All reagents and deuterated solvents used for reactions and spiking experiments were purchased from Sigma-Aldrich and were used without further purification. All photochemical reactions were carried out in Norell Suprasil quartz NMR tubes purchased from Sigma-Aldrich using Hg lamps with principal emission at 254 nm in a Rayonet photochemical chamber reactor RPR-200, acquired from The Southern New England Ultraviolet Company. StarLab is an in-house constructed photoreactor that delivers broadband UV-Vis (from 220 nm to ~750 nm by using water as an optical filter) irradiation to a sample from a 75W xenon lamp manufactured by Horiba38 . A Mettler Toledo SevenEasy pH Meter S20 was used to monitor the pH, and degassed H2O or D2O was achieved by four rounds of freeze-pump-thaw cycling. 1H-, and 13C-nuclear magnetic resonance (NMR) spectra were acquired using a Bruker Ultrashield 400 Plus or Bruker Ascend 400 operating at 400.1, and 100.6 MHz, respectively. Samples consisting of H2O/D2O mixtures were analyzed using HOD suppression to collect 1H-NMR data. Chemical shifts (δ) are shown in ppm. Coupling constants (J) are given in Hertz and the notations s, d, t represent the multiplicities singlet, doublet, and triplet. The conversion yields were determined by relative integrations of the signals using a known amount of acetamide as internal reference in the 1H-NMR spectrum.
Seveneasy ph meter s20
Manufactured by Mettler Toledo
Sourced in United States, Switzerland, France
The SevenEasy pH Meter S20 is a compact and user-friendly pH meter designed for routine pH measurement in laboratories. It features a large, easy-to-read display and simple one-button operation. The meter is equipped with automatic temperature compensation and is compatible with a wide range of pH electrodes.
Automatically generated - may contain errors
Lab products found in correlation
Ascend 400, by Bruker (2 mentions)
Deuterated solvent, by Merck Group (2 mentions)
Norell suprasil quartz nmr tubes, by Merck Group (2 mentions)
Rayonet photochemical chamber reactor rpr 200, by Merck Group (2 mentions)
Ultrashield 400 plus, by Bruker (2 mentions)
Milli q reference water purification system, by Merck Group (2 mentions)
Inlab microelectrode, by Mettler Toledo (2 mentions)
Inlab routine pro ph electrode, by Mettler Toledo (2 mentions)
Inlab expert pro ism ph sensor, by Mettler Toledo (2 mentions)
Spectramax plus 384 microplate reader, by Molecular Devices (1 mentions)
Parafilm, by Amcor (1 mentions)
Titramax 101 orbital shaker, by Heidolph (1 mentions)
Inlab micro pro ism ph electrode, by Mettler Toledo (1 mentions)
Allegra 64r centrifuge, by Beckman Coulter (1 mentions)
No 4 filter paper, by Cytiva (1 mentions)
Whatman, by Cytiva (1 mentions)
Ag245, by Mettler Toledo (1 mentions)
Lumistox 300, by HACH (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Protease inhibitor cocktail tablet, by Roche (1 mentions)
22 protocols using seveneasy ph meter s20
1
Photochemical Reactions and NMR Analysis
2
Ultrapure Water Preparation and Measurement
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Ultrapure water was taken from a Merck Millipore (Darmstadt, Germany) Milli-Q Reference water purification system. Weighing was performed on a Sartorius (Göttingen, Germany) Research R180D-*D1 or Cubis® Advanced MCA225S-2S00-I analytical balance. Adjustment of pH values was done with a SevenEasy pH meter S20 from Mettler Toledo (Columbus, OH, USA).
3
Urine Nitrogen Preservation Procedure
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Five urine samples were selected from 10 samples of Exp. 2 to obtain variable nitrogen concentrations of 0.12, 0.26, 0.53, 0.61, and 0.94 g/100 mL. The pH changes of each 100 mL of urine samples were measured every addition of 0.2 mL of 6 N HCl using a pH meter (SevenEasy pH Meter S20; Mettler Toledo, Columbus, OH, USA).
The NLIN procedure (SAS Inst. Inc., USA) was employed to develop exponential equations for estimating urine pH by the volume of added HCl in each urine sample with various nitrogen concentrations. An equation for determining the minimum amount of 6 N HCl for nitrogen preservation in urine was generated by the REG procedure of SAS (SAS Institute, 2012) with 6 N HCl concentrations in urine as a dependent variable and nitrogen concentrations in urine as an independent variable. A plastic container was an experimental unit and statistical significance was declared at alpha less than 0.05. Additionally, the amounts of 6 N HCl required for nitrogen preservation were calculated based on actual daily nitrogen excretion data from 9 published experiments and 8 unpublished experiments conducted in our laboratory.
The NLIN procedure (SAS Inst. Inc., USA) was employed to develop exponential equations for estimating urine pH by the volume of added HCl in each urine sample with various nitrogen concentrations. An equation for determining the minimum amount of 6 N HCl for nitrogen preservation in urine was generated by the REG procedure of SAS (SAS Institute, 2012) with 6 N HCl concentrations in urine as a dependent variable and nitrogen concentrations in urine as an independent variable. A plastic container was an experimental unit and statistical significance was declared at alpha less than 0.05. Additionally, the amounts of 6 N HCl required for nitrogen preservation were calculated based on actual daily nitrogen excretion data from 9 published experiments and 8 unpublished experiments conducted in our laboratory.
4
Determination of Sample pH
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The pH of sample was determined by homogenizing 2 g of samples with 20 mL of distilled water using a homogenizer. The pH of suspension was recorded using a combined glass electrode with a digital pH meter (SevenEasy pH meter S20, Mettler Toledo, Schwerzenbach, Switzerland). Triplicate determinations on each treatment were performed.
5
Measuring pH and Temperature of Solutions
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The pH of PBS or PAP was measured using a SevenEasy™ pH meter S20 fitted with an InLab® Microelectrode (Mettler Toledo, Viroflay, France.). The temperature of PBS or PAP was measured (within 5 s after plasma treatment for PAP) with a thermocouple that was placed inside the solution.
6
pH Measurement of Buffered Solutions
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The pH of untreated and treated buffered solutions was taken using a SevenEasy™ pH meter S20 fitted with a InLab® Micro electrode (Mettler Toledo).
7
Photochemical Synthesis under Inert Conditions
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Reagents and solvents were bought from Sigma-Aldrich, Alfa Aesar and Santa Cruz Biotechnology and were used without further purification. Photochemical reactions were carried out using a Rayonet RPR-200 photochemical reactor chamber (Hg bulbs with a principle emission of 254 nm) in Spectrosil quartz cuvettes. A Mettler Toledo SevenEasy pH Meter S20 was used to monitor pH, and deoxygenation of solutions was achieved by sparging argon through the solution for 20 min. 1H and 13C NMR spectra were acquired using a Bruker Ultrashield 400 Plus machine (at 400.1 and 100.6 MHz, respectively). Samples consisting of H2O/D2O mixtures were analysed using HOD suppression to collect 1H NMR data. The notations s, d and br s represent singlet, doublet and broad signal and chemical shifts (δ) are given in ppm. Low resolution mass spectrometry was performed using an Agilent Technologies 1200 series/6130 Quadrupole LC/MS.
8
Photochemical Reactions and NMR Analysis
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All reagents and deuterated solvents used for reactions and spiking experiments were purchased from Sigma-Aldrich and were used without further purification. All photochemical reactions were carried out in Norell Suprasil quartz NMR tubes purchased from Sigma-Aldrich using Hg lamps with principal emission at 254 nm in a Rayonet photochemical chamber reactor RPR-200, acquired from The Southern New England Ultraviolet Company. StarLab is an in-house constructed photoreactor that delivers broadband UV-Vis (from 220 nm to ~750 nm by using water as an optical filter) irradiation to a sample from a 75W xenon lamp manufactured by Horiba38 . A Mettler Toledo SevenEasy pH Meter S20 was used to monitor the pH, and degassed H2O or D2O was achieved by four rounds of freeze-pump-thaw cycling. 1H-, and 13C-nuclear magnetic resonance (NMR) spectra were acquired using a Bruker Ultrashield 400 Plus or Bruker Ascend 400 operating at 400.1, and 100.6 MHz, respectively. Samples consisting of H2O/D2O mixtures were analyzed using HOD suppression to collect 1H-NMR data. Chemical shifts (δ) are shown in ppm. Coupling constants (J) are given in Hertz and the notations s, d, t represent the multiplicities singlet, doublet, and triplet. The conversion yields were determined by relative integrations of the signals using a known amount of acetamide as internal reference in the 1H-NMR spectrum.
9
ELISA Assay Optimization and Standardization
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All ELISAs were performed in transparent 96-well f-bottom high-binding polystyrene microplates from Greiner Bio-One (Frickenhausen, Germany). Wells were filled by use of Eppendorf Research® pro multichannel pipettes and dilutions of components were made with Research® plus piston stroke pipettes from Eppendorf (Hamburg, Germany). For incubation, microplates were sealed with Parafilm® from Bemis (Neenah, WI, USA) and shaken on a Titramax 101 orbital shaker from Heidolph Instruments (Schwabach, Germany). Washing steps were performed on a Microplate Washer 405 LS from BioTek Instruments (Winooski, VT, USA) and absorbance measurements on a SpectraMax Plus 384 microplate reader from Molecular Devices (San José, CA, USA).
Pure water was taken from a Merck Millipore Milli-Q Reference water purification system. Weighing was performed on a Sartorius (Göttingen, Germany) Research R180D-*D1 analytical balance. Measurements of pH values were carried out with a SevenEasy pH meter S20 from Mettler Toledo (Columbus, OH, USA).
Pure water was taken from a Merck Millipore Milli-Q Reference water purification system. Weighing was performed on a Sartorius (Göttingen, Germany) Research R180D-*D1 analytical balance. Measurements of pH values were carried out with a SevenEasy pH meter S20 from Mettler Toledo (Columbus, OH, USA).
10
pH Measurement of Sol-Gel Transition
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The pH of the sols was measured before and after gelation using a SevenEasy pH meter S20 with an InLab Micro Pro-ISM pH electrode (Mettler-Toledo GmbH, Gießen, Germany).
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