MDA levels in tissues were evaluated using commercially available assay kits to determine the lipid peroxidation state (TBARS Assay Kit, item no. 10009055, Cayman Chemicals, Ann Arbor, MI, USA). The measuring principle is based on the reaction with thiobarbituric acid (TBA) in boiling water for 60 min in an acidic medium and the measurement of the absorbance of the reaction mixture at 532 nm (Ohkawa et al., 1979). A VersaMax Tunable Microplate Reader was used to measure the absorbance (Molecular Devices, San Jose, CA, USA). MDA concentrations in tissues were expressed as nmol MDA/mg protein.
In tissues, GPx and SOD activity were evaluated using ready-to-use assay kits. (RANSEL RS505, RANSOD SD125, Randox Laboratories Ltd., Crumlin, UK) by using an automated BS-240 VET Clinical Chemistry Analyser (Mindray, Shenzhen, China). The quantities of GPx and SOD in the tissue samples (stomach, small intestine, and large intestine) were expressed as U/mg protein.
In tissues, GPx and SOD activity were evaluated using ready-to-use assay kits. (RANSEL RS505, RANSOD SD125, Randox Laboratories Ltd., Crumlin, UK) by using an automated BS-240 VET Clinical Chemistry Analyser (Mindray, Shenzhen, China). The quantities of GPx and SOD in the tissue samples (stomach, small intestine, and large intestine) were expressed as U/mg protein.