1×), and sodium pyruvate (1×) at 37
°C in a humidified atmosphere supplemented with 5% CO₂.

Reddy R.G., Surineni G., Bhattacharya D., Marvadi S.K., Sagar A., Kalle A.M., Kumar A., Kantevari S, & Chakravarty S. (2019). Crafting Carbazole-Based Vorinostat and Tubastatin-A-like Histone Deacetylase (HDAC) Inhibitors with Potent in Vitro and in Vivo Neuroactive Functions. ACS Omega, 4(17), 17279-17294.

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Glioma Cell Lines and Macrophage Cultivation

Cited 1 time

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Glioma cell lines U87 and human mononuclear macrophage line (THP-1) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Human microglia line HMC3 and murine glioma cell line GL261 were obtained from American Type Culture Collection (Manassas, VA, USA). The extraction of the patient-derived primary glioma cells (PGC28) were extracted as previously described.^{21 (link)} U87 and GL261 were maintained in Dulbecco’s modified Eagle’s medium (D MEM, 10566024, Gibco) supplemented with 10% fetal bovine serum (FBS, 16140071, Gibco) and 1% penicillin/streptomycin (10378016, Gibco). PGC28 and THP-1 were maintained in RPMI-1640 medium (61870036, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. THP-1 monocytes were primed with 5 nM PMA (P1585, Sigma) for 48 hours to become THP1-derived macrophages. HMC3 were maintained in Minimum Essential Medium (MEM, 12561056, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. All cells were cultured at 37°C with 5% CO₂.

Liu T., Zhu C., Chen X., Wu J., Guan G., Zou C., Shen S., Chen L., Cheng P., Cheng W, & Wu A. (2022). Dual role of ARPC1B in regulating the network between tumor-associated macrophages and tumor cells in glioblastoma. Oncoimmunology, 11(1), 2031499.

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Cell Culture Maintenance and Mycoplasma Screening

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The cell lines were obtained from ATTC (Manassas, VA, USA). HeLa (human cervix epithelioid carcinoma), MCF7 (human breast adenocarcinoma), HepG2 (human hepatocellular carcinoma), U87 MG (human glioblastoma), T98G (human glioblastoma), GL261 (murine glioma), LNCaP (human prostate cancer), PC-3 (human prostate cancer), and HEK-293 (human embryonic kidney) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Waltham, MA, USA), supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HIFBS, Sigma-Aldrich, St. Louis, MO, USA)), 2 mM L-glutamine, 100 μg/mL streptomycin, and 100 units/mL penicillin (Gibco, Waltham, MA, USA) at 37 °C in saturated humidity atmosphere containing 95% air and 5% CO₂. Cell line HT-29 (human colon carcinoma) was grown in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, Whaltman, MA, USA) supplemented with 10% HIFBS, 100 μg/mL streptomycin, and 100 units/mL penicillin (Gibco, Whaltman, MA, USA) at 37 °C in humidified 95% air and 5% CO₂ atmosphere. MycoAlert kit (Lonza, Norwest, Australia) was used to routinely check the presence of mycoplasma, and only mycoplasma-free cells were employed in the experiments.

Gallego-Yerga L., Ceña V, & Peláez R. (2023). Potent and Selective Benzothiazole-Based Antimitotics with Improved Water Solubility: Design, Synthesis, and Evaluation as Novel Anticancer Agents. Pharmaceutics, 15(6), 1698.

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Glioblastoma cell lines and transduction

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Human glioblastoma cell lines U87MG, LN229, murine glioma cell line GL261 and murine microglia BV2 were purchased from the American Type Culture Collection (ATCC, Manassas; Virginia, US). The primary cell line, TBD0220, was derived from a GBM patient who underwent a surgery at Hebei University Affiliated Hospital. U87, LN229 and GL261 were maintained in Dulbecco's modified Eagle's medium (DMEM, Gibco) supplemented with 10% EV-depleted fetal bovine serum (FBS). U87 and GL261 cells were transduced with lentiviruses encoding the eGFP-Cavin1 or eGFP-vCavin1 fusion protein (Genechem Co.LTD.; Shanghai, China) followed by puromycin treatment for 1 week.

Wang L., Yang C., Wang Q., Liu Q., Wang Y., Zhou J., Li Y., Tan Y, & Kang C. (2020). Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma. Theranostics, 10(15), 6674-6694.

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Cell Lines and Culture Conditions

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GL261, U251, U87, BV2 and HMC3 cells were purchased from the American Type Culture Collection (ATCC, Gaithersburg, MD, USA), and human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (MD, USA). Cell lines GL261, U251, U87, BV2 and HMC3 were cultured in DMEM (Gibco, USA) containing 10% fetal bovine serum (FBS; Invitrogen, China) and 100 U/ml penicillin/streptomycin (Gibco, USA). HUVECs were cultured in RPMI 1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, China) and 1% penicillin/streptomycin (Gibco, USA). All cells were cultured in a 37 °C humidified incubator with 5% CO₂.

Zhang Q., Zhang J., Tian Y., Zhu G., Liu S, & Liu F. (2020). Efficacy of a novel double-controlled oncolytic adenovirus driven by the Ki67 core promoter and armed with IL-15 against glioblastoma cells. Cell & Bioscience, 10, 124.

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Glioma Cell-Derived Conditioned Medium

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The murine glioma cell lines GL261 and the murine microglia cell line BV2 were obtained from the State Key Laboratory of Oncology in South China and used for this study. Mycoplasma infection was found to be absent in all cell lines tested. The GL261 cells and BV2 cells were maintained in DMEM with high glucose (Gibco BRL), 10% fetal bovine serum (FBS, HyClone Inc, Logan, UT), 100 U/mL penicillin and 100 μg/mL streptomycin, in a humidified atmosphere at 37 °C under 5% CO2. Supernatants were collected after glioma cells were cultured in an FBS‐free medium for 24 h, and then filtered with a polyethersulfone 0.22 μm membrane, serve as the glioma cell‐derived condition medium (GCM) for the usage of the in vitro experiments.

Lu J., Wang Z., He Z., Hu Y., Duan H., Liu Z., Li D., Zhong S., Ren J., Zhao G., Mou Y, & Yao M. (2023). Oligomer‐Aβ42 suppress glioma progression via potentiating phagocytosis of microglia. CNS Neuroscience & Therapeutics, 30(1), e14495.

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Culturing Glioma Cell Lines and Primary Astrocytes

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The mouse glioma cell line GL261 (KCB 200770YJ), the human malignant brain astroglioma U87MG (KCB2011101YJ) and U118 MG(KCB201302YJ) were purchased from the Kunming Cell Bank of the Chinese Academy of Sciences. Primary human astrocytes (HA) was purchased from the Sciencell Research (SanDiego, CA, USA). GL261 and U118 MG cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) containing 10% fetal calf serum (FCS; Gibco) and 1% penicillin–streptomycin (Life Technologies, Gaithersburg, MD) at 37 °C and 5% CO₂. U87 MG cells were cultured in Minimum Essential Medium (MEM) (Gibco) containing 1% non-essential amino acids (NEAA) (Gibco), 10% fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Life Technologies) at 37 °C and 5% CO₂.

Wang M., Cai Y., Peng Y., Xu B., Hui W, & Jiang Y. (2020). Exosomal LGALS9 in the cerebrospinal fluid of glioblastoma patients suppressed dendritic cell antigen presentation and cytotoxic T-cell immunity. Cell Death & Disease, 11(10), 896.

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Culturing Glioma Cell Lines

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Human glioma cell lines (U251 and U87) and a mouse glioma cell line (GL261) were obtained from the National Cancer Institute and ATCC (American Type Culture Collection). U251 and GL261 were carefully cultured in DMEM (Gibco, Thermo Scientific, MA, USA) consisting of 10% fetal bovine serum (Gibco, Thermo Scientific, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) and U87 with RPMI 1640 (Gibco, Thermo Scientific, MA, USA). All cell lines were maintained at 37 °C with 5% CO₂.

Chen L., Yan Y., Kong F., Wang J., Zeng J., Fang Z., Wang Z., Liu Z, & Liu F. (2022). Contribution of Oxidative Stress Induced by Sonodynamic Therapy to the Calcium Homeostasis Imbalance Enhances Macrophage Infiltration in Glioma Cells. Cancers, 14(8), 2036.

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Culturing Mouse Glioblastoma and Microglia

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The mouse glioblastoma cell line GL261 and mouse microglia cell line BV2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). The GL261 and BV2 cell line was cultured in Dulbecco's modifed Eagle's medium (DMEM; Gibco, Waltham, MA, USA) supplemented with 1% penicillin/streptomycin (Thermo Fisher Scienti c, Inc.) and 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA). Cells were cultured at 37 °C in a humidifed incubator containing 5% CO 2 .

Li X., Guan J., Jiang Z., Cheng S., Hou W., Yao J., & Wang Z. (2020). Microglia exosomes miR-7239-3p promoting Glioma progression by regulating the Circadian genes.

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Glioblastoma Neurosphere Cultivation and Manipulation

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All reagents were purchased from Sigma–Aldrich (St Louis, MO) unless otherwise stated. The GBM patient-derived neurosphere line HSR-GBM1A (GBM1A) was originally derived from Dr. Vescovi's group.¹⁸ (link) GFP⁺ control and UGDH knock down (KD) GBM1A cells were generated with GFP tagged UGDH shRNA as we described in Oyinlade et al.¹³ (link) Mouse glioma cell line GL261 was original purchased from American Type Culture Collection (ATCC, Manassas, VA). All cell lines are free from mycoplasma and authenticated with short tandem repeat proﬁling by Johns Hopkins Genetic Resources Core facility using Promega Gene Print 10 system (Madison, WI). GBM1A neurosphere cells were cultured in Dulbecco's modiﬁed Eagle's medium (DMEM)/F12 medium (Thermo Fisher Scientific, Waltham, MA) supplemented with epidermal growth factor (EGF) (Peprotech, Rocky Hill, NJ) and ﬁbroblast growth factor (FGF) (Peprotech). GL261 cells were cultured in Dulbecco's Minimum Essential Media (Thermo Fisher Scientific) supplemented with 10% fetal calf serum (FCS, Gemini Bio-products, West Sacramento, CA). All cells were grown at 37 °C in a humidified incubator with 5% CO_2, and passaged every 2–4 days.

Zhan D., Yalcin F., Ma D., Fu Y., Wei S., Lal B., Li Y., Dzaye O., Laterra J., Ying M., Lopez-Bertoni H, & Xia S. (2021). Targeting UDP-α-d-glucose 6-dehydrogenase alters the CNS tumor immune microenvironment and inhibits glioblastoma growth. Genes & Diseases, 9(3), 717-730.

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