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Sodium fumarate dibasic

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Sodium fumarate dibasic is a laboratory chemical compound used as a reagent in various scientific applications. It is a white, crystalline powder that is soluble in water. The core function of sodium fumarate dibasic is to serve as a chemical intermediate or a source of fumarate ions in research and analytical procedures.

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Lab products found in correlation

Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions) Malic acid, by Merck Group (1 mentions) Diaphorase from clostridium kluyveri, by Merck Group (1 mentions) Hbss solution, by Thermo Fisher Scientific (1 mentions) Amplex red 10 acetyl 3 7 dihydroxyphenoxazine, by Cayman Chemical (1 mentions) Brij 35, by Merck Group (1 mentions) Horseradish peroxidase hrp, by Merck Group (1 mentions) Riboflavin, by Merck Group (1 mentions) Sodium succinate dibasic hexahydrate, by Merck Group (1 mentions) Dl isocitric acid trisodium salt hydrate, by Merck Group (1 mentions) α ketoglutaric acid disodium salt dihydrate, by Merck Group (1 mentions) Sodium citrate, by Merck Group (1 mentions) Oligomycin, by Cayman Chemical (1 mentions) Rotenone, by Cayman Chemical (1 mentions) Norvaline, by Merck Group (1 mentions) Methyl pyruvate, by Merck Group (1 mentions) Deferoxamine mesylate, by Cayman Chemical (1 mentions) Hexadimethrine bromide, by Merck Group (1 mentions) Diethyl fumarate, by Merck Group (1 mentions) Methoxyamine hydrochloride, by Merck Group (1 mentions)

6 protocols using sodium fumarate dibasic

1

Myocardial Infarction in C57Bl/6 Mice

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Myocardial infarction (MI) of C57Bl/6 mice at age of 8 weeks was induced as previously described17 (link). Briefly, general anesthesia was performed with 1-2% isoflurane and maintained on a ventilator. The heart was exposed to a left open thoracotomy, and the left anterior descending branch (LAD) was ligated with a 6-0 polyester thread 1 mm from the normal position of the left pinna tip. The surgical wound was sutured, and animals were allowed to recover. For the fumarate supplementation, the sodium fumarate dibasic (Sigma-Aldrich, F1506) was admitted to Sham and MI group at the dose of 2 g/L through drinking water 1 day prior to the surgery and lasted 7 days after the surgery.
Wang X., Zhang X., Cao K., Zeng M., Fu X., Zheng A., Zhang F., Gao F., Zou X., Li H., Li M., Lv W., Xu J., Long J., Zang W., Chen J., Gao F., Ding J., Liu J, & Feng Z. (2022). Cardiac disruption of SDHAF4-mediated mitochondrial complex II assembly promotes dilated cardiomyopathy. Nature Communications, 13, 3947.
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2

Fumarate Hydratase Inhibitor Screening

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Recombinant human fumarate hydratase (with 6 histidine tag in the N-terminal of protein), dithiothreitol (DTT) and HBSS solution were purchased from Thermofisher Scientific (Waltham, MA). L-malate dehydrogenase from pig heart, diaphorase from clostridium kluyveri, β-NAD (NAD+), resazurin, Brij 35, sodium fumarate dibasic, malic acid and horse radish peroxidase (HRP) were purchased from Sigma (St. Louis, MO). Fumarate hydratase-IN-2 was purchased from MedChem Express (Monmouth Junction, NJ). Compound 8 was purchase from Enamine (Monmouth Junction, NJ). Amplex Red (10-Acetyl-3,7-dihydroxyphenoxazine) was purchased from Cayman Chemical (Ann Arbor, MI). Six libraries were screened, including Sytravon (a retired Pharma screening collection that contains a diversity of novel small molecules, NCATS), NPACT (NCATS Pharmacologically Active Chemical Toolbox, NCATS), NPC (NCATS Pharmaceutical Collection, NCATS),16 MIPE (Mechanism Interrogation Plate, NCATS), Kinase inhibitor library (an in-house collection of kinase inhibitors, NCATS) and LOPAC (1,280 Pharmacologically Active Compounds Sigma-Aldrich, St. Louis, MO) libraries.
Zhu H., Lee O.W., Shah P., Jadhav A., Xu X., Patnaik S., Shen M, & Hall M.D. (2019). Identification of activators of human fumarate hydratase by quantitative high-throughput screening. SLAS discovery : advancing life sciences R & D, 25(1), 43-56.
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3

A. baumannii Growth in Minimal Media

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Overnight cultures of A. baumannii were subcultured 1:50 in LB for 1 h. Cultures were then inoculated 1:100 into M9 minimal media supplemented with Vishniac’s trace metal mix with or without ZnCl2 as previously described (Nairn, et al., 2016 (link)). M9 minimal media supplemented with full Vishniac’s trace metal mix contained 36 μM Fe, 5.2 μM Mn, 3.2 μM Cu, 1.8 μM Mo, 13.4 μM Co and 27.2 μM Zn. Sodium succinate dibasic hexahydrate (Sigma) or sodium fumarate dibasic (Sigma) were used as the sole carbon source at 0.5 % (w/v). Riboflavin (Sigma) was supplemented with a final concentration of 10 μg/mL, and OD600 was monitored over time. Data are representative of at least 3 independent experiments.
Wang J., Lonergan Z.R., Gonzalez-Gutierrez G., Nairn B.L., Maxwell C.N., Zhang Y., Andreini C., Karty J.A., Chazin W.J., Trinidad J.C., Skaar E.P, & Giedroc D.P. (2019). Multi-metal restriction by calprotectin impacts de novo flavin biosynthesis in Acinetobacter baumannii. Cell chemical biology, 26(5), 745-755.e7.
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4

Extracellular Pel Activity Measurement

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Extracellular Pel activity was measured by spectrometry as previously described (Matsumoto et al. 2003 (link)). In brief, bacterial cells were cultured in LB broth supplemented with 0.1% (wt/vol) polygalacturonic acid (PGA) at 28°C for 16 h. When needed, 0.4% (wt/vol) sodium citrate, sodium pyruvate, sodium acetate, sodium fumarate dibasic, α-ketoglutaric acid disodium salt dihydrate, or DL-isocitric acid trisodium salt hydrate (Sigma-Aldrich, St. Louis, MO, U.S.A.) were applied. The OD600 of bacterial cultures was measured and normalized. Culture supernatant was collected by centrifugation of 1 ml of bacterial cultures at 13,000 × g for 2 min. The supernatant (10 μl) was added into 990 μl of the reaction buffer (0.05% [wt/vol] PGA, 0.1 M Tris-HCl [pH 8.5], and 0.1 mM CaCl2, prewarmed to 30°C). Pel activity was monitored at an absorbance at 230 nm for 3 min and calculated based on one unit of Pel activity being equal to an increase of 1 × 10 −3 OD230 in 1 min.
Yuan X., Zeng Q., Xu J., Severin G.B., Zhou X., Waters C.M., Sundin G.W., Ibekwe A.M., Liu F, & Yang C.H. (2019). Tricarboxylic Acid (TCA) Cycle Enzymes and Intermediates Modulate Intracellular Cyclic di-GMP Levels and the Production of Plant Cell Wall–Degrading Enzymes in Soft Rot Pathogen Dickeya dadantii. Molecular plant-microbe interactions : MPMI, 33(2), 296-307.
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5

Isotopic Labeling for Metabolic Tracing

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13C5-labeled glutamine (CLM-1822-H), 2H6-labeled α-ketoglutaric acid (DLM-9476), 13C4-labeled succinic acid (CLM-1571), and 15N-labeled alanine (NLM-454) were acquired from Cambridge Isotope Laboratories. HPLC-grade reagents, including methanol, chloroform, water, and acetone were acquired from Sigma. Oligomycin (Cayman Chemicals), FCCP (Cayman Chemicals), rotenone (Cayman Chemicals), antimycin A (Sigma), Seahorse XFe96 XF base media (Agilent), D-glucose (Sigma), sodium chloride (Sigma), L-glutamine (Sigma), sodium pyruvate (Sigma), α-ketoglutaric acid disodium salt (Sigma), dimethyl 2-oxoglutarate (Sigma), DBE-4 (dimethyl-succinate; Sigma), DBE-5 (dimethyl-glutarate; Sigma), 1-octyl-α-ketoglutarate (Cayman Chemicals), methyl pyruvate (Sigma), tert-butyl acetate (Sigma), sodium acetate (Sigma), L-alanine methyl ester hydrochloride (Sigma), L-alanine ethyl ester hydrochloride (Sigma), L-alanine tert-butyl ester hydrochloride (Alfa Aesar), sodium fumarate dibasic (Sigma), dimethyl fumarate (Sigma), diethyl fumarate (Sigma), norvaline (Sigma), deferoxamine mesylate (Cayman Chemicals), doxycycline hyclate (Sigma), hexadimethrine bromide (Sigma), hygromycin B (Sigma), methoxyamine hydrochloride (Sigma), and MTBSTFA + 1% TBDMSCI (Sigma).
Parker S.J., Encarnación-Rosado J., Hollinshead K.E., Hollinshead D.M., Ash L.J., Rossi J.A., Lin E.Y., Sohn A.S., Philips M.R., Jones D.R, & Kimmelman A.C. (2021). Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters. Nature Communications, 12, 4905.
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6

Shewanella oneidensis MR-1 Aerobic and Anaerobic Culturing

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Shewanella oneidensis MR‐1 (ATCC® 700550™) was aerobically and anaerobically cultured in rich Tryptic Soy Broth (TSB) media (Sigma Aldrich) or minimal media (28 mM NH4Cl, 1.34 mM KCl, 5 mM NaH2PO4, 0.7 mM Na2SO4, 1 mM MgSO4 7H2O, 20 mM PIPES [piperazine‐N,N′‐bis(2‐ethanesulfonic acid)], 52 mM NaCl, 0.2 mM CaCl2, and trace elements (1 liter of medium contains 10 mg FeCl2 4H2O, 5 mg MnCl2 4H2O, 3 mg CoCl2 4H2O, 2 mg ZnCl2, 0.5 mg Na2MoO4 4H2O, 0.2 mg H3BO3, 1 mg NiSO4 6H2O, 0.02 mg CuCl2 2H2O, 0.06 mg Na2SeO3 5H2O, and 0.08 mg Na2WO4 2H2O) containing 20 mM L‐lactate (Sigma Aldrich) and/or 20 mM Sodium fumarate dibasic (Sigma Aldrich) overnight at 30 °C under continuous shaking (250 rpm).49
Lehner B.A., Janssen V.A., Spiesz E.M., Benz D., Brouns S.J., Meyer A.S, & van der Zant H.S. (2019). Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis. ChemistryOpen, 8(7), 888-895.
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