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Mab414

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MAB414 is a mouse monoclonal antibody that recognizes human Interleukin-6 (IL-6). IL-6 is a pleiotropic cytokine involved in the regulation of various cellular processes.

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Lab products found in correlation

Mab005, by R&D Systems (6 mentions) Ab9726, by Abcam (1 mentions) Ab9741, by Abcam (1 mentions) Enbrel, by Amgen (1 mentions) Actemra, by Roche (1 mentions) Lipopolysaccharides (lps), by R&D Systems (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Be0088, by BioXCell (1 mentions) Mab406, by R&D Systems (1 mentions) 7666 mb 005 cf, by R&D Systems (1 mentions) 410 mt 010 cf, by R&D Systems (1 mentions) Af449, by R&D Systems (1 mentions) Ab 108 c, by R&D Systems (1 mentions) Azd1480, by Selleck Chemicals (1 mentions) Sc 2027, by Santa Cruz Biotechnology (1 mentions) Polyclonal hamster igg, by BioXCell (1 mentions) Anti il 1β antibody clone b122, by BioXCell (1 mentions)

10 protocols using mab414

1

Colorectal Cancer Induction and Anti-G-CSF Treatment

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Female C57Bl/6 (B6) mice from Harlan Laboratories (Houston, TX) were housed under pathogen free conditions. Under approval of the UNM IACUC, at 6 weeks of age the mice received one 12.5mg/kg intraperitoneal (IP) injection of AOM. During days 5–10 and 26–31 after injection mice received 2.5% DSS in drinking water and on days 47–51 they received 2% DSS. At day 54, mice were administered either 25 μg of IgG1 or anti-G-CSF antibody (R&D Systems MAB005 and MAB414) via IP injection 3 times a week for 3 weeks and sacrificed on Day 80 using CO2. Neoplasms were counted and measured L x W (mm) under a dissecting microscope. Colon tissues were divided for flow cytometry, quantitative real time PCR (qRT-PCR) and histological examination.
Morris K.T., Castillo E.F., Ray A.L., Weston L.L., Nofchissey R.A., Hanson J.A., Samedi V.G., Pinchuk I.V., Hudson L.G, & Beswick E.J. (2015). Anti-G-CSF treatment induces protective tumor immunity in mouse colon cancer by promoting NK cell, macrophage and T cell responses. Oncotarget, 6(26), 22338-22347.
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2

Inhibition of Cytokine-Induced Epithelial Growth

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Cytokines previously reported to be able to induce EG (30 (link), 31 (link)) were inhibited by injection of antibody or biological inhibitors. Anakinra [Kineret from Sobi DIN 02245913; anti-IL-1 at 25 μg/g mouse as used in (46 (link))], etanercept [Enbrel from Amgen, DIN 02242903; anti–TNF-α at 15 μg/g mouse as used in (47 (link))], tocilizumab [Actemra from Roche, DIN 02350092; anti-IL-6R at 5 μg/g mouse as used in (48 (link))], anti–IL-3 (aIL3 from Abcam, ab9726; at 2.5 μg/g mouse as recommended by the product sheet], anti–G-CSF (from R&D Systems, MAB414; 10 μg per injection as used in (49 (link))], and anti–GM-CSF (from Abcam, ab9741; at 10 μg/g mouse as recommended by the product sheet] were each administered in 40-μl intraperitoneal doses to separate mice 12 hours before vaccination, at vaccination, and at 24 and 48 hours after vaccination until euthanasia at 72 hours after vaccination for flow cytometry of splenocytes.
Brook B., Harbeson D.J., Shannon C.P., Cai B., He D., Ben-Othman R., Francis F., Huang J., Varankovich N., Liu A., Bao W., Bjerregaard-Andersen M., Schaltz-Buchholzer F., Sanca L., Golding C.N., Larsen K.L., Levy O., Kampmann B., Tan R., Charles A., Wynn J.L., Shann F., Aaby P., Benn C.S., Tebbutt S.J., Kollmann T.R, & Amenyogbe N. (2020). BCG vaccination–induced emergency granulopoiesis provides rapid protection from neonatal sepsis. Science translational medicine, 12(542), eaax4517.
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3

Anti-G-CSF Therapy for Bone Marrow Recovery

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Mice were treated i.p. with 10μg of anti-G-CSF (R&D Systems, Cat# MAB414) or isotype control IgG (R&D Systems, Cat# MAB005) in a 100μl volume of saline 12 hours prior to injury. Antibody dosing was repeated at the time of injury and daily on days 1 through 6 post injury for a total of 8 doses. Bone marrow cells and blood were harvested on day 7 post injury.
Gardner J.C., Noel J.G., Nikolaidis N.M., Karns R., Aronow B.J., Ogle C.K, & McCormack F.X. (2014). G-CSF Drives a Post Traumatic Immune Program that Protects the Host from Infection. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2405-2417.
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4

Colorectal Cancer Mouse Model with Anti-GCSF Treatment

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Host mice received an intraperitoneal injection of azoxymethane (AOM; 10 mg/kg). 5 days later, mice received dextran sulfate sodium (DSS) at 2.5% ad libitum in drinking water for 7 days, followed by a second 5-day cycle at 2.5% and a third DSS cycle (5 days, 2.0%). DSS cycles were interspaced with a recovery cycle with sterile drinking water for 14 days. At the end of the third recovery cycle, mice were bled and received 25 μg of either isotype (IgG1, R&D Systems) or anti-GCSF (MAB414, R&D Systems) antibody intraperitoneally three times a week for 3 weeks. Colons were harvested, cleaned, washed and tumor load was assessed under a dissection microscope. Tissues were processed to a single-cell suspension as described previously (31 (link)). Each in vivo study contained at least four replicates and the study was repeated three times.
Matos I., Barvalia M., Chehal M.K., Robertson A.G., Kulic I., Silva J.A., Ranganathan A., Short A., Huang Y.H., Long E., Priatel J.J., Dhanji S., Nelson B.H., Krebs D.L, & Harder K.W. (2023). Tumor-derived GCSF Alters Tumor and Systemic Immune System Cell Subset Composition and Signaling. Cancer Research Communications, 3(3), 404-419.
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5

Systemic Infection and Inflammation Protocol

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For systemic infection, mice were intraperitoneally injected with 1 × 106E. coli (Cat. no. CW0808S; CWBIO) once every 2 d for 9 d. For LPS-induced systemic inflammation, WT and TLR4−/− mice were intraperitoneally injected with 0.5 mg/kg LPS (Cat. no. L2880; Sigma-Aldrich) twice daily for 9 d. To neutralize G-CSF, LPS-treated mice were intravenously injected with anti–G-CSF neutralizing antibody (Cat. no. MAB414; RD Systems) or rat IgG1 isotype control (Cat. no. MAB005; RD Systems) on days 2, 5, and 8 during LPS administration.
Jing W., Guo X., Qin F., Li Y., Wang G., Bi Y., Jin X., Han L., Dong X, & Zhao Y. (2020). G-CSF shifts erythropoiesis from bone marrow into spleen in the setting of systemic inflammation. Life Science Alliance, 4(1), e202000737.
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6

MC38 Tumor Growth Inhibition

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Five to eight-week-old, male and female C57Bl/6J mice were obtained from Jackson Laboratories (RRID:IMSR_JAX:000664). On Day 0, an intraperitoneal (IP) injection of 105 MC38 cells was performed. Mice were all euthanized on Day 14 after having received 5 total treatments of 25 μg/mouse αGCSF (R&D, MAB414) or 25 μg/mouse IgG1 isotype control (R&D, MAB005) every other day, beginning Day 3.
Grohovaz F., Bossi M., Pezzati R., Meldolesi J, & Tarelli F.T. (1996). High resolution ultrastructural mapping of total calcium: electron spectroscopic imaging/electron energy loss spectroscopy analysis of a physically/chemically processed nerve-muscle preparation.. Proceedings of the National Academy of Sciences of the United States of America, 93(10), 4799-4803.
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7

Anti-G-CSF Treatment for Injury

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Mice received 10 μg of anti-G-CSF (catalog no. MAB414; R&D Systems) or isotype control IgG (catalog no. BE0088; Bio X cell) by the i.p. route in a 100μL volume of saline 12 hours before injury, at the time of injury, and daily until the day before harvest.
Noel J.G., Ramser B.J., Cancelas J.A., McCormack F.X, & Gardner J.C. (2017). Thermal injury of the skin induces G-CSF-dependent attenuation of EPO-mediated STAT signaling and erythroid differentiation arrest in mice. Experimental hematology, 56, 16-30.
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8

Modulating Leukocyte Homeostasis in Mice

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Fut7−/− mice were intravenously injected with control rIgG, a monoclonal anti–G-CSF (MAB414; R&D Systems) antibody for 3 d (daily dose of 50 µg/mouse) injected at ZT13, or monoclonal anti–GM-CSF (MAB415; R&D) injected once a day at ZT13 for two consecutive days. Leukocyte counts and blood CFU-C were analyzed the next day at ZT5 and ZT13.
For IL-23 or IL-17 blockade, Fut7−/− mice were intravenously injected with 200 µg isotype control rIgG, a monoclonal anti–IL-23 antibody (MAB1887, clone 320234; R&D Systems) or a monoclonal anti–IL-17 (MAB421, clone 50104; R&D Systems) antibody at ZT13. Leukocyte counts and blood CFU-C were analyzed the next day at ZT5.
To induce defective migration in various mutant mice, we injected mice intravenously with 25 µg of control rIgG or anti–P- and anti–E-selectin antibodies (clones RB40.34 and 9A9 from BioXcell, respectively) at days −5, −3, and −1 at ZT2.
Casanova-Acebes M., Nicolás-Ávila J.A., Li J.L., García-Silva S., Balachander A., Rubio-Ponce A., Weiss L.A., Adrover J.M., Burrows K., A-González N., Ballesteros I., Devi S., Quintana J.A., Crainiciuc G., Leiva M., Gunzer M., Weber C., Nagasawa T., Soehnlein O., Merad M., Mortha A., Ng L.G., Peinado H, & Hidalgo A. (2018). Neutrophils instruct homeostatic and pathological states in naive tissues. The Journal of Experimental Medicine, 215(11), 2778-2795.
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9

Cytokine Regulation of Cell Signaling

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Cells were treated with 0.1 or 1 ng/mL mouse (400-ML-005/CF, R&D Systems) or human (200-LA-002/CF, R&D Systems) IL-1α, 1 ng/mL mouse IL-1β (401-ML-005/CF, R&D Systems), 10 ng/mL mouse TNF-α (410-MT-010/CF, R&D Systems), 20 ng/mL mouse (7666-MB-005/CF, R&D systems) or human TGF-β1 (T7039-2UG, Sigma), 500 nM JAK inhibitor AZD1480 (S2162, Selleck Chem), 30 μM IKK-β inhibitor ML102B, 1 μM A83-01 (2939, Tocris Bioscience), 3 μg/mL IL-1α neutralizing antibody (MAB4001, R&D Systems) or an IgG control (400902, Biolegend), 5 μg/mL TNF-α neutralizing antibody (11969S, Cell signaling) or an IgG control (sc-2027, Santa Cruz), 3.4 μg/mL LIF neutralizing antibody (AF449, R&D Systems) or an IgG control (AB-108-C, R&D), 3.8 μg/mL G-CSF (MAB414, R&D Systems) or IL-6 (MAB406, R&D Systems) neutralizing antibodies or an IgG control (MAB005, R&D).
Biffi G., Oni T.E., Spielman B., Hao Y., Elyada E., Park Y., Preall J, & Tuveson D.A. (2018). IL-1-induced JAK/STAT signaling is antagonized by TGF-β to shape CAF heterogeneity in pancreatic ductal adenocarcinoma.. Cancer discovery, 9(2), 282-301.
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10

Modulating Immune Response in Polytrauma

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For G-CSF blockade experiments, mice were injected i/p with 25 μg anti-mouse G-CSF antibody (MAB414, R&D Systems) or rat IgG1 isotype control antibody (MAB005, R&D Systems) at 15 hours before the polytrauma procedure. A second dose of 25 μg antibody was given subcutaneously immediately before polytrauma. Bone marrow progenitor populations were analyzed at 24h after polytrauma. IL-1α and IL-1β blockade experiments were performed by injection (at 15 hours prior to polytrauma and at time of injury) of 150 μg anti-IL-1α antibody, clone ALF-161, and/or 150 μg anti-IL-1β antibody clone B122 (both from BioXCell). Control mice received polyclonal hamster IgG) (BioXCell). For IL-1R blockade experiments, mice were treated with 3 mg recombinant IL-1RA (Anikinra, SOBI pharmaceuticals) injected subcutaneously 1 hour before polytrauma; control animals received an equivalent volume of normal saline. In some experiments, 3 doses of IL-1RA were administered, at times −16 h, 0 h, and 8 h of the polytrauma procedure.
Fuchs A., Monlish D.A., Ghosh S., Chang S.W., Bochicchio G.V., Schuettpelz L.G, & Turnbull I.R. (2019). Trauma Induces Emergency Hematopoiesis through IL-1/MyD88 dependent production of G-CSF. Journal of immunology (Baltimore, Md. : 1950), 202(10), 3020-3032.
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