Harvested HeLa cells were lysed in lysis-buffer (10 mM HEPES, 1.5 mM MgCl2, 300 mM sucrose, 10 mM KCl, 0.5% NP40, Roche Complete Protease Inhibitor Cocktail (Roche, Vienna, Austria), 5 mM DTT prepared freshly from a 1 M stock) on ice for at least one hour. Proteins were separated by SDS/PAGE, transferred to a nitrocellulose membrane and incubated with the relevant antibodies: cleaved Caspase-3 ([Asp175] Cell Signalling), Parp (#9542, Cell Signalling), Hsp 90 α/β (sc-13119, Santa Cruz Biotechnology). Treatments: NoA compound (6.7 μM) for 12/24/36/48 h and Staurosporine 1 μM for 4 h (♯S5921, Sigma), co-treatment: pan-caspase inhibitor Q-VD-OPh (10 μM, ♯SML0063, Sigma) was added one hour before.
Sml0063
Manufactured by Merck Group
SML0063 is a laboratory equipment product. It is a precision instrument designed for scientific applications. The core function of SML0063 is to perform specific tasks in a controlled and accurate manner within a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Q vd oph, by Merck Group (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Hsp90 α β, by Santa Cruz Biotechnology (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
S5921, by Merck Group (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Bradford dye reagent, by Bio-Rad (1 mentions)
Phosphatase inhibitor, by Roche (1 mentions)
Complete edta free protease inhibitor, by Roche (1 mentions)
Sotrastaurin, by Selleck Chemicals (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Chir 124, by Selleck Chemicals (1 mentions)
Pf 477736, by Selleck Chemicals (1 mentions)
D5879, by Merck Group (1 mentions)
3 protocols using sml0063
1
Apoptosis Signaling Pathway Characterization
2
Comprehensive Reagents and Inhibitors Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysotracker Red DND-99 (L7528) was from ThermoFisher Scientific. Antimycin A (A8674), DFP (379409), DMOG (D3695), SGC-GAK-1 (GAKi, SML2202), SGC-GAK-1N (GAKc, SML2203) and QVD-OPh (SML0063) were from Sigma Aldrich. Bafilomycin A1 (BML-CM110), CCCP (BML-CM124) were from Enzo Life Sciences. Enzastaurin (S1055), oligomycin A (S1478), and sotrastaurin (S2791) were from Selleckchem. MRT68921 (1190379-70-4) and VPS34-IN1 (1383716-33-3) were from Cayman Chemical. IVAP1966 (12 g) and IVAP1966 (12i) were gratefully received from the lab of Prof. Piet Herdewijn29 . HY-19764 was gratefully received from the structural genomics consortium30 (link). Bradford reagent dye (#5000006) was from Bio-Rad. 1,4-dithiothreitol (DTT, #441496P) was from VWR. Complete EDTA-free protease inhibitors (#05056489001) and phosphatase inhibitors (#04906837001) were from Roche.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!