Levels of selected cytokines in culture supernatant or serum were analyzed by cytokine specific ELISA as described previously (44 (link)). All recombinant murine (rm) cytokines, antibodies specific for murine cytokines and recombinant human cytokines were purchased from BD Biosciences (San Diego, CA).
Recombinant human cytokines
Manufactured by BD
Sourced in United States
Recombinant human cytokines are proteins produced through recombinant DNA technology. They are used as research tools in various scientific and medical applications.
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Lab products found in correlation
3 protocols using recombinant human cytokines
1
Cytokine Quantification by ELISA
2
Quantification of Cytokine Secretion in MDM Cultures
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Supernatants obtained on day 7 of the MDM culture were collected and the concentration of TNF, IL-10 and IL-12 was measured by the following matched mAbs pairs for ELISA (BD Pharmingen): for TNF-MAb1 (capture) and MAb11 (detection), for IL-10-JES3-9d7 (capture) and JES-12G8 (detection) and for IL-12p40/p70-C8.3 (capture) and C8.6 (detection). Recombinant human cytokines (all from BD Pharmingen) were used as standards. Tests were performed according to the manufacturer’s protocol and results were obtained using the ELISA reader (BioTek Instruments, Vinooski, VT) at 492 vs 630 nm wavelength. Detection level for TNF was 20 pg/ml, and 10 pg/ml for IL-10, IL-12p40/p70.
3
Flow Cytometry Analysis of Monoclonal Antibodies
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All monoclonal antibodies, recombinant human cytokines, and Phosflow™ reagents used in this study were procured from BD Biosciences (CA, USA). Flow cytometric data acquisition was performed on a BD LSR II (BD Biosciences, CA, USA) at the BD-CAF Flow Cytometry Centre at Stellenbosch University, Tygerberg Medical Campus.
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