Cells were fixed with 4% paraformaldehyde (PFA) in PBS for 10 minutes before permeabilisation in PBS containing 0.2% Triton-X for 10 minutes at room temperature. The cells were stained with DAPI (Sigma, d9542, MO, USA) to reveal DNA in cell nuclei and TRITC-phalloidin (Sigma, p1951, MO, USA) to reveal F-actin in 3% bovine serum albumin with PBS + 0.1% Tween-20. Cells were stained using anti-pMLC (S19) (Cell signaling technology #3675) or anti-pERM antibody (Cell signaling technology #3141) followed by appropriate Alexafluor-conjugated secondary antibodies (Invitrogen). PDPN-V5 was stained using anti-V5 FITC-conjugated antibody (Invitrogen R963-25). ARP2/3 was stained using Anti-p34-Arc/ARPC2 antibody (07–227 Millipore).
Anti p34 arc arpc2 antibody
Manufactured by Merck Group
The Anti-p34-Arc/ARPC2 antibody is a laboratory reagent used in scientific research. It is a tool that allows researchers to detect and study the p34-Arc/ARPC2 protein, which is a key component of the Arp2/3 complex involved in actin cytoskeleton regulation. The antibody can be utilized in various experimental techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation, to identify and analyze the p34-Arc/ARPC2 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti v5 fitc conjugated antibody, by Thermo Fisher Scientific (2 mentions)
Anti pmlc s19, by Cell Signaling Technology (2 mentions)
Anti perm antibody, by Cell Signaling Technology (2 mentions)
Phalloidin tritc, by Merck Group (2 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
2 protocols using anti p34 arc arpc2 antibody
1
Cytoskeletal Protein Localization Assay
2
Cytoskeletal Protein Localization Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed with 4% paraformaldehyde (PFA) in PBS for 10 minutes before permeabilisation in PBS containing 0.2% Triton-X for 10 minutes at room temperature. The cells were stained with DAPI (Sigma, d9542, MO, USA) to reveal DNA in cell nuclei and TRITC-phalloidin (Sigma, p1951, MO, USA) to reveal F-actin in 3% bovine serum albumin with PBS + 0.1% Tween-20. Cells were stained using anti-pMLC (S19) (Cell signaling technology #3675) or anti-pERM antibody (Cell signaling technology #3141) followed by appropriate Alexafluor-conjugated secondary antibodies (Invitrogen). PDPN-V5 was stained using anti-V5 FITC-conjugated antibody (Invitrogen R963-25). ARP2/3 was stained using Anti-p34-Arc/ARPC2 antibody (07–227 Millipore).
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