The chemical structures of samples were measured by X-ray photoelectron spectra (XPS, Axis Supra, Kratos), proton nuclear magnetic resonance (1H NMR, AVIII HD 600 spectrometer, Bruker) and Fourier transform infrared spectroscopy (FT-IR, VERTEX70 spectrometer, Bruker). DOX concentration was calculated by ultraviolet spectrophotometer using UV-721G (Jingke). The morphologies of samples were visualized by transmission electron microscopy using Hitachi-HT7700. The zeta potential and particle size distribution were analyzed by dynamic light scattering using Malvern Zetasizer Nano ZS90.
Aviii hd 600 spectrometer
Manufactured by Bruker
Sourced in United States
The AVIII HD 600 spectrometer is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for advanced analytical applications. It features a 600 MHz superconducting magnet and provides high-resolution, high-sensitivity NMR data for structural analysis and characterization of chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Axis supra, by Shimadzu (1 mentions)
Vertex 70 spectrometer, by Bruker (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Ht7700, by Hitachi (1 mentions)
Nicolet 5700 spectrometer, by Thermo Fisher Scientific (1 mentions)
Dd2 500 spectrometer, by Agilent Technologies (1 mentions)
J 815 spectropolarimeter, by Jasco (1 mentions)
V 650 spectrophotometer, by Jasco (1 mentions)
P 2000 polarimeter, by Jasco (1 mentions)
Silica gel, by Qingdao Marine Chemical (1 mentions)
Mci gel chp 20p, by Mitsubishi Chemical Corporation (1 mentions)
Capcell pak mg 2 c18 column, by Shiseido (1 mentions)
4 protocols using aviii hd 600 spectrometer
1
Comprehensive Chemical Characterization
2
Pectin NMR Spectroscopy Analysis
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13C NMR were recorded and operated at 125 MHz, 343 K, with 23,000 scans on a Bruker AVIIIHD600 spectrometer. The pectin samples (75 mg) were dissolved in deuterium oxide (1.5 mL, 50 mg/mL) and allowed to be mixed at 40 °C overnight. The data were processed by MestReNova x64 software version 14.3.1.31739.
3
Comprehensive Chemical Characterization
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Melting points were determined on an XT5B melting point apparatus (Beijing Keyi Electric Light Instrument Factory, Beijing, China) and were uncorrected. Optical rotations were measured with a P-2000 polarimeter (Jasco, Tokyo, Japan). ECD spectra were recorded at room temperature with a J-815 spectropolarimeter (Jasco). UV spectra were collected in MeOH on a V-650 spectrophotometer (Jasco). IR spectra were recorded on a Nicolet 5700 spectrometer (Thermo, Madison, WI, USA) by the FT-IR transmission electron microscopy method. 1H- and 13C-NMR spectra were acquired using a DD2-500 spectrometer (Agilent, Santa Clara, CA, USA) or an AVIIIHD 600 spectrometer (Bruker, Billerica, MA, USA). HRESIMS were recorded on a 1200 series LC/6520 quadrupole time of flight (QTOF) spectrometer (Agilent). Column chromatography (CC) purification was performed using silica gel (160–200 mesh), Sephadex LH-20 (GE, Boston, MA, USA) and C18 (50 μm, YMC, Kyoto, Japan). CC fractions were analyzed by thin-layer chromatography (TLC) (silica gel GF254).
4
Spectroscopic Analysis of Bioactive Compounds
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ORs were measured using a Rudolph Research Autopol III automatic polarimeter. UV, CD, and IR spectra were recorded using a Cary 300 spectrometer, a JASCO J-815 CD spectrometer, and a Nicolet 5700 FT-IR spectrometer (FT-IR microscope transmission), respectively. 1H and 13C NMR spectra were obtained at 600 and 150 MHz, respectively, using a Bruker-AVIIIHD-600 spectrometer with solvent peaks used as references. HR-ESIMS data were measured using a Micromass Autospec-Ultima ETOF spectrometer and Thermo LTQ Orbitrap XL mass spectrometer. Column chromatography was performed using silica gel (200–300 mesh, Qingdao Marine Chemical Inc., China) and MCI gel CHP20P (Mitsubishi Chemical Corporation, Tokyo, Japan). HPLC separation was performed using an Agilent 1200 series (quaternary pump, autosampler, diode array detector) with a Shiseido Capcell-Pak C18 MGII column (5 μm, 250 × 10 mm). TLC was performed using glass-precoated silica gel GF254 plates (Qingdao Marine Chemical Inc., Qingdao, China). Cervical cancer (Hela), hepatocellular carcinoma (HepG2), and human osteosarcoma (U2OS) cell lines were obtained from the National Infrastructure of Cell Line Resource (Beijing, China).
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