Agilent human 4 180k lncrna and mrna microarrays

The Agilent Human 4 × 180K lncRNA and mRNA Microarrays (Agilent, Santa Clara, CA, USA) were performed on 12 IA tissues and 12 STAs by Gene Expression Hybridization Kit (Agilent, Santa Clara, CA, US) according to the manufacturer's instructions. The Affymetrix Human Genome U133 GeneChip Microarrays (Affymetrix, Santa Clara, California) were performed on 15 IA tissues and 15 STAs according to the manufacturer's instructions. We selected several genes randomly and examined their expression levels with quantitative real-time polymerase chain reaction (qRT-PCR). The qRT-PCR results matched well with the microarray data. The microarray data can be obtained at the Gene Expression Omnibus (GEO) database (GSE75436;http://www.ncbi.nlm.nih.gov/geo). Analyses of the arrays were performed using R software (version 3.2.3).
The differentially expressed lncRNAs and mRNAs were filtered by at least P < 0.05 and false discovery rate (FDR) < 0.05. To avoid the factors of IA rupture, we compared the differentially expressed lncRNAs and mRNAs between ruptured IA and unruptured IA with STAs, respectively (Figure 1).

The Agilent Human 4 × 180 K lncRNA and mRNA Microarrays (Agilent, Santa Clara, CA, USA) were performed using a Gene Expression Hybridization Kit (Agilent, Santa Clara, CA, US) according to the manufacturer’s instructions. Slides were washed in staining dishes with a Gene Expression Wash Buffer Kit (Agilent, Santa Clara, CA, USA) and scanned by an Agilent Microarray Scanner (Agilent, Santa Clara, CA, USA) with default settings according to the manufacturer’s instructions. Raw data were normalized by Quantile algorithm using Gene Spring Software 12.6 (Agilent Technologies). The differentially expressed lncRNAs and mRNAs were identified by using R software (version 3.2.3) with the samr package53 (link).