Hsa mirna mimics

Hsa-miRNA mimics and their cognate control RNAs were purchased from Ribobio (Guangzhou, China). Full-length ZFX cDNA (GenBank accession number NM_001178084.1) was cloned into a GV143 expression vector (Genechem, Shanghai, China). An empty vector was used as a control. Transfection was performed using Lipofectamine 2000 transfection reagent (Invitrogen) according to the manufacturer's instructions. Typically, a 50 nmol/L concentration of miRNA mimics was used for transfection of the RNA oligonucleotides. For plasmid transfections, 3 μg of DNA was used in a 6-well plate, and G418 (200 μg/mL) was added 24 h after transfection. For rescue experiments, 1 μg of plasmid was used in a 6-well plate. The sequences of the miRNAs used are listed in Supplementary Table S1.

Hsa-miRNA mimics, hsa-miRNA inhibitors and their cognate control RNAs were purchased from Riobio (Guangzhou, China). Lentivirus-miR-143-3p and lentivirus-miR-NC were purchased from Genomeditech (Shanghai, China). Additionally, an ITGA6 overexpression plasmid was used for the rescue experiments. The miRNA mimics, miRNA inhibitors and plasmids were transfected into NOZ and GBC-SD cells using Lipofectamine 2000 transfection reagent (Invitrogen, USA). Total RNA and protein were collected 48 h after transfection.