α-Glucosidase, α-amylase, and acarbose, 2,2-diphenyl-1-picrylhydrazyl (DPPH), were from Sigma-Aldrich (St. Louis, MO, USA), naphthyl ethylenediamine dihydrochloride, gallic acid, quercetin, sodium nitrate, methanol, trisaminomethane (Tris), nitro blue tetrazolium chloride (NBT), 1,4 Dihydronicotinamide adenine dinucleotide (NADH), phenazine methosulfate (PMS), FeSO4, saffron, salicylic acid, sodium nitroprusside, sulfanilic acid reagent, glacial acetic acid, and naphthyl ethylene diamine dihydrochloride were acquired from Sigma-Aldrich (Shanghai, China), 3-(4,5-dimethylthaizol-2-yl)-2,5-diphenytetrazolium bromide (MTT) was procured from Enzo Life Science (Plymouth Meeting, PA, USA). D-Hank’s buffer, dimethyl sulfoxide (cell-culture grade), and 0.25% EDTA trypsin were obtained from (Solarbio, China); RPMI 1640 medium and fetal bovine serum were acquired from (GIBCO, USA). Microbial strains; Escherichia Coli ATCC 8739, Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 10876, and Salmonella enterica ATCC 14028 were obtained from Microbiologic (St. Cloud, Minnesota, USA).
Trisaminomethane
Manufactured by Merck Group
Sourced in United States
Trisaminomethane is a chemical compound commonly used as a buffer solution in various laboratory applications. It maintains a stable pH and helps control the acidity or basicity of solutions. The compound is widely used in biochemical, analytical, and molecular biology experiments.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Nitroblue tetrazolium chloride, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Trypsin edta, by Solarbio (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Salicylic acid, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
Sodium nitrate, by Merck Group (1 mentions)
Feso4, by Merck Group (1 mentions)
Phenazine methosulfate, by Merck Group (1 mentions)
Acarbose, by Merck Group (1 mentions)
Naphthylethylenediamine dihydrochloride, by Merck Group (1 mentions)
α glucosidase, by Merck Group (1 mentions)
Sodium nitroprusside, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Sulfanilic acid reagent, by Merck Group (1 mentions)
D hank s buffer, by Solarbio (1 mentions)
5 protocols using trisaminomethane
1
Antimicrobial and Antioxidant Assays
2
Cytotoxicity Evaluation of CBD and CBD-Capped NPs
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The following materials were used in the cytotoxicity
evaluation
of CBD and CBD-capped NPs in HaCaT cells: HaCaT cell line (human epidermal
keratinocytes; American Type Culture Collection); CBD (Endoca BV Netherlands);
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine
serum (FBS), phosphate-buffered saline (PBS) (Gibco—Thermo
Fisher Scientific, South Africa); and acetic acid, dimethyl sulfoxide
(DMSO), ethylenediaminetetraacetic acid (EDTA), fungizone, penicillin,
sodium hydroxide (NaOH), streptomycin, sulforhodamine B salt, trichloroacetic
acid (TCA), trisaminomethane (Tris), trypan blue, and trypsin (Sigma-Aldrich,
South Africa).
evaluation
of CBD and CBD-capped NPs in HaCaT cells: HaCaT cell line (human epidermal
keratinocytes; American Type Culture Collection); CBD (Endoca BV Netherlands);
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine
serum (FBS), phosphate-buffered saline (PBS) (Gibco—Thermo
Fisher Scientific, South Africa); and acetic acid, dimethyl sulfoxide
(DMSO), ethylenediaminetetraacetic acid (EDTA), fungizone, penicillin,
sodium hydroxide (NaOH), streptomycin, sulforhodamine B salt, trichloroacetic
acid (TCA), trisaminomethane (Tris), trypan blue, and trypsin (Sigma-Aldrich,
South Africa).
3
Synthesis and Characterization of PAMAM-Coated MNPs
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Ferric chloride hexahydrate (FeCl36·H2O), ferrous chloride tetrahydrate (FeCl24·H2O), ammonia solution (NH3) (32%), 3-aminopropyl trimethoxysilane (APTS) [NH2 (CH2)3-Si-(OCH3)3], methylacrylate, methanol, ethanol and ethylenediamine, phosphate buffer saline (PBS), ethylenediaminetetraacetic acid, trisaminomethane, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), polyinosinic–polycytidylic acid (Poly [I:C]), and acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA) and were used in the synthesis of PAMAM-coated MNPs. The 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazoliumhydroxide (XTT) reagent was purchased from Biological Industries (Kibbutz Beit-Haemek, Israel). DOX was kindly provided by Gulhane Military Academy (School of Medicine, Ankara, Turkey). Human serum was donated by the authors of this manuscript (Rouhollah Khodadust and Pelin Mutlu) for physiological stability studies.
4
Recombinant Human Antithrombin Purification
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Tris or Tris aminomethane and 2-mercaptoetahnol were procured from Merck Millipore (USA). Fraction V or Bovine serum albumin (BSA) and phenylmethanesulfonylfluoride (PMSF) were obtained from Sigma (Germany). rhAT reference standard was purchased from NIBSC, UK. SDS-PAGE Mini-PROTEAN three apparatus was procured from Bio-Rad Laboratories Inc., (USA). Recombinant human AT was produced in a Minifors fermentor (Infors HT, Switzerland). The Fast protein liquid chromatography (FPLC) AKTA Prime Plus system, resins (stationary phase), bioprocess glass column (BPG XK16/20) for purification, cross flow filtration (CFF) system and the hollow fiber membrane cartridge (30 kDa) were purchased from GE Healthcare Life sciences (Uppsala, Sweden). The chromatographic data were recorded and analyzed by Prime View Evaluation™ software version 5. 31. Reversed phase high pressure liquid chromatography (RP-HPLC) equipped with UV/Vis (SPD 20A Prominence) detector was acquired from Shimadzu Corp. (Kyoto, Japan). The Jupiter 300 Å C4 LC column (250 × 4.6 mm) obtained from Phenomenex (USA).
5
Tris-Egg Yolk Extender Preparation
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Tris-egg yolk extender was prepared freshly by mixing 1.363 g Tris-aminomethane (Merck, CAS Number: 108382, Darmstadt, Germany), 0.762 g citric acid (CAS Number: 77-92-9 100241-Merck Millipore), 1.5 g lactose monohydrate (Merck CAS Number: 1.07660), and 2.7 g D-(+)-raffinose pentahydrate (Merck, CAS Number: 512-69-6), 0.5 g D(-)-fructose (Merck, CAS Number: 57-48-7 104007) was dissolved in 80 mL double-distilled water and homogenized with a magnetic stirrer for 10-15 min [8 (link)]. The extender osmolarity was 295 mOsm/L (WESCOR model 5500, INC, USA). The solution was heated to 100°C for a few minutes and cooled to room temperature (26°C), after which it was mixed with 0.1 g penicillin G (Meiji Seika Co., Tokyo, Japan) and 0.1 streptomycin sulfate (Meiji). The mixture was homogenized again for 10-15 min. Egg yolk (20 mL) was dissolved in 80 mL of the mixture, centrifuged 492 × g for 15 min, the supernatant was used as an extender while the precipitate was removed [2 (link)].
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