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Phospho fak y397

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Phospho-FAK Y397 is a phosphorylated form of the Focal Adhesion Kinase (FAK) protein, specifically at the tyrosine 397 residue. This protein plays a crucial role in cellular signaling pathways.

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Lab products found in correlation

Actin, by Santa Cruz Biotechnology (1 mentions) Abcg2, by Cell Signaling Technology (1 mentions) Phospho akt s473, by Cell Signaling Technology (1 mentions) Fgfr2, by Merck Group (1 mentions) Phospho fgfr y653 654, by Cell Signaling Technology (1 mentions) Phospho kit y719, by Cell Signaling Technology (1 mentions) Hrp conjugated secondary antibodies for western blotting, by Santa Cruz Biotechnology (1 mentions) C met, by Santa Cruz Biotechnology (1 mentions) Vimentin, by Merck Group (1 mentions) N cadherin, by BD (1 mentions) E cadherin, by BD (1 mentions)

2 protocols using phospho fak y397

1

Immunoblotting Antibody Validation Protocol

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Primary antibodies used for immunoblotting were as follows: total and cleaved forms of PARP and caspase-3, phospho-KIT Y719, phospho-AKT S473, AKT, ABCG2, phospho-FGFR Y653/654 (Cell Signaling, Danvers, MA, USA), FAK, phospho-FAK Y397 (BD Biosciences, Franklin Lakes, NJ, USA), KIT (DakoCytomation), phospho-Met Tyr1230/1234/1235 (R&D Systems, Minneapolis, MN, USA), Axl, FGFR2 (Sigma), c-Met, MDR, MRP-1 and actin (Santa Cruz Biotechnology, Dallas, TX, USA). The HRP-conjugated secondary antibodies for Western blotting were purchased from Santa Cruz Biotechnology.
Boichuk S., Galembikova A., Dunaev P., Valeeva E., Shagimardanova E., Gusev O, & Khaiboullina S. (2017). A Novel Receptor Tyrosine Kinase Switch Promotes Gastrointestinal Stromal Tumor Drug Resistance. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(12), 2152.
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2

Immunoblot Analysis of Cell Adhesion Proteins

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Immunoblot assays were performed as described previously (Oh et al., 2001.). The primary antibodies used were as follows: RECK (5B11D12)6 (link), E-cadherin (610181, BD Biosciences), N-cadherin (610920, BD Biosciences), FN (610077, BD Biosciences), α-tubulin (CP06, Calbiochem), vimentin (V5255, Sigma), phospho-FAK (Y397) (611722, BD Biosciences), FAK (sc-557, Santa Cruz), integrin α5 (610633, BD Biosciences), and integrin β1 (610467, BD Biosciences).
Yuki K., Yoshida Y., Inagaki R., Hiai H, & Noda M. (2014). E-cadherin–downregulation and RECK-upregulation are coupled in the non-malignant epithelial cell line MCF10A but not in multiple carcinoma-derived cell lines. Scientific Reports, 4, 4568.
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