Szh10 stereo microscope

Callus tissue and the T1 seeds of pKL2155 were screened for tdTomato gene expression using fluorescent microscope (Olympus SZH10 stereo microscope with Texas red filter, EX 535–585nm, EM 605–690nm) or using a NIGHTSEA dual fluorescent protein flashlight and filter glasses (NIGHTSEA LLC, Lexington, MA, United States).
Putative transgenic T0, T1, and T2 plants generated from the bombardment of pAHC25 were phenotyped using GUS stain as described (Jefferson et al., 1987 (link)).
pAHC25 transgenic T2 progeny was tested for herbicide resistance using the protocol described previously (Frame et al., 2006 (link)). For screening of glufosinate tolerance, fully expanded leaves from 2-week-old teosinte seedlings were used. Glufosinate solution (150mg/L glufosinate plus 0.1% Tween 20; freshly prepared from the herbicide Liberty®, BASF, Ludwigshafer, Germany) was applied onto one-third of the leaf surface (from tip of the leaf). Q-tips soaked with the glufosinate solution were used to gently rub both sides of the leaves to ensure the herbicide contact and penetration. Plants were assessed for damage 2days after the leaf painting.

The proliferative capacity of lentivirus-infected LUAD cells transfected with or without SP3 expression plasmid or vector control for 48 h was analyzed according to the manufacturer’s instructions of the Cell-lightTM EdU DNA cell proliferation kit (Cat#C10310-3, Ribobio). Briefly, LUAD cells (1×10⁴) were seeded into 96-well plates. On the second day, the cells were incubated with 10 μM EdU for 2 h. After fixation with 4% paraformaldehyde (Cat#P1110, Solarbio), the cells were stained with Apollo Dye Solution. The nuclei of the cells were stained with Hoechst 33342. Image acquisition was performed using an Olympus SZH10 Stereo microscope (Olympus, Tokyo, Japan).

Under stereomicroscope viewing, hearts were placed ventral-side-up in a glass specimen chamber, which was then placed on a micromanipulator stage in the experimental setup as previously described [24 (link)]. The pipette and heart were aligned in view of an Olympus SZH10 stereomicroscope with the pipette perpendicular to the desired heart surface. As the pipette was lowered toward the heart surface, natural adhesion between the tissue and the glass formed an airtight seal. The test consisted of vacuum pressure applied to the pipette at a continual rate of 0.4 kPa/s, which aspirated tissue into the pipette (Fig 2). A digital camera (DCC1240M-GL, Thorlabs) mounted on the stereomicroscope captured images of the pipette tip and surrounding tissue at 140x magnification while vacuum pressure was applied. The data was recorded by a custom MATLAB program that simultaneously captured the voltage output from the pressure transducer and an image from the camera at a rate of 15 Hz. Three areas were sequentially tested on each heart: left ventricle (LV), right ventricle (RV), and outflow tract (OFT), at the locations shown (Fig 1).

Cells were seeded (1 × 10³ cells per well) in 96-well plates in growth media on day 0. At the time of harvest cells were fixed in 10% formalin, stained with 0.5% crystal violet (Sigma, Cat#V5265). crystal violet-stained cells were dissolved in 100% methanol as described previously⁴³ . For anchorage-independent cell growth assays, 0.5 ×10⁴ cells were plated in six-well 35 mm tissue culture dishes containing a bottom layer of 0.7% (w/v) agarose in complete medium and a top layer of 0.3% agarose solution mixed with cells and incubated at 37^oC for 3 weeks. Colonies were imaged using an Olympus SZH10 Stereo microscope (Olympus, Japan) and a Nikon D3000 camera (Nikon, Japan).

Cross-sections of eyes containing tumors were examined for the presence of the described markers with a BX51 Olympus upright fluorescence microscope (Olympus American Inc., Melville, NY). All fluorescent images were obtained at 200x magnification using controlled filters to visualize DAPI, (mamely. Alexa Fluor 488, and 568 signals). Light micrographs of tumor burden were imaged at 40X with the Olympus SZH10 stereo microscope.